Autopsy (Postmortem Examination) Flashcards

1
Q

-Gold standard for confirmation of a medical disease
-Wherever scientific medicine of high quality is practiced, postmortem exams are performed
-Whenever a conscientious physician knows why he lost his patient, a postmortem exam has been performed
-Whenever criminal law is enforced
-Whenever a death certificate shows accurately the causes of death & confirmed medical diagnosis for the assembling of vital statistics, a postmortem has been performed
-Whenever there is medical research on the causes & nature of diseases such as cancer, heart diseases & stroke, the investigative method is the postmortem exam
-An informed society requires a postmortem exam in human death for the good of medical science, for the public’s health & for the future care of the living patient

A

Autopsy

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2
Q

Types of autopsy

A
  1. Complete autopsy
  2. Partial autopsy
  3. Selective autopsy
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3
Q

Type of autopsy

  • Requires consent
  • Complete examination of all organs, including the brain
A

Complete autopsy

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4
Q

Type of autopsy

  • Part of the anatomy
A

Partial autopsy

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5
Q

Type of autopsy

  • Restricted to at least a single organ (Ex. MI – heart)
A

Selective autopsy

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6
Q
  1. Written consent from the next kin-abide by the extent or restrictions allowed
    - Relative: oriented by the attending physician, not the pathologist
  2. Death certificate (Old: Blue form | New: Blue border/frame)
    - Signed by:
    a. Physician
    b. Pathologist (back): will sign when PME has been performed
  3. Medical abstract or clinical data
  4. Medico-legal clearance
    - Suspicious evidence of foul play
    - Ex. physical injury
A

Preliminaries for PME

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7
Q

Other Uses of Death Certificate

A

Burial & cremation purposes
Transport of body from hospital funeral cemetery
Medical insurance claiming
- If suicide: (-) insurance
- Acts of God (lightning, flood): (-) insurance
- Civil war: (-) insurance

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8
Q

PME is permitted w/o consent in the following circumstances

A
  1. When it is ordered by the police or coroner (NBI)
  2. When it is necessary to complete the death certificate
  3. When the deceased himself has given consent before he died (advanced directive)
    - Stipulate that in the event you will die, you will be giving out a consent for autopsy
    - Donate your organs for medical purposes or for transplantations
  4. Deceased military personnel who dies in active services/training duty or military services
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9
Q

If pathologist is not available

A

The medico-legal examiner or the coroner has jurisdiction in medico-legal cases & may authorize the pathologist to proceed w/ an autopsy

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10
Q

The coroner has authority in the following cases: (Give atleast 3)

A
  1. All natural deaths occurring in the hospital w/in 24 hrs of admission, unless the case was attended by a private physician w/in 36 hrs of death
  2. Newborns in the 1st 24 hrs of life
  3. All injury cases, old or recent
  4. All deaths due to unknown cases
  5. All deaths due to suspicious cases
  6. All abortion, whether self-induced or otherwise
  7. All violent deaths
  8. All accidental deaths
  9. All sudden deaths
  10. All cases w/o medical attendance w/in 36 hrs prior to the hour of death
  11. All deaths due to drowning, hanging or strangulation (asphyxia)
  12. All deaths due to shooting, stab wounds, burns, electricity, lightning, tetanus, etc.
  13. Homicides
  14. All suicides
  15. All poisoning
  16. Stillborn = omission
  17. Premature death
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11
Q

Death of an organism
Cessation of circulation & respiration (1960’s)

A

Somatic death

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12
Q

T/F:

Clinically dead & dead are the same.

A

T

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13
Q

cessation of heartbeat & respiration but the brain is still alive but injured

A

Clinical death

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14
Q

Criteria for the pronouncement of death

A
  1. Advanced resuscitation techniques that are capable of reviving effectively cases of clinical death
  2. Advance life-sustaining equipment capable of maintaining cardiovascular & respiratory functions despite severe brain injury
  3. Redefinition from cessation to irreversible cessation of cardiorespiratory functions after resuscitation attempts
  4. Brain death: cannot be revived anymore [National institute of neurological diseases & stroke in the US (1977)]
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15
Q

perpetual state of deep sleep

A

Brain death

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16
Q

Criteria for brain death

A

a. Coma (patient will not respond) & cerebral unresponsiveness
b. Apnea
c. Absent cephalic (brainstem) reflexes
d. Electrocerebral silence
criteria should be present for 30 mins at least 6 hrs after onset of coma & apnea

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17
Q

American Bar association & National Conference of Commission of Uniform State Laws Legislative Definition of Death (1980)

A
  1. irreversible cessation of circulation & respiratory functions
  2. Irreversible cessation of all functions of the entire brain, including the brainstem is dead
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18
Q

Death:
1. Coma
2. Absence of the following:
- Motor response
- Pupillary response to light & pupils at mid-position
- Corneal reflexes
- Caloric responses
- Gag reflexes
- Coughing in response to tracheal suctioning
- Sucking & rooting reflexes

A

Death according to American academy of Neurology

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19
Q
  • 1st demonstrable change after death is cooling of the body
  • At room temp: 2’-2.5’F/hr (1st hr)
  • 1.5-2’F/hr (next 12 hrs)
  • 1’F/hr (next 12-18 hrs)
  • As a rule, the body cools at 1.5’F/hr (50% of cases)
  • Not a reliable indicator as to the time of death
A

Algor mortis

20
Q
  • Rigidity of the body due to hardening of the skeletal muscles caused by a series of physiochemical events after death
  • (–chemical bodies between actin & myosin
  • This interlocking is fixed & produces rigor mortis w/o shortening of the muscles
  • Sets w/in 2 hrs after death (head & neck)
  • Complete w/ 12 hrs
  • Persists about 3-4 days
A

Rigor mortis

21
Q
  • Blood supply gravitates to the skin vessels w/c becomes toneless & dilate after circulation ceases
  • Becomes evident as early as 20 mins after death
  • Fully evident w/in 4-8 hrs
  • Tardien spots: petechiae
A

Livor mortis (postmortem lividity/hypostasis)

22
Q
  • Abdomen: green
  • Formation of sulfur gases (bacteria)
A

Discoloration of tissue

23
Q

Enumerate the postmortem changes.

A
  1. Algor mortis
  2. Rigor mortis
  3. Livor mortis (postmortem lividity/hypostasis)
  4. Postmortem clotting of blood
  5. Discoloration of tissue
  6. Putrefaction
  7. Dessication (mummification)
24
Q

Organs removed & dissected individually in the body
Most widely used metohd

A

Technique of Rokitansky

25
Q

En bloc technique

A

Technique of Ghon

26
Q

En masse technique
♫ En masse:
- All organs of thoracic, abdominal, & pelvic are removed at the same time
- Sweeping of all organs

A

Technique of Letulle

27
Q

Very popular, easy to do, convenient
Part of consent: organs should be retained completely or partially
Organs set aside later
Body undertaker of the body

A

Autopsy: Larynx Rectum

28
Q

Tissue specimen Watchglass (isotonic solution)
BF/PC microscope

A

Teasing/Dissociation

29
Q

Tissue (<1mm)
Sandwich bet. 2 slides/coverslip
Vital stain

A

Crushing/squash preparation

30
Q

Spread lightly over a slide (wireloop/applicator)

A

Smear preparation

31
Q

Fresh Tissue Examination preparation (3)

A

Teasing/Dissociation
Crushing/squash preparation
Smear preparation

32
Q

(-) Fixative

A

Frozen section

33
Q

Best frozen section

A

Freezing of unfixed tissue

34
Q

To localize hydrolytic enzymes & other antigens

A

Freezing of fixed tissue

35
Q

Derivative of formaldehyde
Fix at 4’C for 18 hrs

A

Formal (formol) calcium

36
Q

most rapid
Isopentane cooled by liquid nitrogen

A

Liquid nitrogen

37
Q

Commonly used methods of freezing

A

Liquid nitrogen
CO2 gas
Aerosol sprays

38
Q

Staining methods
(frozen sections)

A

1) “PATH”
2) Polychrome methylene blue
3) Alcoholic pinacyanol
4) Thionine
5) H & E = progressive, no decolorizer

39
Q
  • w/o decolorizer
  • for frozen sections
A

Progressive H&E

40
Q
  • w/ decolorizer (acid-alcohol)
  • for routine histology staining
A

Regressive H&E

41
Q

1) w/o use of any chemical fixative
2) __________: rapid freezing (-160’C)
3) __________: removal of H2O in the form of ice (-40’C) – vacuum

A

1) Freeze drying:

w/o use of any chemical fixative
2) Quenching: rapid freezing (-160’C)
3) Sublimation: removal of H2O in the form of ice (-40’C) – vacuum

42
Q

Similar to freeze drying but:
Frozen tissue Rossman’s formula/1% acetone
Dehydrated in absolute alcohol

A

Freeze-substitution

43
Q

Any microtome
Uses CO2
Knife: -40 to -60’C
Tissue: 5 to -10’C
Environment: 0 to -10’C

A

Cold knife procedure

44
Q

Temperature: -18 to -20’C
_________: refrigerated cabinet w/ rotary microtome

A

Cryostat procedure
(Cold microtome);

blank= cryostat

44
Q

Temperature: -18 to -20’C
_________: refrigerated cabinet w/ rotary microtome

A

Cryostat procedure
(Cold microtome);

blank= cryostat

45
Q

Best mounting media for cryostat sections

A

O.C.T. (Optimal Cutting Temperature)