Bacterial Differentials (General) Flashcards
Gram Neg Diplococci
Neisseria
Gram Neg Cocobacilli
Acinetobacter
Morganella
Haemophilus (CHOC only, wet mouse)
Gram Neg Rods - lactose fermenters
E. coli Klebsiella Enterobacter Serratia Citrobacter
Gram Neg Rods - non-lactose fermenters
Pseudomonas (grape) Burkholderia Stenotrophomonas Salmonella Shigella
Curved Gram Neg Rod
Campylobacter
Helicobacter
Vibrio
Flexispira
Gram Neg Rods - special atmosphere
HACEK
Eikenella (bleach)
Gram Neg Rods - special media
Yersinia
Brucella
Fransicella
Gram Neg Rods - strict anerobe
Bacteroides
Fusobacterium
Gram Pos Cocci - clusters
Staph (catalase pos) Micrococcus Finegoldia Rothia Aerococcus Pediococcus
Gram Pos Cocci - pairs and chains
Streptococci (catalase neg) Enterococci (catalase neg) Leuconstoc Lactococcus Gemella Globictella Vagococcus
Gram Pos Rods - anaerobic
Clostridium
Proprionibacterium
Actinomyces
Gram Pos Rods - aerobic
Bacillus Corynebacterium Listeria Erysipelothrix Lactobacillus Arcanobacterium Mycobacteria (beaded) Nocardia (beaded)
Gram Pos Rods - beaded
Nocardia
Actinomyces (anaerobic)
Mycobacteria
Gram Pos Rods - pallisading
Corynebacterium
Arcanobacterium
Gram Pos Rods - box car shaped
Clostridium
Gram Pos Rods - Nest like
Proprionibacterium acnes
Which bug produces double-zone hemolysis?
Clostridium perfringens
Smells like Indole?
E. coli
Smells like grapes?
Pseudomonas
Smells like Butterscotch?
Strep. anginosis
Smells like wet mouse?
Haemophilus
Smells like bleach?
Eikenella
Which biochemical differentiates between Staph and Strep?
Catalase
Catalase positive organisms
Staphylococcus Micrococcus Listeria Bacillus Corynebacterium Most Gram Negs
Catalase positive GPCs
Micrococcus
Aliococcus
Staphylococcus
Rothia mucilaginosa
The CAMP test is for presumptive identification of what?
Group B Streptococcus (S. agalactiae)
CAMP positive organisms
GBS
Listeria
P. acnes
Turicella otitidis
Reverse CAMP test is specific for which orgs?
C. perfringens
Archanobacterium haemolyticum
What does hippurate hydrolysis test for?
The presence of hippurate hydrolase, which hydrolyses hippuric acid to benzoic acid and glycine
Hippurate hydrolysis positive orgs
GBS
Campylobacter jejuni
Lengionella pneumophila Gardnerella vaginalis Listeria monocytogenes Bacillus Aerococcus
Group A Strep (Morphology and defining biochemicals)
GPC, chains, “string of pearls” very round cells
Beta-hemolytic on SBA
Catalase neg, PYR pos, Bacitracin sensitive
Strep pneumoniae (Morphology and defining biochemicals)
Lancet shaped GPC pairs, with capsule Alpha-hemolytic on SBA Donut shaped (dent in middle) colonies Optichin sensitive (presumptive) Bile soluble (confirmatory)
Viridans Streptococci groups
Mutans Anginosis (milleri) Mitis Salivarius Bovis
Strep mutans group (characteristics)
Rod morphology off agar, cocci morphology off broth
Strep mutans group (species)
S. mutans
S. sobrinus
Strep anginosis group (characteristics)
Butterscotch odor
Strep anginosis group (species)
S. anginosis
S. constellatus
S. intermedius
Strep mitis group (characteristics)
Optichin sensitive, bile soluble
Strep mitis group (species)
S. mitis S. pneumoniae S. sanguinis S. gordonii S. oralis
Strep salivarius group (species)
S. salivarius
S. vestibularis
Strep bovis group (characteristics)
Bile esculin positive, cannot survive 6.5% NaCl
Strep bovis group (species)
S. gallolyticus subsp gallolyticus
S. gallolyticus subsp pasteurians
S. infantarius
S. alactolyticus
Nutritionally Variant Streptococci (species)
Abiotrophia
Granulicatella
Nutritionally Variant Streptococci requirements to grow
pyrodoxal or cysteine
Nutritionally Variant Streptococci grow on which agar?
CHOC
Nutritionally Variant Streptococci are usually resistant to which antibiotic?
Penicillin
What test is presumptive for Enterococcus
Bile Esculin (turns BEZ dark brown to black)
Bile esculin positive organisms
Enterococcus S. bovis group Lactococcus Vagococcus B. fragilis
PYR positive GPCs
Enterococcus
S. pyogenes
Staphylococcus (lugdunensis, schleferi, intermedius, haemolyticus)
NVS (Abiotrophia, Granulicatella)
Aerococcus viridans Aliococcus Gemella Globicatella Lactococcus Vagococcus
Enterococcus sp (defining biochemicals)
PYR positive
Bile esculin positive
grow in 6.5% NaCl
Non-enterococci GPCs that are Bile esculin positive
Lactococcus
Vagococcus
Non-enterococci GPCs that grow in NaCl
Leuconostoc
Lactococcus
Glucose fermenters
Enterobacteriaceae
Vibrio
Aeromonas
Pasteurella
Non-glucose fermenters
Pseduomonas
Burkholderia
Stenotrophomonas
Acinetobacter
What member of the Enterobacteriaceae is oxidase positive?
Plesiomonas
Oxidase positive orgs
Pseudomonas aeruginosa
Burkholderia cepacia
Plesiomonas
Aeromonas Vibrio Moraxella Neisseria Bordatella parapertussis Burkholderia pseduomallei Capnocytophaga (dog species) Brucella Campylobacter Helicobacter H-CEK Chromobacterium violaceum Methylobacterium Ochrobactrum Pasteurella Psychrobacter Roseomonas
What does spot indole test for?
The ability to split indole into tryptophan
Indole pos orgs
E. coli Klebsiella oxytoca Klebsiella ornithinolytica Proteus vulgaris Proteus penneri Citrobacter koseri
Cardiobacterium hominis Aeromonas Pasteurella multocida Vibrio Pleisiomonas Proprionibacterium acnes
H2S positive orgs
Salmonella Erysipelothrix (on TSI) Proteus vulgaris Proteus mirabilis Proteus penneri Edwardsiella tarda Citrobacter freundii
Brucella abortus
Campylobacter coli
Aeromonas hydrophila