Bacteria and Pathogens Flashcards
Tests that identify gram + cocci?
blood agar - hemolysis
coagulase testing
PURPLE
tests to identify gram - rods?
oxidase testing
Lactose testing
PINK
which is gram - and which is +?
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PURPLE - positive
PINK - negative
what a culture report tells and does NOT tell you
Tells - identify bacteria / fungus
NOT - infection vs colonization
which abx to use
define MIC
The MIC number is the lowest concentration (in μg/mL) of an antibiotic that inhibits the growth of a given strain of bacteria.
Gram + cocci in clusters is noted, most likely organism?
staphlyococcus
name some common gram + COCCI
staph
strep
enterococcus
peptostreptococcus (anaerobic)
name some common gram + bacilli
clostridium (anerobic)
acillus anthracis
listeria
corynebacterium
CALC
Name some common Gram - COCCI
neisseria
gonorrhoeae
nerisseria meningitdes
moraxella
Name some common gram - Bacilli (lactose fermenting)
serratia
E. coli
enterobacter
Klebsiella
citobacter
SEEK C
name some common gram - bacilli (non-lactose fermentaing)
pseudomonas
acinetobacter
salmonella
shigella
proteus
camplyobacter
bacteroides
Yersinia
Stenotrophomonas
legionella
PASS PCB YSL
where is staph aerus normall found
skin
nares
respiratory tract
name dx where s. auerus could be pathogenic
cellulitis
abcesses
bone, joint
endocarditis
MRSA/MSSA
where is staphlyococcus epidermidis normally found?
is it virulent?
skin
no - assoc w/ prothetic and device infections
contaminant in blood cultures common
is staph epidermidis coagulase + or -
NEG
what are the sites of infection for HA-MRSA
lungs
GU
blood
surgical sites
what are the sites of infection seen with CA-MRSA
skin/soft tissue
lungs (causeing nectrotizing pneumonia)
younger population
resistance of HA-MRSA
B-lactams
macrolides
clinda
FQs
TMP-SMX (bactrim)
tetracyclines
resistance of CA-MRSA
beta-lactams
macrolides
increased FQs
tetracyclines
examples of alpha hemolytic streptococcus
s. pnuemoniae
strep viridans
examples of beta hemolytic streptococcus
Greou A hemolytic strep (GABHS)
Group b streptococcus
examples of gamma hemolytic streptococcus
enetercoccus
peptostreptococcus
enetercoccus are Gram (Negative / Positive) Cocci, anerobic, Belong to the GROUP ___ Streptococcus
POSITIVE
Group D
where does entercoccus usually colonize?
is it virulent?
human intestine
occasionally vaginal and oral cavity
NO- oppurtunistic pathogen most common UTI from catheterization
enterococcus is intrinsically resistant to a variety of commonly used antimicrobials and has also acquired ____ resistance.
VRE
Define enteric gram -
Enteric means a group of bacteria that live in intestines aka “GUT” GNR (Gram Negative Rods)
Aerobic - Include lactose and non-lactose fermenting organisms
common enteric gram - pathogens
Enterobacteriaciae family:
E. Coli
Proteus
Serratia
Acinetobacter
Citrobacter
Enterobacter
Salmonella
Klebsiella
SPACES KC
hospital acquired infections caused by Enterobacteriaciae.
community acquired?
Late Hospital Acquired or Ventilator assisted pneumonia
complicated UTI
CA- abdominal inf
UIT
H-N-M are gram ____ (anaerobic / aerobic) cocci
Haemophilus (coccobacilli) • Neisseria • Moraxella
NEG aerobic cocci
drug resistance of Enterobacteriaciae
beta lactams
ESBLs
CRE
Common infections of Haemophilus and Moraxella include ______ ______ ______.
Common infections of Neisseria are ______ and ____.
Haemophilus and Moraxella include upper and lower respiratory tract infections
Neisseria are meningitis and STIs
Pseudomonas Aeruginosa is a gram ____, ______ lactose fermenting organism.
NEG
Non-lactose
common infections of pseudomonas aeruginosa?
Late hospital acquired or healthcare associated pneumonia
Wounds
Osteomyelitis
Corneal ulcer (contact lens)
Hot tub folliculitis
identify bacteria causing infection?
what is infection with this pathogen usually associated with?
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psudeomonas aeringusa
associated with hospitals and infections after antibiotics
bacteria that do not stain, remain colorless because they do not have a cell wall and are known to be zoonotic
atypicals
name the atypical pathogens
Chlamydia
Legionella
Mycoplasma
Rickettsia
Spirochete infections (Leptospirosis, Borrelia, Treponem Pallidium)
what type of infections do atypical pathogens usually cause
URI, LRI
STI
Lyme
define empiric therapy
Antibiotics chosen prior to knowing identification and sensitivity of pathogen • Important to understand types of bacteria that are known for causing disease (Previous infections • Local sensitivity)
define abx de-escalation
Narrowing therapy from broad agents
define Antimicrobial Stewardship
Preserving broad-spectrum antibiotics
Limit development of resistance
define nosocomial infection
An infection acquired in a hospital setting or health care facility.
Traditionally >48 hours
Name gram positive bacilli anerobes
and the one cocci
clostridum
actinomyces
lactobacillis
propionibacterium
peptostreptococcus (cocci)
name gram negative anerobes (bacilli and one cocci)
b. fragilis
fusobacterium
porphyromonas
veillonella (cocci)
are anaerobes virulent by themselves?
no (co-infection)
normal flora on skin
staph epidermidis
strep pyrogenes (group A strep)
normal dental flora
streptococcus
peptopstreptococcus
normal oropharynx flora
group A beta hemolytic strep
peptostrepto
normal respiratory flora
strep
staph
haemophilus
neisseria
normal GI flora
enterococcus
clostridium
bacterides
enterobacteriaciae
normal flora of vagina
strep B
lactobacillis
bacteroides
anaerobes
Located on lower extremities/face. Fiery red, painful, raised, demarcated lesions.
Dx?
pathogens that usually cause?
erysipelas
B-hemolytic strep (S. pyogenes)
Non demarcated redness, Sever systemic effects are rare
Dx?
pathogens most likely to cause?
uncomplicated cellulitis
B hemolytic strep + s. auerus
Painful, warm to touch lesions, often purulent
Dx?
pathogens that usually cause?
abscess
s. auerus + MRSA
pathogens respoinsible for ery, cellulitis, abscesses?
ery - B-hemolytic strep (s. pyogenes)
cell - b hemolytic strep + s. auerus
abscess - S. aeurs + MRSA
pathogens likely to cause mouth/tooth infections
Streptococcal species
Peptostreptococcus
other grampositive anaerobes
pathogens most likely to cause conjunctivits
S. aureus
S. pneumoniae
H. influenza
M. catarrhalis
VIRAL!!!
APIC
bilaterial is often allergic
Patient presentation: fever, malaise, sore throat, dysphasia, lymphadenopathy, white patches/pus, rash.
pathogen responsible?
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Group A beta hemolytic Streptococcus
pathogens most likely to cause HCAP or VAP
Pseudomonas aeruginosa
Klebsiella pneumoniae
Acinetobacter species
Methicillin-resistant S. aureus
pathogens responsible for CAP
Streptococcus pneumonia
Mycoplasma pneumonia
Haemophilus influenza
Chlamydia pneumonia
Patients present with dysuria, hematuria, frequency Confusion and malaise in elderly patients.
pathogen likely responsible?
E. coli (70-95%)
Klebsiella
proteus
pathogens of the GI tract
Enterobacteriaciae – E.Coli, Enterococcus, Klebsiella, Bacteroides Fragilis
pathogens most likely to affect the meninges
Strep. Pneumoniae
Neisseria Meningitides
Listeria
most common bacterial pathogens seen w/ otitis media
S.pneumoniae
H. flu
name sterile sites in the body
Cerebrospinal Fluid
Blood
Peritoneum
Synovial fluid
Pleural fluid
Urine *
colonization vs contamination?
Identification of lactobacillus in a vaginal culture
colonization
colonization vs contamination?
Identification of coagulase negative staphylococcus species in a single set of blood cultures
contamination
unintended introduction of a bacterial organism that isnt infectious
Name the ESBL (Extended spectrum beta-lactamase organisms)
SPACE
Serratia
Pseudomonas
Acinetobacter
Citrobacter
Enterobacter
Klebsiella
what are the CRE pathogens?
Carbapenem- resistant enterobacteriaceae
E. Coli
Proteus
Serratia
Acinetobacter
Citrobacter
Enterobacter
Salmonella
Klebsiella
SPACES K
strep viridans is located in the skin and ____ and is most likely responsible for _____ and _____ _____.
mouth
endocarditis, mouth infections
Group B strep is normally located in the vagina and can cause ____ in neonates
meningitis
eneterococcus or strep D is located in the _____ and is responsible for ____, ___, ______ related infections
GI
UTI, GI, catheter-related infections
peptostreptococcus is normally seen in the ____ and causes _____ infections.
mouth
dental
clostridium perfinges causes _______ and _______.
gas gangrene
NF
Listeria is normally found in animals and food products and causes ____, ____, _____.
Meningitis, diarrhea, bacteremia
actinomyces is normally found in the ____ and is responsible for skin and soft tissue infections.
mouth
Enterobacteriaciae are located in GI tract and can cause ____, ____, _____. Klebsiella can cause _____.
Diarrhea, urinary tract infection, food poisoning *Klebsiella- can cause pneumonia
fecal-oral
bacteria located in GI tract that causes Intra-abdominal infections, intraabdominal Abscesses
Bacteroides Fragilis
bacteria that causes infections in immunocompromised hosts.
pseudomonas
diharrhea or bactermia resulting from eating contaminated foods that were likely contaminated by ____
Campylobacter
Legionella causes ______ and ______
Pneumonia, febrile illness
pathogen likely responsible for pharyngitis, otitis media and sinusitis
M. catharralis
pathogen responsible for Urethritis, cervicitis, PID
Neisseria Gonorrhoea
pathogen likely causing meningitis
neisseria meningitis
pathogen endogenous to the respiratory tract that causes respiratory sinusitis, otitis and meningitis
Haemophilus
Chlamydophila pneumoniae usually colonizes and causes
genitals, throat, eyes
UTI, STI, throat
Mycoplasma pneumoniae usually colonized ____ or _____ and causes?
node or throat
CAP
meningitis
Legionella is found in the soil and causes
pneumonia
WHAT IS/ARE THE MOST LIKELY ORGANISMS TO CAUSE COMMUNITY ACQUIRE D PNEUMONIA?
Strep pneumonia
Haemophilus influenza
Mycoplasma pneumonia
Chlaymdia pneumoniae
WHICH OF THE FOLLOWING ORGANISMS IS LIKELY TO PRODUCE AN EXTENDED SPECTRUM BETA LACTAMASE RESISTANCE?
s. aerus
enterococci
psuedomonas
e. coli
e coli
ALL OF THE FOLLOWING ARE THE ORGANISMS ASSOCIATED WITH AN UNCOMPLICATED URINARY TRACT INFECTION EXCEPT?
e. coli
klebsiella
enterococcus
proteus
enterococcus
when culturing blood what are we looking for?
Sepsis
Pneumonia
Endocarditis
Osteomyelitis
Meningitis
when culturing sputum what are we looking for?
infectious etiollgy of pnuemonia
TB
when would we culture CSF?
what specific pathogens may we find?
Septic patient with neurological findings, transverse myelitis
neisseria
strept pnuemonia
H. flu
a throat cultre helps identify what organisms?
Group A strept,
diphtheria
gonococcal
candida
when would we culture stool
food borne illness
C diff
diarrhea
what are we looking for in a urine culture (specifc organisms)
e. coli
klebsiells proteus
when would we culture a wound/abscess
looking for pseudomonas
staph
strept
when would we culture genital tract?
STIs