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bacte mtap > BACTE > Flashcards

BACTE Flashcards

1
Q

average size of bacteria

A

0.4-2.0 um

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2
Q

no true nucleus

A

prokaryotes

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3
Q

what acid content does spores have to make it resistant?

A

dipicolinic

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4
Q

cell membrane with sterols except

A

genus mycoplasma

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5
Q

primary dye of schaeffer and fulton

A

malchite green

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6
Q

what inclusion bodies does vibrio cholerae have

A

shooting star motility

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7
Q

manner of collection in urine specimen

A

mid stream clean catch

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8
Q

stage of bacterial growth where spores starts to develop

A

plateau/stationary phase

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9
Q

blood-broth ratio

A

1:10

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10
Q

low concentration of oxygen required

A

microaerophilic

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11
Q

2 indicators of TCBS

A

bromthymol blue and thymol blue

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12
Q

anticoagulant not used for culture

A

EDTA

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13
Q

used to support growth of the desired organism while inhibiting growth of others (inhibitor)

A

selective media

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14
Q

used to enhance bacterial growth (vitamins and nutrients)

A

enrichment media

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15
Q

a device that encloses a workplace

A

biosafety cabinet

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16
Q

BSC class that exhaust air outside the building

A

BSC CLASS IIB

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17
Q

poses extreme risk to laboratory personnel

A

BSL-4

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18
Q

destruction and removal of all forms of microbial life including the spores

A

sterilization

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19
Q

not killed by autoclaving

A

prions

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20
Q

to eliminate prions, extended steam sterilization in conjugation with______ is needed

A

1M sodium of hypochlorite

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21
Q

thickening through evaporation

A

inspissation

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22
Q

biological indicator of autoclave

A

geo bacillus stearrothermophilus

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23
Q

biological indicator of ionizing radiation

A

bacillus pumilus

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24
Q

dilution for porous surface

A

1:10

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25
Q

substitute for sodium hypochlorite

A

vinegar

26
Q

burning of infectious material into ashes

A

incineration

27
Q

to treat patient’s blood & body fluid as potentially infectious

A

standard precaution

28
Q

hiss and anthony are special stains for

A

capsular stain or capsule stain

29
Q

mordant of gram staining

A

iodine

30
Q

only the background is stained, organism will stain colorless

A

indirect/relief/negative

31
Q

gram staining technique for fungi

A

huckers modification

32
Q

aka hot method; best method for DSSM

A

zeihl neelsen

33
Q

counterstain for acid fast stain

A

methylene blue/malachite green

34
Q

size of AFB smear

A

2x3 cm

35
Q

modification of acid fast stain used to demonstrate m. leprae

A

fite-faraco

36
Q

can inhibit bacterial growth

A

bacteriostatic

37
Q

drug which can be used as treatment for UTI; inhibits bacterial enzymes

A

nitrofurantoin

38
Q

manner of streaking using sterile cotton swab in kirby bauer disk diffusion method

A

overlap streaking method

39
Q

size of filter paper in kirby bauer test

A

6mm

40
Q

inoculum size of kirby bauer disk

A

1.5x10^8 CFU/ml

41
Q

substrate of beta lactamase test

A

nitrocefin

42
Q

encoded by the gene mec A

A

penicillin binding protein 2a

43
Q

automation program for bacterial identification and susceptibility

A

vitek

44
Q

same as vitek but faster

A

maldi tof

45
Q

positive result of chromogenic agar in mrsa detection

A

rose/mauve colored colony

46
Q

detect inducible clindamycin resistance among strains of s. aureus

A

d-test

47
Q

in penicillin resistance, greater than 20mm means

A

sensitive

48
Q

positive result is clover leaf like pattern of zoi

A

modified hodge test

49
Q

used to measure zone of inhibition

A

ruler or caliper

50
Q

carried out to determine susceptibility or resistance of organisms to antimicrobial agents

A

antibiotic susceptibility testing

51
Q

uses tergitol as wetting agent

A

kinyoun’s

52
Q

most critical step in gram staining

A

decolorizer

53
Q

quality control for gaspak jar

A

check every use

54
Q

tincture of iodine

A

iodine + 70% alcohol

55
Q

removes pathogens in food

A

pasteurization

56
Q

121 deg c for 30 mins at 15 psi

A

for contaminated microbial materials

57
Q

examples of this media are CAP BAP HuBA HoBA SBA

A

enriched media

58
Q

in sputum specimen, poor collection has a presence of

A

alveolar macrophage and columnar cells

59
Q

in the stages of bacterial growth, it is a phase where this happens because of unfavorable conditions

A

death phase/phase of decline

60
Q

giving a safety pin appearance and has bipolar bodies

A

y. pestis

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