B2: organisation. Flashcards
Give and describe the three types of tissue found in mammals.
- Glandular: contracts in order to move
- Glandular: makes and secretes chemicals.
- Epithelial: covers parts of the body
Order these: organelle, organ system, organ, organism, cell, tissue.
Organelle, cell, tissue, organ, organ system, organism.
What is a tissue?
A tissue is a group of similar cells all working together to carry out a particular function.
What is an organ?
An organ is a group of different tissue that work together to perform a certain function.
What is an organ system?
An organ system is a group of organs working together to perform a certain function.
Define ‘catalyst’
A catalyst is a substance that increases the speed of a reaction without being changed or used up.
Define ‘enzyme’
Enzymes are proteins that act as biological catalysts.
How do enzymes work?
Every enzyme has an ‘active site’ with a unique shape that fits onto the substrate. They reduce the activation energy, allowing the reaction to happen faster.
Why do most enzymes only catalyse one reaction?
For an enzyme to work, the correct substrate has to fit into the enzyme’s unique active site. If the substrate doesn’t match the enzyme’s active site, the reaction won’t be catalysed.
Explain the ‘lock and key’ model of enzyme action.
An enzyme will only work on one substrate. Enzymes and substrates collide to form enzyme-substrate complexes. The substrates are broken down or built up, and the products are released: the enzyme is free to act again.
This theory is known as the ‘lock and key model’.
Explain the ‘induced fit’ model of enzyme action.
The active site is complementary to the substrate, and changes shape a little as the substrate binds to it to get a tighter fit.
What is the optimum temperature for most enzymes?
37°C.
Describe how a change in temperature/pH
Changing the conditions changes the rate of an enzyme-catalysed reaction: At first, increasing the temperature will increase the rate of the reaction, but if it gets too hot, some of the bonds holding the enzyme together break , changing the shape of the active site, so the substrate won’t fit anymore. The enzyme is said to be denatured. The same thing happens if the pH is too high or low, too.
What is the optimum pH for most enzymes?
7.
What is the role of carbohydrases?
Carbohydrases break down carbohydrates into simple sugars.
What is the role of proteases?
Proteases break down proteins into amino acids.
What is the role of lipases?
Lipases convert lipids (fats) into glycerol and fatty acids.
Where is amylase made?
- Salivary glands
- Pancreas
- Small intestine
Where is protease made?
- Stomach
- Pancreas
- Small intestine
Where is lipase made?
- Pancreas
- Small intestine
Where is bile produced?
Bile is produced in the liver.
Where is bile stored?
Bile is stored in the gallbladder.
What is the role of bile?
Bile neutralises stomach acid and emulsifies fats.
Give a method for how a student could investigate the effect of pH on enzyme activity.
- Place a drop of iodine into every well of a spotting tile.
- Place a Bunsen burner on a heat-proof mat, with a tripod and gauze. Put a beaker of water with a thermometer in on top of the tripod, and heat the water until it is 35°C.
- Use a syringe to add 1cm³ of amylase solution and 1cm³ of buffer solution with a pH of 5 into a boiling tube. Put the tube in the heated beaker of water and wait five minutes, keeping the temperature of the water constant.
- With a different syringe, add 5cm³ of starch solution to the boiling tube, mix the contents, and start a stopwatch.
- Use a pipette to take a fresh sample from the boiling tube every 30 seconds, and put a drop into a well. When the iodine remains browny-orange, the starch is no longer present.
- Repeat the whole experiment with buffer solutions of different pHs, and control any control variables to make sure it is a fair test.
What is the role of the mouth in the digestive system? (2)
- Produce amylase enzyme in the saliva
- Mechanically digests & breaks down food through chewing.
What is the role of the stomach in the digestive system? (3)
- Pummels the food with muscular walls
- Produces pepsin (protease)
- Produces hydrochloric acid
What is the role of the liver in the digestive system? (1)
Where bile is produced
What is the role of the gallbladder in the digestive system? (1)
Where bile is stored
What is the role of the pancreas in the digestive system? (1)
- Where protease, amylase and lipase are produced.
What is the role of the large intestine in the digestive system? (2)
- Where excess water is absorbed from food.