B2 Experiments

Question 1

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Answer 1

4) Record the colour change. If it turns blue-black then starch is present. When the iodine solution no longer turns blue-black, the starch has been broken down by the amylase.

Question 2

Method for measuring the rate of an enzyme controlled reaction?

Answer 2

1) You can measure the rate of reaction by using amylase as the enzyme and starch as the substrate.
2) Amylase catalyses the breakdown of starch, so you can time how long it takes for the starch to disappear.
3) Regularly take a drop of amylase and starch mixture. Add it to a drop of iodine solution on a spotting tile.