BACTERIAL BIOCHEMISTRY METABOLISM Flashcards
Study of the vital life processes of microorganisms
- Nutritional needs
- Metabolic pathways
- Reproduction, growth and death
MICROBIAL PHYSIOLOGY
*All living protoplasm contains 6 major chemical elements:
C, H, O, N, P, S
*Combinations of all these elements make up the
macromolecules: carbohydrates, proteins, lipids and
nucleic acids
*To build necessary cellular materials, every organism
requires a source/s of energy, carbon and other nutritional
requirements
Nutritional Requirements
ENERGY SOURCE
light
PHOTOTROPHS
ENERGY SOURCE
chemicals
CHEMOTROPHS
inorganic materials
Chemolitotrophs
organic materials
Chemoorganotrophs
CO2 as their sole source of carbon
Examples: plants, algae and cyanobacteria
AUTOTROPHS
CARBON SOURCE
AUTOTROPHS
HETEROTROPHS
organic compounds
other than CO2
Examples: humans, animals, fungi, bacteria and
protozoa
HETEROTROPHS
use light as energy source and CO2 as carbon source
Plants, algae, cyanobacteria, purple and green sulfur bacteria
PHOTOAUTOTROPHS
use light as energy source and organic compounds aside from CO2 as carbon
source
Purple nonsulfur and green nonsulfur bacteria
PHOTOHETEROTROPHS
Use electrons from reduced inorganic compounds as a source of energy and
they use CO2 as their main source of carbon
Thiobacillus thiooxidans, Nitrosomonas, Nitrobacter, Hydrogenomonas
CHEMOAUTOTROPHS
use chemicals as energy source and organic compounds other than CO2 as carbon
source (all clinically impt bacteria are of this group)
Animals, protozoa, fungi and most bacteria
CHEMOHETEROTOTROPHS
Biochemical reactions or cellular processes for survival and
replication. Involves:
* Break down of organic compounds
* Synthesis of new bacterial parts
BACTERIAL METABOLISM
Energy is generated from these metabolic breakdowns and all
these biochemical reactions are controlled by specific
enzymes
BACTERIAL METABOLISM
Anaerobic process carried by both obligate and facultative
anaerobes; electron acceptor is an organic compound
Fermentation
When fermentation occurs, a mixture of end products is
accumulated like: lactate, butyrate, ethanol, acetoin
Fermentation
In dx micro: fermentation could indicate any type of
utilization (fermentative or oxidative) of a CHO (sugar)
with the resulting production of an acid pH
Fermentation
These pathways break down GLUCOSE to form
PYRUVIC ACID; precursor metabolites are also produced
by these 3 central pathways:
1.Embden-Meyerhof-Parnas (EMP)Pathway
2.Entner-Doudoroff Pathway
3.Pentose Phosphate Shunt
Biochemical Pathways
Fermentation pathways of microbes in the human host:
- Alcoholic fermentation
- Homolactic fermentation
- Heterolactic fermentati0n
- Propionic acid fermentation
- Mixed acid fermentation
- Butanediol fermentation
- Butyric acid fermentation
Anaerobic Utilization of Pyruvic
Acid (Fermentation)
cycle is the most important
pathway for the complete oxidation of a substrate under
aerobic conditions
Krebs/TCA/Citric acid
Krebs/TCA/Citric acid cycle is the most important
pathway for the complete oxidation of a substrate under
aerobic conditions
Aerobic Utlization of Pyruvic acid
Oxidation
In this cycle, pyruvate is oxidized, carbon skeletons are
created, and the e- are passed through the electron
transport chain (ETC) and used to generate energy in the
form of ATP
Aerobic Utlization of Pyruvic acid
Oxidation