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bacte mtap > BACTE > Flashcards

BACTE Flashcards

1
Q

average size of bacteria

A

0.4-2.0 um

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2
Q

no true nucleus

A

prokaryotes

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3
Q

what acid content does spores have to make it resistant?

A

dipicolinic

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4
Q

cell membrane with sterols except

A

genus mycoplasma

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5
Q

primary dye of schaeffer and fulton

A

malchite green

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6
Q

what inclusion bodies does vibrio cholerae have

A

shooting star motility

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7
Q

manner of collection in urine specimen

A

mid stream clean catch

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8
Q

stage of bacterial growth where spores starts to develop

A

plateau/stationary phase

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9
Q

blood-broth ratio

A

1:10

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10
Q

low concentration of oxygen required

A

microaerophilic

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11
Q

2 indicators of TCBS

A

bromthymol blue and thymol blue

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12
Q

anticoagulant not used for culture

A

EDTA

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13
Q

used to support growth of the desired organism while inhibiting growth of others (inhibitor)

A

selective media

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14
Q

used to enhance bacterial growth (vitamins and nutrients)

A

enrichment media

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15
Q

a device that encloses a workplace

A

biosafety cabinet

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16
Q

BSC class that exhaust air outside the building

A

BSC CLASS IIB

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17
Q

poses extreme risk to laboratory personnel

A

BSL-4

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18
Q

destruction and removal of all forms of microbial life including the spores

A

sterilization

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19
Q

not killed by autoclaving

A

prions

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20
Q

to eliminate prions, extended steam sterilization in conjugation with______ is needed

A

1M sodium of hypochlorite

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21
Q

thickening through evaporation

A

inspissation

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22
Q

biological indicator of autoclave

A

geo bacillus stearrothermophilus

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23
Q

biological indicator of ionizing radiation

A

bacillus pumilus

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24
Q

dilution for porous surface

A

1:10

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25
substitute for sodium hypochlorite
vinegar
26
burning of infectious material into ashes
incineration
27
to treat patient’s blood & body fluid as potentially infectious
standard precaution
28
hiss and anthony are special stains for
capsular stain or capsule stain
29
mordant of gram staining
iodine
30
only the background is stained, organism will stain colorless
indirect/relief/negative
31
gram staining technique for fungi
huckers modification
32
aka hot method; best method for DSSM
zeihl neelsen
33
counterstain for acid fast stain
methylene blue/malachite green
34
size of AFB smear
2x3 cm
35
modification of acid fast stain used to demonstrate m. leprae
fite-faraco
36
can inhibit bacterial growth
bacteriostatic
37
drug which can be used as treatment for UTI; inhibits bacterial enzymes
nitrofurantoin
38
manner of streaking using sterile cotton swab in kirby bauer disk diffusion method
overlap streaking method
39
size of filter paper in kirby bauer test
6mm
40
inoculum size of kirby bauer disk
1.5x10^8 CFU/ml
41
substrate of beta lactamase test
nitrocefin
42
encoded by the gene mec A
penicillin binding protein 2a
43
automation program for bacterial identification and susceptibility
vitek
44
same as vitek but faster
maldi tof
45
positive result of chromogenic agar in mrsa detection
rose/mauve colored colony
46
detect inducible clindamycin resistance among strains of s. aureus
d-test
47
in penicillin resistance, greater than 20mm means
sensitive
48
positive result is clover leaf like pattern of zoi
modified hodge test
49
used to measure zone of inhibition
ruler or caliper
50
carried out to determine susceptibility or resistance of organisms to antimicrobial agents
antibiotic susceptibility testing
51
uses tergitol as wetting agent
kinyoun’s
52
most critical step in gram staining
decolorizer
53
quality control for gaspak jar
check every use
54
tincture of iodine
iodine + 70% alcohol
55
removes pathogens in food
pasteurization
56
121 deg c for 30 mins at 15 psi
for contaminated microbial materials
57
examples of this media are CAP BAP HuBA HoBA SBA
enriched media
58
in sputum specimen, poor collection has a presence of
alveolar macrophage and columnar cells
59
in the stages of bacterial growth, it is a phase where this happens because of unfavorable conditions
death phase/phase of decline
60
giving a safety pin appearance and has bipolar bodies
y. pestis

bacte mtap (1 decks)

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