Bacte & Mycoviro Flashcards
Average bacterial size
0.4 - 2 micra
Cell wall deficient bacteria
Mycoplasma
What is used to demonstrate Mycoplasma?
A) India ink
B) Acridine orange
C) Hucker’s modification
D) Wayson’s stain
Acridine orange
Spore forming bacterias
Clostridium and Bacillus
Smallest living organism
T-strain mycoplasma
What is the longest bacteria in terms of length?
Borrelia
A part of a bacteria that is used for attachment to host cells
Common pili / somatic pili
Grays & leifson is _______
Used for flagellar staining
What is the capsule of P. Aeruginosa made out of?
A) Polysaccharide D-glutamate
B) Hyaluronic Acid
C) Alginate
D) Peptidoglycan
Alginate
What is the capsule of B. anthrasis made out of?
A) Polysaccharide D-glutamic acid
B) Hyaluronic Acid
C) Polysaccharide D-glutamate
D) Peptidoglycan
Polysaccharide D-glutamic acid
Babes ernst bodies are found in _______
C. diptheriae
All of the following stains are used for staining babes ernst bodies except for:
A) Loeffler’s MB
B) Gram’s
C) Albert’s
D) Neisser’s
E) None of the above
Gram’s
Bacteria with terminal swollen spore:
C. tetani
Bacteria with terminal swollen spore:
C. tetani
Bacteria with central spore:
B. anthracis
Bacteria with subterminal spore:
C. botulinum
Aerobes need ___% O2 and ___% CO2
21%; 0.03%
Microaerophiles require ___% O2 in order to survive
5-10%
Campylobacter spp. and H. pylori are classified as:
Capnophilic & microaerophilic
Capnophilic means that they require ___% CO2
5-10%
All Vibrios are halophilic except for:
V. cholerae & V. mimicus
Which of the following is considered as halotolerant?
A) S. aureus
B) V. cholerae
C) V. vulnificus
D) E. coli
S. aureus
What is the color of the spore when stained by Schaeffer & Fulton?
Green
Bacteria with twitching motility
Kingella kingae
Bacteria with darting motility
Campylobacter
Bacteria with corkscrew motility
Spiral organisms
Bacteria with shooting star motility
Vibrio cholerae
This is also known as chemical sterilants
Biocides
HEPA filters can remove objects larger than ___
0.3 u
Hospitals use ____ to disinfect blood spillage
Phenol
What is the best disinfectant for blood spillage?
10% sodium hypochlorite
What can be used as a substitute for household bleach?
Vinegar
Which anticoagulants should not be used for bacterial culture?
EDTA & citrate
CSF can be stored at ________ for 6 hours
35-37 C
When CSF is collected from a shunt, what is added?
Thioglycollate
What is the major throat pathogen?
Streptococcus pyogenes
What is the major throat flora?
Viridans strep
What is the method of collection for the detection of Bordetella pertussis?
Nasopharengeal swab
Nasopharengeal swab is used to detect the ______ state of N. meningitidis
carrier
Leptospira is not cultured because:
It takes 8 weeks to grow; patient could have died already
What type of swab should never be used for the collection of viruses?
Calcium alginate
What is the gold standard for the decontamination and digestion of sputum for the detection of Mycobacterium
NALC + NAOH
JEMBEC transport media is used for:
Neisseria
Number of sputum sample to be collected according to the DOH
2 samples in 1 day
Amies transport media is used for:
Respiratory specimens
A staining technique wherein the bacteria may appear colorless
Negative staining
What stain is used to demonstrate the safety pin appearance of Yersinia enterocolitica?
Wayson’s stain, and methylene blue
In Hucker’s modification, crystal violet is added with ______ to stain fungi.
Ammonium oxalate
In AF staining, Fite Farco method is used for _______
M. leprae
In staining acid fast organisms, which is best used in tissues?
Kinyoun’s
In staining acid fast organisms, which is best used for DSSM?
Ziehl Neelsen
How many antibiotic disks can be placed on a 150mm plate?
No more than 12 disks
How many antibiotic disks can be placed on a 100mm plate?
No more than 5 disks
Penicillin, Cephalosporine, and carbapenems are examples of what type of antibiotics?
Beta-lactams (Cell inhibitors)
The lowest concentration that inhibited bacterial growth
Minimum Inhibitory Concentration
Lowest concentration of antibiotic that can kill bacteria
Minimum Bactericidal Concentration
The test that detects inducible clindamycin resistance among the strains of S. aureus
D-test
A quantitative AST using a strip of single antibiotic of different concentration
E-test
Serum Bactericidal test is also known as:
Schlicter test
A clover-leaf-like pattern of zone of inhibition indicates:
Positive result for carbapenemase producing organisms
Brucella, Bordetella, and Pseudomonas are examples of what type of bacteria?
Strict aerobes
In the gaspak jar, what part of it maintains the anaerobiasis?
Palladium pellets
Candle jars are usually used for what organism/s?
Neisseria; Microaerophiles
Media that enhances growth and increases the yield
Enrichment media
The type of media that contains inhibitor/s
Selective media
Alkaline peptone water is used to grow what organism?
Vibrio spp.
Selenite broth is usually used for what bacteria?
Salmonella & Gram (-) bacilli
Middlebrook agar is used for growing:
Mycobacterium
Thayer Martin, Modified Thayer Marting, Martin Lewis, New York City agar, and GC Lect medium are:
Selective for Neisseria
What is the action of vancomycin in the isolation of Neisseria?
Inhibits Gram (+)
What is the aciton of Nystatin in the isolation of Neisseria?
Inhibits fungi
What is the action of colistin in the isolation of Neisseria?
Inhibits Gram (-)
What does the trimetophrim lactate in Modified Thayer Martin do?
Prevents swarming
Human blood bilayer tween is used for:
Gardnerella vaginalis
Bismuth Sulfate Agar is usually used for:
Salmonella
What is the best agar to be used in the isolation of Bordetella?
Regan Lowe
Columbia Colistin Nalidixic Acid is selective for what type of bacteria?
Gram (+) cocci
