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MTAP1.3 - MIDTERMS > Bacteriology > Flashcards

Bacteriology Flashcards

1
Q

Antibiotic agents classified as cell wall inhibitors
1. clarithromycin
2. Vancomycin
3. Tetracycline
4. Imipenem

A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these

A

E. None of these

vancomycin only

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2
Q

Non-encapsulated N. meningitis
A. Throat swab
B. Nasopharyngeal swab
C. CSF
D. A and B

A

D. A and B

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3
Q

Biosafety Lab mode of transmission gram + afb
A. BSL 1 B. BSL 2 C. BSL 3 D. BSL 4

A

C. BSL 3

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4
Q

Choose the correct order of specimen - understaffed

A

Pericardial fluid
unpreserved stool
colony counting

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5
Q

Delay CSF temp storage

A

37C

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6
Q

Kirby Bauer
Intermediate- 12-14 mm
_________- 16 mm zone of inhibition result

A

susceptible

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7
Q

Blood submitted for culture (+) S. aureus
A. Repeated since S. aureus is not significant
B. serial specimen must be collected
C. Communicated immediate
D. all of these

A

C. Communicated immediate

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8
Q

Resazurin inside that contains inoculated plates must appear:

A

pink

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9
Q

Repeated test for UTI that anaerobes agent
A. First morning
B. random urine
C. Catheterized
D. none of these

A

C. Catheterized

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10
Q

Days of culture bottle for growth

A

7 days

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11
Q

Sputum for mycobacteria detection must be treated with which of
the following prior to doing centrifugation?
A. NaOH
B. NACL
C. Z-TSP
D. Any of these

A

D. Any of these

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12
Q

Which of the following cells that when noted microscopically would
confirm that specimen is a true respiratory sample
A. Columnar cells
B. Mast cells
C. Neutrophils
D. None of these

A

C. Neutrophils

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13
Q

Which of the following that when noted in the laboratory will
warrant immediate communication of result to the clinician?
1. Surgical wound + streptococcus pyogenes
2. Positive acid fast stain
3. Gram stain suggestive of gas gangrene
4. Positive blood culture

A. 1 and 3
B. 2 and 4
C. 1, 2, 3
D. 1,2,3,4
E. None of these

A

B. 2 and 4

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14
Q

When doing AST Kirby Bauer Disk method what is the maximum
number of antibiotic disks which may be applied to the surface of a single 150 mm Mueller Hinton Agar plate?

A

12 disks

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15
Q

Which of the following antimicrobials are classified as Macrolides?
1. Clarithromycin
2. Gentamycin
3. Erythromycin
4. Imipenem

A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these

A

A. 1 and 3

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16
Q

Kirby bauer Technique, too light inoculum and very dry agar can lead
to?
A. Smaller zone - false resistance
B. Smaller zone - false susceptible
C. Larger zone - false susceptible
D. Larger zone - false resistance

A

C. Larger zone - false susceptible

17
Q

are true of the D test for staphylococcus macrolide resistance except:
A. – and 2ug CC disk dispersed 15 mm apart
B. Detects inducible clindamycin resistance
C. — e zone constitutes to a positive result
D. Performed when macroslide results are discrepant

A

D. Performed when macroslide results are discrepant

18
Q

–to cause a false resistant result in disk diffusion method susceptible
testing except:
A. -
B. Too heavy inoculum
C. E-test
D. None of these

A

C. E-test

19
Q

Candle jar contain

A

5-10% CO2, 5% O2

20
Q

Inhibition of MIC and MBC

A

E-TEST

21
Q

Failure in gaspak jar:
A. presence air in colorless
B. Inactivation of catalyst due to repeated use
C. Both

A

B. Inactivation of catalyst due to repeated use

22
Q

– of the following materials may be used in specimen collection for
N. meningitidis except:
A. Calcium alginate
B. Swab made of Dacron
C. Swab made of rayon fibers
D. All of these

A

C. Swab made of rayon fibers

23
Q

The gene that activates resistance to clindamycin is
A. Emm gene
B. Erm gene
C. mecA gene

A

B. Erm gene

24
Q

Blood culture contaminants
A. S aureus
B. P acnes
C. C viridans
D. All of these

A

D. All of these

25
Q

Blood anticoagulant not suited for bacterial culture
A. Citrate
B. Heparin
C. EDTA
D. A and C

A

D. A and C

26
Q

105 colonies were counted on a plate media with a request of urine
C/S, if a 1 uL loop was used during inoculation on media, what is the
colony count/ml of urine and correct interpretation?

A. 105,000 - may be considered UTI
B. 10,500 - not UTI
C. 115,000 - normal finding
D. None of these

A

A. 105,000 - may be considered UTI

27
Q

All of the following are UTI agents except:
A. S. pneumoniae
B. K. pnuemoniae
C. E. coli
D. S. saprophyticus
E. A and B

A

E. A and B

28
Q

Possible isolates in CSF culture
1. S. pneumoniae
2. S. agalactiae
3. N. meningitidis
4. H. influenza

A

ALL

29
Q

Semiquantitation technique involves streaking on

A

4 QUADRANTS

30
Q

Clindamycin result after a (+) D test
A. Susceptible
B. Resistant
C. Intermediate
D. Repeat D-test

A

B. Resistant

31
Q

Swarming on a Kirby Bauer plate must be

A

Ignore

32
Q

Which of the following agents must be added to a fungal media?
1. Cycloserine
2. Chloramphenicol
3. Cefoxitin
4. Cycloheximide

A

2 and 4

33
Q

What is gram staining reaction of fungi

A

Gram positive

34
Q

Which of the following can be used for aspergillus?

A

Czapeck Agar

35
Q

which can be used to stimulate chlamydospore formation of C. albcans?

A

Cornmeal Agar

36
Q

Which of the following permanent mounts can be used to
demonstrate fungi in tissue samples?
1. H&E
2. Periodic Acid Schiff
3. Gomori’s methenamine silver
4. Fontana Masson

A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these

A

E. None of these

37
Q

Demonstration of C. neoformans capsule may be accomplished thru
the use of
A. Calcofluor white
B. Lactophenol cotton blue
C. Acridine orange
D. None of these

A

D. None of these

MTAP1.3 - MIDTERMS (5 decks)

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