BACTERIAL ISOLATION

Question 1

Bacterial isolation involves 5 aspects. What are those?

Answer 1

1. Specimen
2. Collection
3. Preservation
4. Culturing
5. Microscopic examination

Question 2

This isolation method is a popular method for pure culture; to perform, fresh nutrient agar is poured onto a sterile petri dish until it solidifies

Bacterial samples are streaked across the surface of the petri plate using an inoculating loop/

Answer 2

Streaking metod

Question 3

Primary method to separate different groups of microorganism; method that allows us to determine different groups of bacteria based on growth patterns

Answer 3

Bacterial isolation

Question 4

This isolation method is processed as bacterial suspension diluted serially in successive test tubes.

Answer 4

Serial dilution method

Question 5

This isolation method is done when a nutrient medium is added to the petri dish before adding to the bacterial sample

After solidifying, the bacterial suspension is added and spread throughout the medium’s surface.

Answer 5

Spreading method

*** For uniform distribution, use T or L shape spreader

Question 6

This isolation method is the simplest method for bacterial isolation; bacterial suspension laden with a huge bacterial population is generally taken

Answer 6

Pouring method

PROCEDURE:
- Using a pipette, take 1 mL of bacteria sample into the sterile petri plate. (there should be nutrient source like carbon and nitrogen)
- For uniform distribution, rotate the plates in clockwise and counter clockwise direction
- Allow the culture plates to solidify before incubating at 35-37 degrees Celsius for 48 hrs.