B1.1 The World of the Microscope Flashcards
-How microscopy techniques have developed over time -The differences in magnification and resolution between a light microscope and an electron microscope -How to calculate the magnification, real size, and image size of a specimen
What are the symbols for each unit of distance?
Kilometre = km Metre = m Centimetre = cm Millimetre = mm Micrometre = μm Nanometre = nm
1km = ____m
1000m
1m = ____cm
100cm
1cm = ____mm
10mm
1mm = ____μm
1000μm
1μm = ____nm
1000nm
When were light microscopes invented?
During the mid-17th century
What do light microscopes do?
They use a beam of light to form an image of an object (The best magnify up to around x2,000)
when was the electron microscope invented?
During the 1930s
What do electron microscopes do?
They use a beam of electrons to form an image and can magnify objects up to x2,000,000
Why are electron microscopes better than light microscopes?
Electron microscopes have a higher resolution and magnification than that of a light microscope
What is the equation (including the different variations) including magnification, image size and actual size?
I = MA M = I/A A = I/M
What is the resolving power of a light microscope?
200nm
What is the resolving power of a scanning electron microscope?
10nm
What is the resolving power of a transmission electron microscope?
0.2nm