Bacteria Structure and Characteristics Flashcards

1
Q

Bacterial Classifications

A

1) Structural Components
2) Cell Wall
3) Genetic Make-up

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2
Q

Cocci

A
  • Circular
  • mainly gram positive
  • Streptococci divides forming long chains
  • Staphylococci divides forming grape like structures
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3
Q

Bacilli

A
  • Rod like appearance

- Normally gram negative

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4
Q

Beta hemolytic Bacteria

A
  • complete hemolysis lysing the red blood cells
  • zone of exclusion = bacteria secreting lysin destroying RBCs
  • generally more dangerous than other forms
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5
Q

Gamma Bacteria

A
  • nothing is happening, not secreting anything; therefore no damage to the RBCs
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6
Q

Alpha Bacteria

A
  • hemoglobin is converted to methamoglobin resulting in the color change from red to brown
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7
Q

Structures common to ALL bacteria cells

A
  1. Cell membrane/envelope
  2. Cytoplasm
  3. Ribosomes
  4. One (or few) chromosome
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8
Q

Cell Envelope

A
  • composed of multiple layers
  • Gram + = 2 layers
  • Gram - = 3 layers
  • Acid fast bacteria = 2.5 layers
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9
Q

Gram + bacteria

A
  • inner phospholipid bilayer membrane

- outer peptidoglycan layer consisting of teichoic acid and lipotechoic acid (LTA)

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10
Q

Gram - bacteria

A
  • inner phospholipid bilayer membrane
  • small peptidoglycan layer
  • outer layer composed mainly of lipopolysaccharide (LPS) forming an asymmetrical membrane
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11
Q

Acid Fast bacteria

A
  • inner phospolipid bilayer
  • middle skinny peptidoglycan layer with lipoarabomannans and arabinogalactans
  • half outer layer with mycolic acid head groups that are attached to peptidoglycan layer
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12
Q

Gram + Cell Wall Structure

A
  • thick peptidoglycan cell wall
  • resists lysis by complement
  • teichoic acids and lipotechoic acids
  • TLR 2 recognition of LTA
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13
Q

Gram - Cell Wall Structure

A
  • asymmetric outer membrane
  • thin peptidoglycan layer
  • periplasmic space
  • barrier to non-polar molecules
  • outer membrane porins
  • TLR-4 recognition of LPS
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14
Q

Gram Staining

A
  • Gram positive turns dark violet due to cross linking that occurs changing it from very porous to tight crystal lattice
  • Gram positive turns pink due to the safranin stain because it does not retain the crystal violet stain
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15
Q

Acid Fast Cell Wall Structure

A
  • most similar to gram positive cells - mycobacteria
  • cell wall comprised of fatty acids and waxes
  • lipid components are difficult to stain, but once stained stay stained
  • Mycolic Acid, Wax D present in cell wall
  • TLR-1/2/6 recognition
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16
Q

Ezymes Required for Peptidoglycan Synthesis

A
  1. Transglycosylase

2. Transpeptidase

17
Q

Transglycosylase

A
  • inserts and links new peptidogylcan monomers

- NAG….NAM….NAG….NAM

18
Q

Transpeptidase

A
  • forms the peptide cross links between the rows and layers of peptidoglycans
19
Q

Flagella

A
  • composed of flagellin
  • helical filament that rotates like a screw
  • present on Bacilli and spirochetes but not cocci!
  • Anticlockwise - swimming movement to something good
    Clockwise - tumbling; moving from something bad
20
Q

Monotrichous

A
  • single flagella from one pole

- characteristic of Vibrio

21
Q

Lophotrichous

A
  • multiple flagella from same spot
22
Q

Amphitrichous

A
  • single flagella from each pole
23
Q

Peritrichous

A
  • Many flagella in all directions

- characteristic of E. Coli

24
Q

Pili/Fimbriae

A
  • present in both Gram +/-
  • twitching motility
  • adhesion to surfaces
  • Fimbriated strains of E. Coli agglutinate RBCs
  • Pili help in the transfer of genetic material through facilitating conjugation