Bacteria Flashcards
prokaryotic, cell wall, single chromosome, 70S ribsome
bacteria characteristics
stain, shape, oxygen requirements, temperature, others
classification of bacteria
lag, log, stationary and death
bacterial growth phases
number of cells are constant as they prepare for growth; no multiplication
lag growth phase
exponential growth
log growth phase
new growth matches die off rate as nutrients run out of food
stationary growth phase
gram positive (BLUE) or negative (RED)
stain
crystal violet, H2O, iodine, H2O, acetone alcohol wash, safranin, H2O
gram stain
iodine (mordant or fixer) and safranin (counterstain)
mordant and counterstain
coccus (round), bacillus (rod) or vibrio (comma-shaped)
shape
aerobic (grows in O2) or anaerobic (can grow without O2)
oxygen requirement
prefers to live without O2 but has the facility (ability) to live with oxygen
facultative anaerobic
cannot live in the presence of O2
obligate anaerobic
cannot live without oxygen-mycobacteria, bacillus, and pseudomonas
obligate aerobic
needs a very small amount of O2 - helicobacter pylori, treponema pallidum
microaerophilic
thermophile (45-900), mesophile (20-450), or psychrophile (0-200)
temperature preferences
urease, nitrite producing, catalase positive, specific sugar fermentation
other ways of grouping bacteria
klebsiella, proteus, ureaplasma, nocardia, (cryptococcus), helicobacter
urease (urea splitting)
klebsiella, escherichia coli, enterobacter, pseudomonas, proteus, serratia
nitrite-producing
pseudomonas, listeria, escherichia coli, and staphylococcus
catalase - positive
staphylococcus aureus; all other staphylococci and coagulase negative
coagulase - positive coccus
neisseria gonorrhea and neisseria meningitidis
glucose - fermentation coccus
escherichia coli and klebsiella pneumoniae
lactose - fermenting bacilli
neisseria meningitdis and proteus mirabilis
maltose fermenting bacteria
mycoplasma
bacteria without cell walls
chlamydia, rickettsiae, ehrlichia, bartonella, and coxiella
obligate intracellular parasite
borrelia, leptospira, treponema
flexible bacteria (spirochetes)
all bacteria have cell walls except MYCOPLASMA
bacteria rule 1
all cocci are gram + except NEISSERIA
bacteria rule 2
all bacilli are gram - except the baccilus group
bacteria rule 3
bacillus, clostridium, corynebacterium, and listeria
baccilus group
all gram + bacilli are spore forming except corynebacterium and listera
bacteria rule 4
all gram - bacilli are enteric except 8
bacteria rule 5
all UTI causing bacteria are nitrite + except STAPH, SAPROPHYTICUS
bacteria rule 6
gram + spore forming rod causes woolsorter’s dz, malignant pustule
baillus anthracis
gram + spore forming rod, food poisoning from eating reheated fried rice
bacillus cereus
gram - non enteric rod causing cat scratch dz
bartonella henselae