AVCL 2 Flashcards
Originates from the fat that many, but not all, members of this class of have the general molecular formula Cn(H2O)n and thus were once considered hydrates of carbon.
Carbohydrates
The simplest carbohydrates which can be aldoses or ketoses.
Monosaccharides
How many carbons does Monosaccharides have?
3-6 carbons
Ribose, Glucose, and Fructose are an example of what type of carbohydrates?
Monosaccharides
Two monosaccharides may be combined together with the loss of one molecule of water to form what?
Disaccharides
What is the bond that connects 2 monosaccharide units in a disaccharide?
Glycosidic Bond
Sucrose (table sugar) and Lactose (Milk sugar) are an example of what type of carbohydrates?
Disaccharides
It is consist of many monosaccharides linked together.
Polysaccharides
Starch, Pectin, Glycogen, and Cellulose are examples of what type of carbohydrates?
Polysaccharides
What are the 6 unknown carbohydrates mentioned in AVCL 2.1?
- Glucose
- Fructose
- Lactose
- Sucrose
- Starch
- Water
How many ml of each unknown carbohydrates must be poured into the test tubes for the tests?
2 ml
This test confirms the presence of carbohydrates in the analyte.
Molisch’s Test
How many ml of Molisch’s Reagent must be poured during the Molisch’s Test?
1 ml of Reagent
In what angle must be poured during the Molisch’s Test?
45 degrees
What must be the color of the positive result of the solution during the Molisch’s Test?
Purplish-Red Rings
A test used to determine of reducing sugar
Benedict’s Test
How many ml of Benedict’s Reagent must be poured during the Benedict’s Test?
3 ml
What is the heating method used in Benedict’s Test and how many minutes will it take to finish?
Boiling water bath in 2 minutes
What must be the color of the positive result of the solution during the Benedict’s Test?
Yellow to Brick Red Precipitate
A test used in differentiating monosaccharide from disaccharide.
Barfoed’s Test
How many ml of Barfoeds Reagent must be
How many ml of Barfoed’s Reagent must be poured during the Barfoed’s Test?
3 ml of Barfoed’s Reagent
What heating method must be used in Barfoed’s test and how many minutes will it take?
Boiling water bath for 5 minutes.
What must be the color of the positive result of the solution during the Barfoed’s Test?
Brick Red Precipitate
A test used to determine aldoses from ketosis
Seliwanoff’s Test
How many ml of Seliwanoff’s reagent must be poured during the Seliwanoff’s test?
3 ml of Seliwanoff’s reagent
What heating method is used in Seliwanoff’s test and how many minutes will it take?
Boiling water bath in 2 minutes
What must be the color of the positive result of the solution during the Seliwanoff’s Test?
Cherry Red Solution
TRUE or FALSE:
Fructose by nature is an aldose.
FALSE
Fructose by nature is a KETOSE
TRUE or FALSE
Succrose is a disaccharide
TRUE
A test used to identify pentoses from hexoses.
Bial’s Test
How many ml of Bial’s Reagent must be poured during the Bial’s Test?
2 ml of Bial’s Reagent
What is the heating method used in Bial’s Test?
BUNSEN BURNER FLAME until liquid begins to boil
What must be the color of the positive result of the solution during the Bial’s Test?
Bluish-Green Color
A test used to identify polysaccharide in the laboratory
Iodine Test
How many drops of iodine is needed during the Iodine Test?
1 drop
What must be the color of the positive result of the solution during the Iodine Test?
Blue-Black Colorization