B1 Flashcards
Eukaryotic Cell
A cell with genetic material in a nucleus
Ranges between 10 and 100 nanometers
E.g Animal cells, plant cells
Prokaryotic Cell
Cells with genetic material floating about in the cytoplasm (no nucleus)
Ranges between 1 and 10 nanometers
E.g bacterium
Animal Cell Structure
Nucleus - Controls the activities of the cell, contains genetic material, and contains instructions to make new cells/organisms
Mitochondria - Where respiration occurs
Cell membrane - A selective barrier that controls which substances pass in and out of the cell
Cytoplasm - A jellylike substance which hosts the chemical reactions that keeps the cell alive, and hosts the other sub cellular structures
Plant Cell Structure
Nucleus - Controls the activities of the cell, contains genetic material, and contains instructions to make new cells/organisms
Mitochondria - Where respiration occurs
Cell membrane - A selective barrier that controls which substances pass in and out of the cell
Cytoplasm - A jellylike substance which hosts the chemical reactions that keeps the cell alive, and hosts the other sub cellular structures
AND
Chloroplast - Contain chlorophyll, that transfers energy from the Sun to the plant via light. Chlorophyll causes the chloroplast to be green, causing parts of the plant with chloroplasts to be green
Vacuole - A sac full of cell sap (solution of sugar and salts). It helps keep the cell rigid, and therefore supports the entire plant and keeps it upright
Cell Wall - A tough substance called cellulose that surrounds the cell. It makes the wall rigid and supports the cell
Structure of (most) Prokaryotes
Cell Wall
Cell Membrane
Cytoplasm
Some bacterial (prokaryote) cells also have
Flagellum - A tail-like structure that allows the cell to travel through liquids
Pili - Tiny hairlike structures that enable the cell to attach to other structures, e.g cells in your digestive tract. In bacteria they’re also used to transfer genetic material
Slime capsule - a thin layer outside the cell wall that protects bacteria from drying out, from toxic substances, and allows it to stick to smooth surfaces
Plasmid - a circular piece of DNA that stores extra genes that may be needed in times of stress, e.g. genes for antibiotic resistance
Light microscope
A microscope that uses light to observe small structures in detail
Light microscope method
- Move the stage to its lowest position
- Select the objective lens with the lowest magnification
- Place the slide, with cells on it, on the stage
- Turn the coarse knob slowly until you see a (usually blurred) image of the object
- Turn the fine focus knob slowly until you see a clear image of the object
- To see cells in greater detail, repeat the steps above, but use a different objective lens
Magnification equation
Total magnification = eyepiece lens magnification x objective lens magnification
Stains
Used to make colourless cells easier to observe, or highlight specific sub cellular structure
Common stains:
Methylene blue - nucleus of an animal cell
Iodine solution - nucleus of a plant cell
Crystal violet - cell walls of bacteria
Electron microscope
Uses electrons instead of light to produce an image
Transmission Electron Microscope
Produce the most magnified images
Work like light microscopes, but instead a beam of electrons passes through a very thin slice of an object
Scanning Electron Microscope
These produce a 3D image of a surface by sending a beam of electrons across the surface
Light Microscopes vs Electron Microscopes
Light Microscope: Cheap to buy and easy to operate Small and portable Simple to prepare a sample Specimens can be living or dead (light microscopes need a dead specimen)
Electron microscope:
Gives black and white images, but colour can be added (light microscopes give the natural colour unless a stain is used)
Resolution: 1x10^(-10) vs light microscope - 2x10^(-7)
DNA
Deoxyribonucleic acid. This substance contains all the instructions that determine your characteristics
Chromosome
A long strand of DNA. Half of all your chromosome are inherited from your mother, the other half from your father
Gene
A short section of DNA. The code of a gene contains specific proteins to be made. These proteins determine the cell’s function
DNA is…
Made up of two strands, forming a double helix
Made up of small units called nucleotides
A polymer
Nucleotide Structure
Circle - phosphate
Pentagon - sugar (deoxyribose)
Rectangle - base
There are 4 different types of nucleotide, because there are 4 different bases. These are:
A - adenine
T - thymine
C - cytosine
G - guanine
Complementary Base Pairing
When bases bond together to hold the two strains of DNA together. A base from one strand bonds with the base from another.
A-T, C-G