B1 - Cell Biology Flashcards
Do prokaryotic cells have membrane bound organelles?
. Have no nucleus
. No membrane bound organelles
. Contain ribosomes only
What do eukaryotic cells contain?
. Have a nucleus which contains DNA
. Contain membrane bound organelles
Which cells are larger - eukaryotic or prokaryotic?
Eukaryotic
Cells are measured using the unit of …
Micrometers (ųm)
The human eye can only see … ųm sized objects?
50
1 micrometer (ųm) = …..
1 thousandth of 1 millimetre
Put in order (smallest to largest) - chromosome, nucleus, gene, cell, and DNA.
. Gene
. Chromosome
. DNA
. Nucleus
. Cell
In bacteria cells, what is the function of the pilus?
. Pilus - small hairs around the bacteria cell
. Help bacteria cells stick to one another or to surfaces
In bacteria cells, what is the function of the slime capsule?
Protects bacterium from white blood cells and helps groups of bacteria stick together.
In bacteria cells, what is the function of the flagellum?
. Flagella - tail on the bacteria cell
. Aids movement of the bacteria through fluids by rotation
What are plasmids?
Small loops of extra dna
Are yeast cells eukaryotic or prokaryotic?
Eukaryotic
What is the function of mitochondria?
Provide energy - site of respiration
What is the function of ribosomes?
Protein synthesis
What is the function of cytoplasm?
Site of chemical reactions - contains enzymes
What is the function of the cell membrane?
Control what enters and exits the cell
What are chromosomes?
Coiled up lengths of DNA molecules.
What is the function of a nucleus?
Contains generic material that controls the activities of the cell
What is the function of a cell wall?
Supports and strengthens plant cell - made of cellulose
What is the function of a permanent vacuole?
Contains cell sap (a weak solution of sugar and salts)
What is the function of chloroplasts?
Site of photosynthesis
Contains chlorophyll to absorbs light
What is magnification?
How much an image has been enlarged compared to the actual size of the object
What is the equation for magnification?
Magnification = image size ÷ real size
What is resolution?
The ability to distinguish between two objects that are very close together
What does a light microscope do?
Why do you need iodine solution to use a light microscope?
It uses light to make cells visible.
Iodine solution is a stain used to highlight objects in a cell by adding colour to them.
What does an electron microscope do?
Fires electrons at dead samples to form an image.
What are the differences between a light microscope and an electron microscope?
Light microscope:
Lower resolution
Lower magnification
Cheaper
Electron microscope:
Higher resolution
Higher magnification
Expensive
How do you prepare a slide (a strip of clear glass or plastic) when using a light microscope?
- Add a drop of water
- Cut up an onion and separate it out into layers. Use tweezers to peel off some epidermal tissue from the bottom of one of the layers.
- Using the tweezers, place the epidermal tissue into the water on the slide.
- Add a drop of iodine solution
- Get a cover slip (a square of thin, transparent plastic or glass) and stand it upright in the slide, next to the water droplet. Then carefully tilt and lower it so it covers the specimen.
Try not to get any air bubbles because they will obstruct your view of the specimen.
How do you use a light microscope to look at the slide you’ve prepared?
- Clip the slide onto the stage
- Select the lowest-powered objective lens (the one that produces the lowest magnification)
- Use the coarse adjustment knob to move the stage up, just below the objective lens
- Look down the eyepiece and use the coarse adjustment knob to move the stage downwards until the image is roughly in focus.
- Adjust the focus with the fine adjustment knob, until you get a clear image.
- If you need to see the slide with a greater magnification, swap to a higher-powered objective lens and refocus.
What is the function of muscle cells?
Contract and relax to move the body