Bacte Must Knows Flashcards
Inducible resistance to Clindamycin
D Test
Detection of Carbapenemase producers
Modified Hodge Test
Positive MHT MODIFIED HODGE TEST
Clover leaf like Indention
MALDITOF-MS stands for
Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry
Detected microbiral identification in minutes from isolated colonies
MALDITOF-MS
Codes for oxacillin resistance in Staphylococcus
mecA gene
MecA gene product
PBP2a
Most accurate detection of mecA mediated Oxacillin
Cefoxitin
Detects susceptibility of all Staph
Spp to Vancomycin
MIC Broth Dilution Test
Pathogen associated with Colorectal CA and Adenoma
Streptococcus bovis/Group D Enterococcus
Leucine Aminopeptidase reaction of Leuconostoc spo
Negative
Pathogen that causes endocarditis and meningitis. catalase negative gram positive cocci in clusters
Gemella spp
Bacteria appearing Gram positive but just Difficult to decolorize. Grows on MAC, Oxidase negative
Acinetobacter
Endotoxins from Gram Negative bacteria causes
Septic Shock
S. Aureus and S pyogenes causes what toxin/dse
Toxic Shock Syndrome
MUG Test positive organism
E. Coli except O157:H7
Intestinal pathogen
LDC (+) ONPG (-) KCN (-)
Salmonella
Intestinal microflora
LDC (-) ONPG (+) KCN (-)
Citrobacter
Antibiotic that inhibits ESBL/Confirmatory for ESBL
Clavulanic acid
Nonmotile at 37•C BUT Motile at Room temp 25-30 C
Yersinia
Phenyalanine Deaminase positive
PPM
Proteus
Providencia
Morganella
PAD differentiates PPM from
Enterobacteriaceae
Positive PAD test
Green slant/fluid
Positive MUG test
Electric blue fluorescence
Tests that differentiates Morganella from Providencia
Gas citrate ODC
M + - +
P - + -
A bacteria that FERMENTS various sugars with or without gas
Aeromonas
Test to differentiate P. Aeruginosa from Aeromonas
SPOT INDOLE TEST
Aeromonas Spot Indole test result
Positive (blue or blue green color on filter paper after 20 secs
Respiratory infx in Px with Cystic Fibrosis
P. Aeruginosa
B. Cepacia
S. Maltophilia
Phenomenon that helps recognize Haemophilus spp that require V factor
Satellitism
V factor is also known as
Nicotinamide Adenine Dinucleotide (NAD)
or
Coenzyme A
Organisms where Haemophilus spp obtain X factor
S.aureus
S.pneumoniae
Neisseria spp
X factor is also known as
Hemin
Animal bite/scratch
Pasteurella
Whooping cough sample requirement
Nasopharyngeal swab
Prefered culture medium for B.pertussis
Regan-Lowe/Charcoal Cephalexin medium
Prefered culture medium for Legionella
BCYE Buffered Charcoal Yeast Extract
Legionella sample requirement
Sputum, Bronchial washing
Nugent scoring is used for
Bacterial vaginosis
B.fragilis culture medium
Bacteroides Bile Esculin Agar (BBE Agar)
-black colonies
Anaerobic blood agar components
Vitamin K
Hemin
Sheeps blood
Test that determines Bacillus anthracis
Direct fluorescence Antibody
Direct fluorescence Antibody detects presence of
Cell wall polysaccharide
Capsule antigen
String of pearls
Bacillus anthracis
Most common cause of gas gangrene
Clostridium perfringens
Used as Lecithinase positive control
C.perfringens
RESISTANT to most antibiotics except Vancomycin
Corynebacterium Jeikeum
Neonatal meningitis
Listeria monocytogenes
Tumbling motility, umbrella shape
L.monocytogenes
The only GRAM + Bacillus that is H2S POSITIVE
Erysiphilothrix rusiopathae
Bottle brush/test tube brush appearance
E.rusiopathae
Butcher’s dse
E.rusiopathae
Battey bacillus
Mycobacterium avium complex (MAC)
Blood test for TB Infection
IGRAS
Interferon Gamma Release Assays
Can prior BCG vax cause a false positive result in IGRAS?
No
Result same day AFB smear was read
Molecular test/NAA
Nucleic Acid Amplification test
Nucleic Acid Amplification sample requirement
Respiratory specimen
Molar tooth colonies
Actinomyces
Sulfur granules in tissues
Actinomyces
Primary atypical pneumonia or Walking pneumonia
Mycoplasma pneumoniae
Gold standard for Rickettsial antibodies
IFA
Indirect fluorescent Assay
From fungal cell wall that fluoresce when exposed to UV
Calcofluor white
culture that preserves easily disrupted conidial structures
Slide culture
Olive oil loving fungi
Malassezia furfur
Tinea /pityriasis vesicolor
Malassezia furfur
Spaghetti meatball appearance
Malassezia furfur
Athlete’s foot
Trichopyton mentagrophytes
Most common dermatophytes to infect humans
T.rubrum
Haur baiting test positive result
V shaped penetration of hair shaft
Hair baiting test differentiates
T. Mentagrophytes +
T. Rubrum -
Rose gardener’s dse
Sporothrix schenkii
S.schenkii at room temp
MOLD
S.schenkii at 37C
YEAST
Cigar shaped
Viral transport
Reuires storage in ice
Media with serum albumin or gelatin, antibiotics
Method to detect HPV from clinical spx
Hybrid Capture method
Flu viruses are reviewed every_______
EACH YEAR
Brucella culture medium
Castañeda bottles
Cysteine and cystine requirement for growth
-Blood glucose cysteine medium
Francisella tularensis
Stain in observing bipolar bodies
Wayson stain
Bipolar bodies seen in
Yersinia pestis