Bacte

Question 1

BAP ingredients

Answer 1

intact sheep RBC
or type O human blood

Question 2

BAP use

Answer 2

primary plating and subculturing most bacterial isolates

Question 3

MAC ingredients

Answer 3

Lactose
Low conc. bile salts - inhibit gram pos

Question 4

MAC use

Answer 4

selective and differential agar for gram neg
also used as primary plating and subculturing

Question 5

CAP ingredients

Answer 5

hemolyzed sheep RBC
hgb
NAD

Question 6

CAP use

Answer 6

for primary plating and subculting fastidious bacteria such as haemophilus and neisseria

Question 7

TCBS ingredients

Answer 7

sodium thiosulfate
sodium citrate
ox bile
sucrose
peptone

Question 8

TCBS use

Answer 8

isolate vibrio spp

Question 9

SSA ingredients

Answer 9

lactose
bile salts
beef extract
peptone

Question 10

SSA use

Answer 10

isolate Salmonella and Shigella

Question 11

BHI broth ingredient

Answer 11

proteose peptone
dextrose
calf brain and beef heart infusions

Question 12

BHI broth use

Answer 12

liquid medium for cultivation of variety of fastidious and nonfastidious microorg

Question 13

machines for culture bottles

Answer 13

AUTOBIO BC 120 - optical detection system
BD BACTEC FX - detects CO2 via fluorescence

Question 14

Vitek 2 description

Answer 14

performs ID and AST
15 mins processing time
50 cards (10 slots per carrier)

Question 15

principle of bacteria ID using vitek

Answer 15

advanced colorimetry

Question 16

principle of bacteria AST using vitek

Answer 16

broth microdilution MIC technique

Question 17

McFarland Turbidity range for GN and GP

Answer 17

0.50 - 0.63

Question 18

McFarland Turbidity range for YST and BCL

Answer 18

1.80 - 2.20

Question 19

0.5 McFarland Standard is equivalent to

Answer 19

1.5 x 10^8 CFU/mL