Autoimmune Serology

Question 1

non extractable nuclear antigens

Answer 1

ds-DNA
Histone
Nuclear RNA

Question 2

Extractable nuclear antigens

Answer 2

SM
RNP
Ro (SSA)
La (SSB)
Scl-70 (topoisomerase I)
Jo-1

Question 3

What does the ANA titer represent

Answer 3

the last doubling dilution in order to produce a sample with no fluorescence

Question 4

homogenous/diffuse nuclear fluorescence ANA pattern

Answer 4

anfi-dsDNA (SLE)

Question 5

peripheral (rim) nuclear fluorescence ANA pattern

Answer 5

anti-dsDNA (SLE)

Question 6

speckled nuclear fluorescence ANA pattern

Answer 6

anti-U1 RNP
anti-Ro and La
= MCTD, Sjogren

Question 7

centromeric nuclear fluorescence ANA pattern

Answer 7

kinetochore (CREST)

Question 8

nucleolar nuclear fluorescence ANA pattern

Answer 8

antifibrillarin (SSc)

Question 9

what % of pop has + ANA, how does age affect chance of + ANA?

Answer 9

5%, increases + with age (15% of pts > 55 yo have elevated ANA titer with no clinical significance)

Question 10

Ab a/w lupus nephritis

Answer 10

anti-dsDNA, anti-Sm, anti-C1q

Question 11

Ab a/w neuropsychiatric LE

Answer 11

anti-rRNP

Question 12

Ab a/w photosensitvity

Answer 12

anti-Ro and La

Question 13

Ab a/w neonatal lupus/congenital heart block

Answer 13

anti-Ro and La

Question 14

Ab a/w drug induced LE

Answer 14

anti-histone

Question 15

Ab a/w SLE with polymyositis

Answer 15

anti-Ku (p70/80 nucleolar protein)

Question 16

Ab a/w possible risk of SLE in DLE pts

Answer 16

anti-ssDNA (denatured DNA)

Question 17

Ab a/w SCLE, mild SLE with limited systemic involvment

Answer 17

anti-U1RNP

Question 18

Abs a/w Sjogren

Answer 18

anti-alpha-fodrin, Ro, La

Question 19

Ab in Sjogren a/w inc risk of lymphoma and systemic dz

Answer 19

anti-Ro

Question 20

Ab a/w rapid onset of SS with severe disease and diffuse cutaneous involvement

Answer 20

anti-RNA polymerase

Question 21

Ab a/w pulm HTN in SS

Answer 21

anti-fibrullarian (U3RNP)

Question 22

Ab a/w CREST syndrome

Answer 22

anti-centromere

Question 23

Ab a/w p70/p80 nucleolar protein

Answer 23

anti-Ku (SS)

Question 24

Ab a/w diffuse skin disease in SS and interstitial lung dz

Answer 24

anti-scl-70

Question 25

Ab a/w amyopathic DM

Answer 25

anti-155 kDa and anti-Se

Question 26

Ab a/w cancer associated DM

Answer 26

anti-155 kDa and anti-Se

Question 27

Ab a/w antisynthetase syndrome

Answer 27

anti-Jo1, PL-7, PL-12

* i.e. raynauds, mechanics hands, pulm fibrosis, arthritis, myositis

Question 28

Ab a/w cardiac involvement in DM, severe DM/PM, poor prognosis

Answer 28

anti-SRP

Question 29

Ab a/w good prognosis of DM

Answer 29

anti-Mi2

Question 30

Ab a/w PM/PM overlap with scleroderma

Answer 30

anti-PM/Scl&raquo_space; anti-Ku

Question 31

Ab a/w PM/PM overlap with SLE

Answer 31

anti-Ku

Question 32

Ab a/w MCTD

Answer 32

anti-U1RNP