Bacteria Flashcards
prokaryotic, cell wall, single chromosome, 70S ribsome
bacteria characteristics
stain, shape, oxygen requirements, temperature, others
classification of bacteria
lag, log, stationary and death
bacterial growth phases
number of cells are constant as they prepare for growth; no multiplication
lag growth phase
exponential growth
log growth phase
new growth matches die off rate as nutrients run out of food
stationary growth phase
gram positive (BLUE) or negative (RED)
stain
crystal violet, H2O, iodine, H2O, acetone alcohol wash, safranin, H2O
gram stain
iodine (mordant or fixer) and safranin (counterstain)
mordant and counterstain
coccus (round), bacillus (rod) or vibrio (comma-shaped)
shape
aerobic (grows in O2) or anaerobic (can grow without O2)
oxygen requirement
prefers to live without O2 but has the facility (ability) to live with oxygen
facultative anaerobic
cannot live in the presence of O2
obligate anaerobic
cannot live without oxygen-mycobacteria, bacillus, and pseudomonas
obligate aerobic
needs a very small amount of O2 - helicobacter pylori, treponema pallidum
microaerophilic
thermophile (45-900), mesophile (20-450), or psychrophile (0-200)
temperature preferences
urease, nitrite producing, catalase positive, specific sugar fermentation
other ways of grouping bacteria
klebsiella, proteus, ureaplasma, nocardia, (cryptococcus), helicobacter
urease (urea splitting)
klebsiella, escherichia coli, enterobacter, pseudomonas, proteus, serratia
nitrite-producing
pseudomonas, listeria, escherichia coli, and staphylococcus
catalase - positive
staphylococcus aureus; all other staphylococci and coagulase negative
coagulase - positive coccus
neisseria gonorrhea and neisseria meningitidis
glucose - fermentation coccus
escherichia coli and klebsiella pneumoniae
lactose - fermenting bacilli
neisseria meningitdis and proteus mirabilis
maltose fermenting bacteria
mycoplasma
bacteria without cell walls
chlamydia, rickettsiae, ehrlichia, bartonella, and coxiella
obligate intracellular parasite
borrelia, leptospira, treponema
flexible bacteria (spirochetes)
all bacteria have cell walls except MYCOPLASMA
bacteria rule 1
all cocci are gram + except NEISSERIA
bacteria rule 2
all bacilli are gram - except the baccilus group
bacteria rule 3
bacillus, clostridium, corynebacterium, and listeria
baccilus group
all gram + bacilli are spore forming except corynebacterium and listera
bacteria rule 4
all gram - bacilli are enteric except 8
bacteria rule 5
all UTI causing bacteria are nitrite + except STAPH, SAPROPHYTICUS
bacteria rule 6
gram + spore forming rod causes woolsorter’s dz, malignant pustule
baillus anthracis
gram + spore forming rod, food poisoning from eating reheated fried rice
bacillus cereus
gram - non enteric rod causing cat scratch dz
bartonella henselae
paroxysmal bouts of coughing followed by an inspiratory whoop
bordetella pertussis
spirochete, darkfield microscopy, ixodes vector deer tick, lyme dz
borrelia burgdorferi
gram -, non enteric, causes undulant fever, contaminated milk
brucella abortus
obligate intracellular parasite, causing lung infection in bird keepers
chlamydia psittaci
commonest cause of blindness in Africa, spread by flies
chlamydia trachomatis ABC
urethritis, PID, reiters syndrome: conjunctivitis, arthritis, urethritis
chlamydia trachomatis D-K
lymphogranuloma venereum - painful buboes, sign of the groove
chlamydia trachomatis L1 L2 L3
transmitted by contaminated honey and causes paralysis and diplopia
clostridium botulinum
pseudomembranous enterocolitis
clostridium difficile
deep puncture wound followed by muscle necrosis and gas bubbles
clostridium perfringens
causes lockjaw (trismus), produces exotoxin tetanospasmin
clostridium tetani
gram + rod, grey pseudomembrane in the throat and heat damage
corynebacterium diphtheriae
obligate intracellular parasite, unpasteurized mild, skin rash with Q fever
coxiella burnetti
gram -, enteric catalase + rod causing UTI
enterobacter cloacae
gram -, enteric lactose fermenting, commonest cause of UTI
escherichia coli
O and H types are used to identify pathogenic stains
e coli typing
causes hemolytic uremic syndrome
e coli subtype O157:H7
gram -, non enteric rod, fever from handling infected rabbit skin
francisella tularensis
gram -, urease + rod, causes peptic ulcer, gastritis, stomach cancer
helicobacter pylori
gram -, non enteric rod, pink eye dz
hemophilus aegypti
gram -, non enteric rod, causes chancroid painful yellow genital sore
hemophilus ducreyi
gram -, non enteric rod, pneumonia, otitis, epiglottis, meningitis
hemophilus influenzae
gram -, enteric, lactose fermenting bacillus pneumonia, UTI
klebsiella pneumoniae
gram -, non enteric, lung infection from infected air conditioners
legionella pneumonphila
spirchete, darkfield microscopy, weil’s dz. spread by rats
leptospira interrogans
gram + non spore forming, contaminated milk or cheese, meningitis
listeria monocytogenes
gram resistant, thick waxy wall, ziehl neelsen stain, lowenstein jensen m.
mycobacterium tuberculosis
gram resistant bacteria like cool places nerves and face
mycobacterium leprae
gram - intracellular glucose fermenting coccus urethritis
neisseria gonorrhea
gram - coccus which ferments maltose and glucose meningitis
neisseria meningitidis
gram -, enteric urea splitting bacillus causing UTI
proteus mirabilis
gram -, non enteric bacillus, infected burns with blue green pus
pseudomonas aeruginosa
gram -, enteric, causes rose colored spots and step ladder fever
salmonella typhi
gram - bacillus, produces red pigment
serratia marcescens
gram -, enteric, non lactose fermenting, causes bloody diarrhea
shigella dysenteriae
gram +, catalase and coagulase + coccus, infecting healthy valves
staphylococcus aureus
gram +, catalase +, coagulase - coccus causes UTI
staphylococcus saprophyticus
gram +, catalase -, alpha hemolytic coccus in strips
streptococcus pneumoniae
grame +, catalase -, beta hemolytic coccus, rheumatic fever
streptococcus pyogenes
gram +, catalase -, alpha hemolytic, previously damaged valves
streptococcus viridans
spirochete, darkfield microscopy, painless penile chancre
treponema pallidum
gram - enteric rod - causes severe diarrhea and rice water stool
vibrio cholera
gram - enteric rod - causes food poisoning and necrotizing fasciitis
vibrio vulnificus
gram - enteric rod- causes bubonic and pneumonic plague
yersinia pestis
dz transmitted from animals to humans in a specific occupation
zoonosis
francisella tulanersis
rabbit skinning
brucella abortus
abattoir worker
bacillus anthracis
sheep sharer
vibrio vulnificus
fishermen
bartonella henseiae
cat scratch
leptospira interrogans
rats in cane fields
yersinia pestis
rat fleas
allergic to meat after bit by a tick infected with ehrlichia chaffeensis
lone star tick
dead gram - bacteria, outer wall, heat stable, systemic effects
endotoxin
live gram +, destroyed by heat, antigenic, targets specific tissue
exotoxin
exotoxin which targets the nerves, stops GABA or glycine production
tetanospasmin
exotoxin which targets the acetylcholine receptor a the NMJ
botulinum