Bacterial Taxonomy Flashcards
An area of biological science comprising of CLASSIFICATION, NOMENCLATURE and IDENTIFICATION of organisms
Taxonomy
Provides a consistent means to organize, name
, and identify organisms
Taxonomy
Provides a common label for every organism
Taxonomy
Organization of
microorganisms that
share similar
morphologic,
physiologic, and
genetic traits into
specific groups or
taxa
Classification
Enumerate the 7 taxa designations in classifying organisms:
Kingdom
Division
Class
Order
Family
Genus
Species
It is the most basic taxonomic group
Species
Collection of bacterial strain w/ common physiologic and genetic features
Species
taxonomic
subgroup w/in a species
Subspecies
groups below
the subspecies level that
share minor but specific characteristics
Biotype
Serotype
Genotype
They established rules and guidelines for naming of microorganisms
International Code of Nomenclature of Bacteria (ICNB) or Bacteriological Code (BC)
He established the nomenclature system in 1735
Carolus Linnaeus
System used for naming microorganisms
Binomial system
Process by w/c a microorganism’s key features are delineated
Identification
Enumerate the 2 identification methods
Genotypic characteristics
Phenotypic characteristics
genetic makeup
- DNA base composition ratio
- Nucleic acid base sequence analysis
Genotypic characteristics
genetic makeup
- DNA base composition ratio
- Nucleic acid base sequence analysis
Genotypic characteristics
Observable characteristics
- Macroscopic morphology
- Microscopic morphology
- Staining characteristics
- Environmental requirements
- Nutritional requirements
- Resistance profiles
- Antigenic properties
- Subcellular properties
Phenotypic characteristics
Principle: Characteristics of microbial growth pattern on culture media
Macroscopic morphology
Examples of macroscopic morphology
Size, shape, texture, pigmentation of colonies
Size, shape, intracellular inclusions, cellular appendages, and
arrangement of cells under the microscope
Microscopic morphology
Staining reaction/affinity to dyes as an aid to identification
Staining characteristics
Examples of tests for staining characteristics
Gram stain
Acid fast stain
Ability of organism to grow at various temperatures, in the presence of oxygen and other gases, at various pH levels etc
Environmental requirements
Exhibition of a characteristic inherent resistance to specific
antibiotics, heavy metals, or toxins by certain microorganisms
Resistance profiles
Profiling microorganisms by various serologic and immunologic
methods
Antigenic properties
Establishment of molecular constituents like components of the:
cell wall, cell membrane and enzymatic contents
Subcellular properties
What are the 3 areas of microbial taxonomy?
Classification
Nomenclature
Identification
The term “epithet” refers to which taxonomic group?
Species
Subgrouping of bacteria that share similar
serologic characteristics is called
__________________
Serotype
Subgrouping of bacteria that share similar
biological characteristics is called __________________
Biotype
Subgrouping of bacteria that share some
similar genetic sequences is called _________________.
Genotype
Which of the following is the correct nomenclature?
A. Pseudomonas Aeruginosa
B. pseudomonas aeruginosa
C. PSEUDOMONAS AERUGINOSA
D. Pseudomonas aeruginosa
D. Pseudomonas aeruginosa
what phenotypic criteria is applied to gram staining
Staining characteristics
what phenotypic criteria is applied to Ziehl-Neelsen Technique
Staining characteristics
what phenotypic criteria is applied to catalase test
Subcellular properties
what phenotypic criteria is applied to Kirby - Bauer diffusion test
Resistance profiles
what phenotypic criteria is applied to incubation using candle jar
Environmental requirements
what phenotypic criteria is applied to the detection of pink, mucoid colonies on MAC
Macroscopic morphology
what phenotypic criteria is applied to the determination of hemolytic patterns on BAP
Macroscopic morphology
what phenotypic criteria is applied to Widal test
Antigenic properties
what phenotypic criteria is applied to rapid plasma reagin test
Antigenic properties
what phenotypic criteria is applied to elek test
Resistance profiles