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Phase 2a - Microbiology > Bacteriology > Flashcards

Bacteriology Flashcards

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Biology 101 flashcards
1
Q

What colour do gram positive bacteria stain?

A

Purple

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2
Q

What colour do gram negative bacteria stain?

A

Pink

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3
Q

Name the two clinically important aerobic cocci geni

A

1) Staphylococcus

2) Streptococcus

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4
Q

Name the two clinically important Staphlococci

A

1) S. aureus

2) S. epidermidis

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5
Q

What are streptococci sub-divided into?

A

1) Beta-haemolytic
2) Alpha-haemolytic
3) Non-haemolytic

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6
Q

What medium does haemolysis occur on?

A

Blood agar

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7
Q

What is the appearance of beta-haemolysis?

A

Transparent when held up to the light

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8
Q

What is the appearance of alpha-haemolysis?

A

Translucent and green

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9
Q

What further test would you do to identify alpha-haemolytic Streptococcus pneumoniae?

A

Optochin test

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10
Q

Is S. pneumoniae sensitive or resistant to the optochin test?

A

Sensitive

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11
Q

What test would you do to distinguish between Staphlococci?

A

Coagulase test (or DNAse)

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12
Q

Which Staphlococcus is coagulase positive?

A

S. aureus

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13
Q

Which Staphlococci are coagulase negative?

A

Multiple including S. epidermidis

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14
Q

What happens if you carry out a coagulase test with S. aureus?

A

The solution solidifies (positive)

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15
Q

What happens if you carry out a coagulase test with S. epidermidis?

A

It remains liquid (negative)

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16
Q

How can you distinguish between gram negative bacilli?

A

Lactose fermentation test

17
Q

Is Shigella lactose fermenting or non-lactose fermenting?

A

Non-lactose fermenting

18
Q

Is E.coli lactose fermenting or non-lactose fermenting?

A

Lactose fermenting

19
Q

Is salmonella lactose fermenting or non-lactose fermenting?

A

Non lactose fermenting

20
Q

How do you distinguish between non-lactose fermenting gram negative bacilli?

A

Oxidase test

21
Q

Which gram negative bacilli have a positive oxidase test?

A

Pseudomonas

22
Q

What bacteria are resistant to the optochin test?

A

Viridans Strep

23
Q

What is the appearance of S. aureus on agar?

A

Golden colonies

24
Q

What is the appearance of S. epidermidis on agar?

A

White colonies

25
Q

What is the purpose of the Lancefield test?

A

To differentiate between beta-haemolytic Streptococci

26
Q

Name the three main plates?

A

1) Blood agar
2) McConkey
3) XLD

27
Q

Which is the least selective plate?

A

Blood agar

28
Q

What bacteria show in pink colonies on XLD?

A

Shigella and Salmonella

29
Q

What two colours do bacteria grow on XLD?

A

Yellow and Pink

Phase 2a - Microbiology (2 decks)

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