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Micro 1 > Bacteriology > Flashcards

Bacteriology Flashcards

1
Q

Gram positive cocci except

A

Nisseria
Moraxella
Vionella

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2
Q

Alpha, beta and gamma hemolysis—
Type of hemolysis and halo color formed

A

Alpha - partial and green halo
Beta- complete and colorless halo
Gamma- no hemolysis and no color

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3
Q

Catalase positive and negative bacteria

A

Positive- staphylococcus
Neg- strept

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4
Q

Coagulase test positive and negative bacteria?

A

Positive- S. Aureus
Neg- CONS

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5
Q

Novobiocin sensitive and resistant bacteria?

A

Resistant - staph saprophyticus
Sensitive- staph epidermidis

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6
Q

S aureus and s epidermidis differentiating screening procedure

A

Phosphatase rxn
S aureus - pink in this

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7
Q

M/c cause of pus Formn in human body

A

Staphylococcus

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8
Q

Staph discovered by

A

Alexander

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9
Q

Wet prepn examples-

A

Hanging drop technique
Dark field microscopy

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10
Q

Which bacteria is seen in dark field microscopy

A

Spirochetes

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11
Q

Wet prepn adv?

A

Motility of bacteria is seen

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12
Q

Contrast study or stain prepn
Examples and adv

A

4 stain tech
Adv - 1 morphology of bacteria seen
2 colony formn

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13
Q

4 types of staining tech and examples

A

Simple stain - methylene blue and crystal violet
Impregnating stain- silver staining
Neg stain- Indiana ink and negrosin
Differential stain- gram staining

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14
Q

Gram staining was discovered by

A

Christian gram

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15
Q

Colors of gram staining and its diagnosis

A

Purple - gram positive
Red - gram negative

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16
Q

Cause of positive gram staining in bacteria

A

Peptidoglycan

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17
Q

ZN staining colors and diagnosis

A

Red - acid fast bacteria
Blue/ green- non acid fast bacteria

18
Q

Cause for color in zn staining

A

Mycolic acid

19
Q

Steps in gram staining and zn staining

A

Gram staining- Zn staining
1st - crystal violet. Carbol fuschin
Fixer- iodine. Heat
Decolorizer- acetone/alcohol. H2so4

2nd stain- carbol fuschin or saffranin Malachite green/ methylene blue- zn stain

20
Q

Strong acid fast bacteria example
Weak acid fast

A

Strong - m. Tb
Weak- nocardia and muco leprae

21
Q

Parts of bacterial growth curve

A

Lag
Log
Stationary
Death

22
Q

Increase in enzymes is seen in which part of curve

A

Lag phase

23
Q

Bacterial cell adaptation is seen in which phase

A

Lag phase

24
Q

Cell division of bacteria is seen in which phase

A

Log

25
Q

Maximum no of bacteria is seen in which phase

A

End of lag phase

26
Q

Which phase shows more susceptibility to antibiotics

A

Log phase

27
Q

AMA’s should be used in which phase

A

Log phase

28
Q

Size of bacteria increases or decreases in log phase

A

Decreases

29
Q

Why is there a stationary phase

A

No of dead bacteria =no of viable bacteria

30
Q

Reason for stagnation growth

A

Due to low availability of nutrients

31
Q

Phenomenon which occur in stationary phase

A

Sporulation exotoxin production

32
Q

Which bacteria’s have capsules

A

Ye yersinia
P pseudomonas
A anthrax
K klebsiella
I h influenza
M meningococci
C cryptococcus
B bordetella
C clostridium
Va vibrio parahemolyticus

33
Q

Main function of capsule

A

Antiphagocytic
Virulence
Biofilm formation

34
Q

All bacteria capsule are made of what

A

Polysaccharide

35
Q

B anthrax capsule is made of

A

Polypeptide

36
Q

Capsular demonstration

A

Quellung rxn- cap qb protein
Macfyden rxns- seen in b anthrax

37
Q

Examples of bacteria w spore

A

B - b anthrax
S - sporosarcina
C - clostridium
Chemistry - coxiella burnetti

38
Q

Types of flagella in bacteria

A

Polar and peritrichous

39
Q

Polar flagella examples

A

V vibrio
L legionella
C campylobacter
H h pylori
P pseudomonas
S spirochetes

40
Q

Peritrichous flagella examples

A

C clostridium
B bacillus except anthrax
S salmonella
E e coli
Padh proteus
Le leisteria

41
Q

All bacilli are gram neg except

A

A actinomycetes
B bacillus anthrax
C clostridium
D diphtheria
L listeria
M mycobacterium
N nocardia

Micro 1 (2 decks)

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