Bacteria - Week 2 PBL

Question 1

What is light microscopy?

Answer 1

Using microscopes and bright fields to examine cultures and motility of organisms

Question 2

What is gram staining?

Answer 2

Observe gram pos (stains violet; thick peptidoglycan layer) and neg (stains red; thinner peptidoglycan layer).

Question 3

What is traditional culture?

Answer 3

The cultivation of microorganisms for analysis

Question 4

What are the four stages to PCR?

Answer 4

1) denaturation - heat tube
2) annealing - cool tube, DNA polymerase binds primers and adds nucleotides to template strand
3) extension- new complementary DNA strand
4) electrophoresis analysis

Question 5

What is serology?

Answer 5

Immunoassays for antigen conc measurement

Question 6

5 key features of bacteria:

Answer 6

Long circular DNA
Thick capsule
Flagellum
Binary fission reproduction
Cell wall (peptidoglycan)

Question 7

What is the name of spherical bacteria?
An example and gram type

Answer 7

Cocci
Streptococcus pneumoniae
Gram pos

Question 8

What is the name of rod shaped bacteria?
Example and gram type (x2)

Answer 8

Bacilli
Bacillus cereus
Gram pos

E. coli
Gram neg

Question 9

What is the name for spiral shaped bacteria?
Example and gram type

Answer 9

spirochaetes
Helicobacter pylori
Gram neg

Question 10

What is the name for common shaped bacteria?
Example and gram type

Answer 10

Vibrios
Vibrio cholerae
Gram neg

Question 11

What is the chain of infection and why is it important ?

Answer 11

Its how communicable diseases spread

(Host/infectious agent/reservoir/exit/transmission/ entry)