bacteria Flashcards
Classifying Prokaryotes
A. Eubacteria
1. e-coli lives in the human gut
2. cells walls contain peptidoglycan - a
carbohydrate structure that gives shape to
the cell.
B. Archaebacteria
1. Chemically different to eubacteria
2. lacks peptidoglycon
3. DNA sequences is more like eukaryotes
than eubacteria
4. live in harsh environments
a. Oxygen free
b. hot springs
B. Archaebacteria
- Chemically different to eubacteria
- lacks peptidoglycon
- DNA sequences is more like eukaryotes
than eubacteria - live in harsh environments
a. Oxygen free
b. hot springs
A. Eubacteria
- e-coli lives in the human gut
- cells walls contain peptidoglycan - a
carbohydrate structure that gives shape to
the cell.
B. Archaebacteria - Chemically different to eubacteria
- lacks peptidoglycon
- DNA sequences is more like eukaryotes
than eubacteria - live in harsh environments
a. Oxygen free
b. hot springs
Identifying Prokaryotes
A. Shape
1. Rods - Bacilli - botulism
2. Spiral - Cocci - Staph infection
3. Corkscrew - spirilla - strep infection
B. Cell walls
1. Thick peptidoglycan stains violet
2. Thin peptidoglycan stains red to pink
C. Movement
1. Some do not move
2. Whip-like flagella
Metabolic Diversity - How prokaryotes obtain
energy
A. Heterotrophs - energy form other organisms
1. Chemoheterotrophs - breaks down food to
make energy ( eats food)
2. Photoheterotrophs - uses sunlight to make
food (Euglena - single celled
organism that has a whip-like
tail to move and has
chloroplast to capture sun for
energy)
B. Autotrophs -
1. chemoautotrophs - uses chemical
reactions to make energy - uses
hydrogen sulfide gas near ocean vents
2. Photoautotrophs - uses sunlight to make
energy - cyanobacteria
C. Releasing energy - All organisms need
energy
1. Obligate aerobes - organisms that need a
constant supply of Oxygen to live
Micro-bacterium - tuberculosis
2. Obligate anaerobes - organisms not
requiring oxygen to live
clostridium botulinum
3. Facultative anaerobes - Able to function
different ways. Does not require oxygen,
but is not killed in the presence of oxygen
- can live anywhere e.coli
Growth and Reproduction of bacteria
bacteria growth can
be exponential. Bacteria growth is kept in
check because of available food and production
of wastes.
A. Binary fission - no exchange of genetic
information - simple dividing in half
Conjugation - exchange of DNA between two
cells, then divide in half - Increased
genetic diversity
Spore formation - allows bacteria to survive
harsh environments which would otherwise
kill them. Endospore protects bacteria’s DNA
Importance of bacteria
A. Decomposers - All life needs to break down
it’s component parts after death. If not, new
life would not exist.
1. Recycle nutrients - to maintain ecological
balance
2. Breaks down complex compounds in
sewage
B. Nitrogen fixer - Bacteria fixes nitrogen so it
can be used by plants to maintain growth -
3
(NH ) Rhizobium in nodes of legumes -
nitrogen fixation
Bacterial disease in humans
- Louis Pasteur showed that bacteria can
cause disease - Destroys cells two ways
a. Attacks cells directly to get cell’s
foods
b. Releases toxins that travel through
body that can cause death, breathing
problems, heart failure, paralysis. - Preventing bacterial disease
a. Stimulating bodies immune system -
vaccines are weakened or killed
pathogens
b. Antibodies - drugs that destroy
bacteria - Penicillin and tetracycline
Bacterial Disease in animals - Anthrax
A. Found in sheep
B. Tough, resistant spores ideal for biological
warfar
Controlling Bacterial
Most bacteria are harmless
some are not.
A. Sterilization by heat - bacteria cannot survive
when exposed to high temperatures for a long
time.
B. Disinfectants - chemical solutions that kill
pathogens
C. Food storage and processing
1. Refrigerator - cold, short period
2. Canning - not indefinitely.
clostridium botulinum
causes botulism
affects nerves
and transmitted by improperly preserved foods
vibrio cholerae
causes cholera
affects intestine
transmitted by contaminated water
streptoccocus mutans, sanguis and salivarius
causes dental caries (tooth decay)
affects the teeth
transmitted by bacteria that enter the mouth from the environment
neisseria gonorrhoeae
causes gonorrhea, affects the urethra, fallopian tubes and epididymis
transmitted person to oerson by sexual contact
borrelia burgorferi
causes lyme diseases, affects skin, joints, heart
transmitted by tick bites
rickettsia ricketsii
causes rocky mountain spotted fever, affects blood and skin. spread by tick bites
salmonella
causes salmonella food poisoning, affects intestine. spread contaminated food sand water
streptoccoccus pyrogenes
causes strep throat, affects the upper respiratory tract, blood and skin. spread by person to person sneezes, coughs, or direct contact
clostridium tetani
tetanus, affects nerves at synapses. and spread by contaminated wounds
mycobacterium tuberculosis
causes tuberculosis. affects the lung, bones and other organs. spread by oerson to person by coughs
What is the primary stain used in the Gram staining procedure?
Crystal violet
Crystal violet stains both gram-positive and gram-negative bacteria purple.
What is the function of Gram’s iodine in the Gram staining procedure?
It acts as a mordant to form an insoluble crystal violet-iodine complex
This complex enhances the retention of the primary stain in bacteria.