B2 - Organisation Flashcards
What 2 words describes an enzyme?
Biological Catalysts.
Function of an enzyme?
Reduce the need of pH or high temperatures for a reaction
What are enzymes made up of?
Proteins -> Amino Acids + Unique Shapes
Where is Des Moines?
Iowa, USA.
What site does every enzyme have and how are they unique?
Every enzyme has an ACTIVE SITE, where only a unique SUBSTRATE can fit.
What is the Lock and Key theory?
Substrate latches onto Enzymes Active site, then the enzyme catalyses the reaction, breaking down the substrate into products.
What are the optimum conditions for Catalysation?
37C,
pH - 7 usually.
What happens if an Enzyme gets too hot or cold?
Hot - Denatures, makes bonds in enzyme break.
Cold - Rate of reaction within enzyme slows.
What enzyme has a pH of 2 to break down protein in the stomach?
Pepsin.
What enzyme catalyses the breakdown of starch to maltose?
Amylase.
What is the IV in the practical “Investigating Enzyme Reactions”?
pH and how it affects amylase.
Describe needed for the practical ‘Investigating Enzyme Reactions’.
Spotting Tiles
Bunsen Burner
Gauze
Tripod
Heatproof Mat
Thermometer
Syringe
Amylase Solution
Stopwatch
GIVE ALL STEPS FOR THE PRACTICAL ‘INVESTIGATING ENZYME REACTIONS’
- Place a drop of iodine solution in every spotting tile
- Place a Bunsen burner on a heatproof mat, and a tripod and gauze over it. Put a beaker of water on top until it’s 35C, and use a thermometer.
- Using a syringe, add 1cm³ of amylase solution, and 1cm³ of buffer solution, ( WITH A PH OF 7) to the boiling tube. Put the test tube into the beaker and wait for 5 mins.
- Use different syringe to add 5cm³ of starch solution.
- Mix the contents of the boiling cube and start a stopwatch,
- Use continuous sampling, taking a drop of the mixture every 30 seconds into spotting tiles.
- Repeat and control variables, with different pH values.
What’s the equation for the Rate of Reaction and its unit?
RATE = 1000 / TIME
Units = S (^-1)
When was the Easter Rising?
April 16-27 1916, Dublin, Ireland.
Why do enzymes even break substrates down?
As most of them are too big to pass through the digestive system and into the blood.
Where is Amylase produced?
Salivary Glands
Pancreas
Small Intestine
What enzyme catalyses the breakdown of protein to amino acids?
Protease
Where is Protease produced?
Stomach {PEPSIN}
Pancreas
Small Intestine