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Bacterias Flashcards

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1
Q

Enterobacteriaceae

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram-
  2. Facultative anaerobic
  3. Oxidase-, Catalase vary
  4. Glucose fermentation
  5. VRGB agar, EE Broth
  6. Glucose Fermentation test, oxidase test (-)
  7. Very
  8. Reduce nitrate to nitrite
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2
Q

E. coli

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram-
  2. Facultative anarobic
  3. Catalase+, Oxidase-
  4. Fermentation?
  5. TBX agar, MMG broth, Brilliant green-bile-lactose broth
  6. Tests: Citrate utilization test -, Voges-Proskauer test -, Methyl red test +, Indole test +, IMVIC tests
  7. Mesophilic
  8. Other important
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3
Q

Enterococcus faecialis

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram+
  2. Facultative, Aerotolerant anaerobic
  3. Catalase- (+ in some conditions), Oxidase-
  4. Ferments glucose
  5. Slanets-Bartley agar
  6. Tests: Heat resistance examination
  8. Catalase-
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4
Q

Salmonella

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram-
  2. Facultative anarobic
  3. Catalase+, Oxidase-
  4. H2S prod, decompose glucose
  5. RVS Broth, MKTTn, XLD, BPLactose, BPLSucrose, MSRV
  6. Tests: Lysine decarboxylation +, Methyl red test+, TSI+, Urea test-, Indol-, Voges Proskauer Test-, ONPG Test-
  7. Mesophilic
  8. Other important
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5
Q

Listeria Monocytogenes

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram+
  2. Aerobic or facultative anaerobic bacteria
  3. Catalase+, Oxidase-
  4. Ferments glucose+maltose
  5. Aloa agar, Oxford agar, Palcalm agar, Fraser broths,
  6. CAMP test: S.aureus (+), Rh.equi (-), Catalase Test+, Motility test+, Haemolysis test+, Acid Prod. from CHO: rhamose (+) xylose (-),
  7. Psychrophilic
  8. B-Haemolysis+, Rhamnose+, Somersaulting motility 20-25C,
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6
Q

Staphylococcus aureus

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram+
  2. Aerobic or facultative anaerobic bacteria
  3. Catalase+, Oxidase-
  4. Ferments lactose, sucrose, maltose
  5. Giolitti- Cantoni broth, Baird-Parker agar,
  6. Coagulase+, Thermostable deoxyribonuclease test
  7. Mesophilic
  8. Reduces nitrate to nitrite, B-Haemolysis+,
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7
Q

Cl. perfringens

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram+
  2. Anaerobic
  3. Oxidase-, Catalase-
  4. Fermentation?
  5. Sulphite cycloserine agar, Lactose-sulphite broth
  6. Fluid thioglycolate medium,
  7. Temp.?
  8. Spore forming, haemolysis+
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8
Q

Cl botulinum

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram+
  2. O2 requirement?
  3. Oxidase-, Catalase-
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Group 1 mesophilic, Group 2 psychrophilic
  8. Spore forming
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9
Q

Bacillus cereus

  1. Gram?
  2. O2 requirement?
  3. Oxidase/Catalase?
  4. Fermentation?
  5. Agars? Broths?
  6. Tests?
  7. Temp.?
  8. Other important
A
  1. Gram+
  2. Facultative anaerobic
  3. Catalase+, oxidase-
  4. Decompose mannitol
  5. MYP agar
  6. Tests?
  7. Temp.?
  8. Lecithinase+, Phosphorylase+, Reduce nitrate to nitrite, Hemolysis+, Emetic toxin
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10
Q

Which bacterias are Fresh Faecal Contamination Indicators?

A

E. coli, Enterobacteriaceae, Enterococcus Faecalis (long term)

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11
Q

Which bacterias are psycrophilic?

A

Listeria monocytogenes
Yersinia enterocolitica
Clostridium botulinum (group 2)
Some strains of: Bacillus cereus / E.coli

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12
Q

Which bacterias are mesophilic?

A

Salmonella
E. coli
S. aureus
Clostridium botulinum (group 1)

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13
Q

Which bacterias are thermophilic?

A

Campylobacter

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14
Q

Optimal for multipilcation of bacterias

A

neutral pH (6.5-7.5),

temp. : psychrofilic - thermophilic
nutrients: rich in protein

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15
Q

Optimal for multipilcation of yeasts

A

low pH (4.5-6.5),

temp. : psychrophilic, mezophilic,
nutrients: rich in carbohydrate

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Food Hygiene (3 decks)

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