BacT Exam 2

Question 1

E. coli: oxidase - or +?

lactose - or +?

Answer 1

oxidase -

lactose +

Question 2

Which enterobacteriaeceae are considered coliform bacteria?

Answer 2

E. coli

Klebsiella

Question 3

Cell associated virulence factors of e.coli

Answer 3

endotoxin, capsule, fimbrial and non-fimbrial adhesions

Question 4

Extracellular virulence factors of e. coli

Answer 4

entertoxins, cytotoxins, siderophores (transport Fe)

Question 5

F4

Answer 5

pigs ONLY

neonates –> weaning

Question 6

F5

Answer 6

often with F41
pigs and bovines
only very YOUNG animals
neonatal diarrhea

Question 7

F6

Answer 7

neonates

extrachromosomal

Question 8

F41

Answer 8

rare, but often with F5
neonates
pigs and bovines

Question 9

F18

Answer 9

edema disease

Question 10

Heat labile enterotoxins

Answer 10

"LT"
more antigenic
activate cAMP
increase Cl and bicarb secretion
decrease Na absorption
high molecular weight

Question 11

Heat stable enterotoxins

Answer 11

“Sta”, “Stb”, “EAST”
low molecular weight
little antigenicity

Question 12

Weaning diarrhea ETEC

Answer 12

F4, F18, heat labile and stable factors, hemolytic

Question 13

Factors affecting infection pressure

Answer 13

hygiene

other piglets with ETEC

Question 14

Factors affecting maternal immunity

Answer 14

Sow: metritis, mastitis, first delivery
Piglet: low birth weight, other infection, too many piglets

Question 15

ETEC pathogenesis in pigs

Answer 15

1) oral uptake
2) ascend from lower bowel
3) colonization of SI villi
4) eventually cause septicemia

Question 16

Symptoms of ETEC in pigs

Answer 16

remain drinking
smelly feces, diarrhea
dehydration

Question 17

Is there a vax for pig ETEC?

Answer 17

yes.

Question 18

Virulence factors of VTEC - pigs

Answer 18

Adhesion to SI: F18, 10 days and on
Exotoxin: VT2e or Stx2e –> tunica media necrosis
Endotoxin: acute mortality

Question 19

Some symptoms of VTEC? pigs

Answer 19

multiple piglets affected
hoarse voice
eye lids swollen
anorexic
edemetis
CNS signs
dyspnea

Question 20

Why might you still see symptoms in pigs not yet affected by VTEC post vaccine?

Answer 20

the toxin incubates for 2-3 days, so they could have been infected when you vaccinated them, just not have been showing clinical signs yet

Question 21

Which e. coli of pigs demonstrates diarrhea AT weaning?

Answer 21

EPEC (somewhat rare)

Question 22

Some virulence factors of EPEC in pigs?

Answer 22

eae +

T3SS

Question 23

What is the most important pathogen of pig UTIs?

Answer 23

E. coli

Question 24

How do you treat pig UTI caused by e. coli?

Answer 24

cull!!!

or antibiotics for 3 weeks

Question 25

What does MMA of PPDS stand for?

Answer 25

Metritis Mastitis Agalactiae of Post Partum Dysgalactiae Syndrome

Question 26

Septicemia of pigs with e. coli –> primary or secondary infection?

Answer 26

secondary to enteric infection

Question 27

Who should you vaccinate with bovine ETEC?

Answer 27

mother cows!

Question 28

Virulence factors of ETEC in bovines…

Answer 28

F5, F41, F17
CS31a (related to F4, causes diarrhea in older animals)
Sta, Stb

Question 29

Treatment of bovine ETEC:
if diarrhea and fever?
if no fever?

Answer 29

diarrhea and fever: parenteral therapy

no fever: colistin antibiotic

Question 30

T/F: You should include colostrum from a first calving in a colostrum bank.

Answer 30

False

Question 31

Virulence factors of bovine EHEC

Answer 31

eae +

VT1 and/or VT2

Question 32

Which e. coli of cattle is zoonotic and has a large affinity for peoples’ kidneys?

Answer 32

EHEC

Question 33

Which e. coli are eae + ?

Answer 33

EHEC
EPEC
RPEC