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Bacte & Mycoviro Flashcards

1
Q

Average bacterial size

A

0.4 - 2 micra

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2
Q

Cell wall deficient bacteria

A

Mycoplasma

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3
Q

What is used to demonstrate Mycoplasma?

A) India ink
B) Acridine orange
C) Hucker’s modification
D) Wayson’s stain

A

Acridine orange

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4
Q

Spore forming bacterias

A

Clostridium and Bacillus

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5
Q

Smallest living organism

A

T-strain mycoplasma

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6
Q

What is the longest bacteria in terms of length?

A

Borrelia

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7
Q

A part of a bacteria that is used for attachment to host cells

A

Common pili / somatic pili

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8
Q

Grays & leifson is _______

A

Used for flagellar staining

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9
Q

What is the capsule of P. Aeruginosa made out of?

A) Polysaccharide D-glutamate
B) Hyaluronic Acid
C) Alginate
D) Peptidoglycan

A

Alginate

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10
Q

What is the capsule of B. anthrasis made out of?

A) Polysaccharide D-glutamic acid
B) Hyaluronic Acid
C) Polysaccharide D-glutamate
D) Peptidoglycan

A

Polysaccharide D-glutamic acid

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11
Q

Babes ernst bodies are found in _______

A

C. diptheriae

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12
Q

All of the following stains are used for staining babes ernst bodies except for:

A) Loeffler’s MB
B) Gram’s
C) Albert’s
D) Neisser’s
E) None of the above

A

Gram’s

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13
Q

Bacteria with terminal swollen spore:

A

C. tetani

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14
Q

Bacteria with terminal swollen spore:

A

C. tetani

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15
Q

Bacteria with central spore:

A

B. anthracis

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16
Q

Bacteria with subterminal spore:

A

C. botulinum

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17
Q

Aerobes need ___% O2 and ___% CO2

A

21%; 0.03%

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18
Q

Microaerophiles require ___% O2 in order to survive

A

5-10%

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19
Q

Campylobacter spp. and H. pylori are classified as:

A

Capnophilic & microaerophilic

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20
Q

Capnophilic means that they require ___% CO2

A

5-10%

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21
Q

All Vibrios are halophilic except for:

A

V. cholerae & V. mimicus

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22
Q

Which of the following is considered as halotolerant?

A) S. aureus
B) V. cholerae
C) V. vulnificus
D) E. coli

A

S. aureus

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23
Q

What is the color of the spore when stained by Schaeffer & Fulton?

A

Green

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24
Q

Bacteria with twitching motility

A

Kingella kingae

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25
Bacteria with darting motility
Campylobacter
26
Bacteria with corkscrew motility
Spiral organisms
27
Bacteria with shooting star motility
Vibrio cholerae
28
This is also known as chemical sterilants
Biocides
29
HEPA filters can remove objects larger than ___
0.3 u
30
Hospitals use ____ to disinfect blood spillage
Phenol
31
What is the best disinfectant for blood spillage?
10% sodium hypochlorite
32
What can be used as a substitute for household bleach?
Vinegar
33
Which anticoagulants should not be used for bacterial culture?
EDTA & citrate
34
CSF can be stored at ________ for 6 hours
35-37 C
35
When CSF is collected from a shunt, what is added?
Thioglycollate
36
What is the major throat pathogen?
Streptococcus pyogenes
37
What is the major throat flora?
Viridans strep
38
What is the method of collection for the detection of Bordetella pertussis?
Nasopharengeal swab
39
Nasopharengeal swab is used to detect the ______ state of N. meningitidis
carrier
40
Leptospira is not cultured because:
It takes 8 weeks to grow; patient could have died already
41
What type of swab should never be used for the collection of viruses?
Calcium alginate
42
What is the gold standard for the decontamination and digestion of sputum for the detection of Mycobacterium
NALC + NAOH
43
JEMBEC transport media is used for:
Neisseria
43
Number of sputum sample to be collected according to the DOH
2 samples in 1 day
43
Amies transport media is used for:
Respiratory specimens
43
A staining technique wherein the bacteria may appear colorless
Negative staining
44
What stain is used to demonstrate the safety pin appearance of Yersinia enterocolitica?
Wayson's stain, and methylene blue
45
In Hucker's modification, crystal violet is added with ______ to stain fungi.
Ammonium oxalate
45
In AF staining, Fite Farco method is used for _______
M. leprae
45
In staining acid fast organisms, which is best used in tissues?
Kinyoun's
45
In staining acid fast organisms, which is best used for DSSM?
Ziehl Neelsen
46
How many antibiotic disks can be placed on a 150mm plate?
No more than 12 disks
46
How many antibiotic disks can be placed on a 100mm plate?
No more than 5 disks
46
Penicillin, Cephalosporine, and carbapenems are examples of what type of antibiotics?
Beta-lactams (Cell inhibitors)
46
The lowest concentration that inhibited bacterial growth
Minimum Inhibitory Concentration
46
Lowest concentration of antibiotic that can kill bacteria
Minimum Bactericidal Concentration
46
The test that detects inducible clindamycin resistance among the strains of S. aureus
D-test
46
A quantitative AST using a strip of single antibiotic of different concentration
E-test
46
Serum Bactericidal test is also known as:
Schlicter test
46
A clover-leaf-like pattern of zone of inhibition indicates:
Positive result for carbapenemase producing organisms
46
Brucella, Bordetella, and Pseudomonas are examples of what type of bacteria?
Strict aerobes
46
In the gaspak jar, what part of it maintains the anaerobiasis?
Palladium pellets
46
Candle jars are usually used for what organism/s?
Neisseria; Microaerophiles
46
Media that enhances growth and increases the yield
Enrichment media
47
The type of media that contains inhibitor/s
Selective media
48
Alkaline peptone water is used to grow what organism?
Vibrio spp.
49
Selenite broth is usually used for what bacteria?
Salmonella & Gram (-) bacilli
50
Middlebrook agar is used for growing:
Mycobacterium
51
Thayer Martin, Modified Thayer Marting, Martin Lewis, New York City agar, and GC Lect medium are:
Selective for Neisseria
52
What is the action of vancomycin in the isolation of Neisseria?
Inhibits Gram (+)
53
What is the aciton of Nystatin in the isolation of Neisseria?
Inhibits fungi
54
What is the action of colistin in the isolation of Neisseria?
Inhibits Gram (-)
55
What does the trimetophrim lactate in Modified Thayer Martin do?
Prevents swarming
56
Human blood bilayer tween is used for:
Gardnerella vaginalis
57
Bismuth Sulfate Agar is usually used for:
Salmonella
58
What is the best agar to be used in the isolation of Bordetella?
Regan Lowe
59
Columbia Colistin Nalidixic Acid is selective for what type of bacteria?
Gram (+) cocci
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MTAP 2 (1 decks)

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