Bacterial foodborne pathogens Flashcards

1
Q

Which bacterial foodborne pathogens are gram negative?

A
Salmonella
Campylobacter
E.coli 
Shigella
Yersinia
Vibrio
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2
Q

Which bacterial foodborne pathogens are gram positive?

A

Listeria
Staphylococcus
Clostridium
Bacillus

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3
Q

What is foodborne intoxication?

A

Organism produces toxins that are ingested

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4
Q

Does foodborne intoxication have a slower or faster onset?

A

Rapid onset

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5
Q

What are the two types of food borne infection? What do these mean?

A

Invasive infection - bacteria ingested in food and penetrate intestinal mucosa (can cause local or systemic disease)
Toxicoinfection - bacteria ingested in food and produce toxins in the GI tract

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6
Q

What bacteria causes Yersioniosis? Cool fact about Yersinia?

A

Yersinia enterocolitica

Another species caused the black death

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7
Q

Yersinia is the 3d most common food disease. Is it’s bacteria gram positive or negative? What shape is it? Is it motile?

A

Gram negative
Rod shaped - NON spore forming
Motile at room temperature only - not body temp

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8
Q

Where is Yersinia enterocolitica found?

A

Throat, tonsils and faeces of PIGS

avoid during evisceration

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9
Q

How is yersioniosis transmitted?

A

Faecal oral route

Asymptomatic carriers

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10
Q

What symptoms does Yersioniosis cause?

A

Fever
Gastroenteritis with haemorrhage diarrhoea
Pseudo-appendicitis

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11
Q

Vibrio species are what type of bacteria? (O2 requirement, shape, salt requirement)

A

Facultative anaerobes
Straight or curved rods
Halophilic - need NaCl

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12
Q

What is the primary source of Vibrio infection?

A

Contaminated water or shellfish

Poor sanitation

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13
Q

What bacteria causes cholera? What is the incubation period and symptoms?

A

Vibrio cholerae
6 hrs
Severe pain, profuse watery diarrhoea, vomiting, dehydration, acidosis shock and death if not rehydrated

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14
Q

How can Vibrio bacteria be controlled?

A

Consume clean water
Dispose of sewage correctly
Don’t eat seafood from waters containing cholera

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15
Q

What are the two other species of Vibrio (except cholerae). Which is mild and which is severe?

A

Vibrio parahaemolyticus - mild

Vibrio vulnificus - severe

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16
Q

What bacteria causes invasive listeriosis infection? What is the main source of this bacteria (organisms)?

A
Listeria monocytogenes
Animal intestines (asymptomatic carries) and humans
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17
Q

Is listeria motile at room or body temperature? What is it very resistant to?

A

Room temperature = motile

Desiccation

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18
Q

What foods can listeria monocytogenes be found in?

A

Raw milk
Soft cheese
Vegetables
Unpasteurised juice

19
Q

What is the incubation period of invasive listeriosis? How does it enter the CNS?

A

Up to 21 days

Invades GI mucosa, enters macrophages which go through the bloodstream to the CNS

20
Q

What are the symptoms of invasive listeriosis?

A

Septicaemia
Meningitis
Encephalitis
Abortion

21
Q

What are the symptoms of non-invasive listeriosis?

A

Diarrhoea
Mild fever
Headache
Myalgia (muscle pain)

22
Q

How long is the incubation period of non-invasive listeriosis?

A

Much shorter = 1-3 days

23
Q

Where is staph aureus found and what is the name of the disease it causes?

A

Skin, hands, nares, milk, environment

Staphyloenterotoxicosis

24
Q

Is staph aureus motile or non-motile? What air conditions does it prefer? What is it very resistant to?

A

Non-motile
Facultative anaerobe
Desiccation (enterotoxin also resistant to heat)

25
Q

Which bacteria are resistant to desiccation?

A

Listeriosis

Staph aureus

26
Q

How many toxins does staph aureus produce? Which are the most common?

A

8

A and B

27
Q

What is the incubation period of staphyloenterotoxicosis? What are the symptoms

A

1-6 hrs
NO FEVER
D+, V+, abdo pain

28
Q

What bacteria causes botulism and infant botulism? What foods is it associated with?

A

Clostridium botulinum

Honey, poorly canned foods

29
Q

What environment does clostridium botulinum need to form spores? How does it cause cell death?

A

Anaerobic

Produces toxins which block nerves - paralysis and death

30
Q

What is the difference between botulism and infant botulism?

A

Botulism - intoxication, affects adults

Infant botulism - toxico-infection, children <1

31
Q

Why are children more susceptible to infant botulism? What ar eat symptoms?

A

Don’t have established gut flora

Constipation, respiratory distress

32
Q

How long is the incubation period of Botulism?

A

1-3 days

33
Q

Are botulism spores heat resistant? How can botulism be prevented?

A

No

Toxins sensitive to heat - heat at 80 degrees for 30 mins

34
Q

What two syndromes does bacillus cereus form?

A

Emetic syndrome

Diarrhoeal syndrome

35
Q

What type of gram is bacillus cereus? What shape is it? Is it motile? Does it have spores/toxins?

A

Gram positive
Rod
Motile
Spores and toxins - v resistant to heat

36
Q

What foods are associated with bacillus cereus?

A

Rice

Pulses

37
Q

What type of infection is Bacillus cereus emetic syndrome? Can the cells be killed by cooking? What are the symptoms?

A

Intoxication
No - vegetative cells killed but spores and toxins are not
Rapid vomiting (within minutes)

38
Q

What type of infection is Diarrhoeal syndrome by bacillus cereus? How is it caused? What are the symptoms?

A

Toxico-infection
Spores/vegetative cells ingested and toxins produced in the GI
Short, profuse painful D+ within hrs

39
Q

Clostridium perfringens produce what type of toxin? What is interesting about this?

A

Type A toxin

Survives in GI tract = asymptomatic carriers

40
Q

What affect does cooking have on spores? Why?

A

Activates them

Germinate in anaerobic conditions

41
Q

What is the most common source of Clostridium perfringens?

A

Cooked meat

TURKEY

42
Q

What are the symptoms of Clostridium perfringens infection? How long does it last?

A

Severe abdo pain
D+++
2 days - fatal if immunocompromised

43
Q

How can foodborne bacterias be prevented?

A

Cook thoroughly
Rapid chill
Reheat to 75/80 degrees for over 30 mins