Bacteriology Flashcards
Antibiotic agents classified as cell wall inhibitors
1. clarithromycin
2. Vancomycin
3. Tetracycline
4. Imipenem
A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these
E. None of these
vancomycin only
Non-encapsulated N. meningitis
A. Throat swab
B. Nasopharyngeal swab
C. CSF
D. A and B
D. A and B
Biosafety Lab mode of transmission gram + afb
A. BSL 1 B. BSL 2 C. BSL 3 D. BSL 4
C. BSL 3
Choose the correct order of specimen - understaffed
Pericardial fluid
unpreserved stool
colony counting
Delay CSF temp storage
37C
Kirby Bauer
Intermediate- 12-14 mm
_________- 16 mm zone of inhibition result
susceptible
Blood submitted for culture (+) S. aureus
A. Repeated since S. aureus is not significant
B. serial specimen must be collected
C. Communicated immediate
D. all of these
C. Communicated immediate
Resazurin inside that contains inoculated plates must appear:
pink
Repeated test for UTI that anaerobes agent
A. First morning
B. random urine
C. Catheterized
D. none of these
C. Catheterized
Days of culture bottle for growth
7 days
Sputum for mycobacteria detection must be treated with which of
the following prior to doing centrifugation?
A. NaOH
B. NACL
C. Z-TSP
D. Any of these
D. Any of these
Which of the following cells that when noted microscopically would
confirm that specimen is a true respiratory sample
A. Columnar cells
B. Mast cells
C. Neutrophils
D. None of these
C. Neutrophils
Which of the following that when noted in the laboratory will
warrant immediate communication of result to the clinician?
1. Surgical wound + streptococcus pyogenes
2. Positive acid fast stain
3. Gram stain suggestive of gas gangrene
4. Positive blood culture
A. 1 and 3
B. 2 and 4
C. 1, 2, 3
D. 1,2,3,4
E. None of these
B. 2 and 4
When doing AST Kirby Bauer Disk method what is the maximum
number of antibiotic disks which may be applied to the surface of a single 150 mm Mueller Hinton Agar plate?
12 disks
Which of the following antimicrobials are classified as Macrolides?
1. Clarithromycin
2. Gentamycin
3. Erythromycin
4. Imipenem
A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these
A. 1 and 3
Kirby bauer Technique, too light inoculum and very dry agar can lead
to?
A. Smaller zone - false resistance
B. Smaller zone - false susceptible
C. Larger zone - false susceptible
D. Larger zone - false resistance
C. Larger zone - false susceptible
are true of the D test for staphylococcus macrolide resistance except:
A. – and 2ug CC disk dispersed 15 mm apart
B. Detects inducible clindamycin resistance
C. — e zone constitutes to a positive result
D. Performed when macroslide results are discrepant
D. Performed when macroslide results are discrepant
–to cause a false resistant result in disk diffusion method susceptible
testing except:
A. -
B. Too heavy inoculum
C. E-test
D. None of these
C. E-test
Candle jar contain
5-10% CO2, 5% O2
Inhibition of MIC and MBC
E-TEST
Failure in gaspak jar:
A. presence air in colorless
B. Inactivation of catalyst due to repeated use
C. Both
B. Inactivation of catalyst due to repeated use
– of the following materials may be used in specimen collection for
N. meningitidis except:
A. Calcium alginate
B. Swab made of Dacron
C. Swab made of rayon fibers
D. All of these
C. Swab made of rayon fibers
The gene that activates resistance to clindamycin is
A. Emm gene
B. Erm gene
C. mecA gene
B. Erm gene
Blood culture contaminants
A. S aureus
B. P acnes
C. C viridans
D. All of these
D. All of these
Blood anticoagulant not suited for bacterial culture
A. Citrate
B. Heparin
C. EDTA
D. A and C
D. A and C
105 colonies were counted on a plate media with a request of urine
C/S, if a 1 uL loop was used during inoculation on media, what is the
colony count/ml of urine and correct interpretation?
A. 105,000 - may be considered UTI
B. 10,500 - not UTI
C. 115,000 - normal finding
D. None of these
A. 105,000 - may be considered UTI
All of the following are UTI agents except:
A. S. pneumoniae
B. K. pnuemoniae
C. E. coli
D. S. saprophyticus
E. A and B
E. A and B
Possible isolates in CSF culture
1. S. pneumoniae
2. S. agalactiae
3. N. meningitidis
4. H. influenza
ALL
Semiquantitation technique involves streaking on
4 QUADRANTS
Clindamycin result after a (+) D test
A. Susceptible
B. Resistant
C. Intermediate
D. Repeat D-test
B. Resistant
Swarming on a Kirby Bauer plate must be
Ignore
Which of the following agents must be added to a fungal media?
1. Cycloserine
2. Chloramphenicol
3. Cefoxitin
4. Cycloheximide
2 and 4
What is gram staining reaction of fungi
Gram positive
Which of the following can be used for aspergillus?
Czapeck Agar
which can be used to stimulate chlamydospore formation of C. albcans?
Cornmeal Agar
Which of the following permanent mounts can be used to
demonstrate fungi in tissue samples?
1. H&E
2. Periodic Acid Schiff
3. Gomori’s methenamine silver
4. Fontana Masson
A. 1 and 3
B. 2 and 4
C. 1,2,3
D. 1,2,3,4
E. None of these
E. None of these
Demonstration of C. neoformans capsule may be accomplished thru
the use of
A. Calcofluor white
B. Lactophenol cotton blue
C. Acridine orange
D. None of these
D. None of these
