Autoantibodies & Stains

Question 1

SLE Auto AB’s?

Answer 1

• ANA (nonspecific)
• Anticardiolipin and lupus anticoagulant
• Anti dsDNA, anti- smith

Question 2

Anti- histone

Answer 2

Drug induced lupus

Question 3

Anti U1 RNP (ribonucleoprotein)

Answer 3

mixed CT disease

Question 4

RA?

Answer 4

• Rheumatoid factor, an IgM Ab against IgG Fc region
• Anti-CCP (more specific)

Question 5

Anti-RO/SSA and Anti La/SSB?

Answer 5

Sjogren syndrome

Question 6

Anti Scl70 (anti DNA topiosomerase I)

Answer 6

Scleroderma (diffuse)

Question 7

Anticentromere

Answer 7

Limited scleroderma (CREST Syndrome)

Question 8

Antisynthetase (anti Jo-1), Anti SRP, and Antihelicase (anti-Mi-2)?

Answer 8

Polymyositis and dermatomyositis

Question 9

Autoimmune Hepatitis type 1

Answer 9

Anti smooth muscle

Question 10

Primary biliary cholangitis?

Answer 10

Antimitochondrial

Question 11

MPO-ANCA/ P-ANCA?

Answer 11

• microscopic polyangiitis
• Eosinophilic granulomatosis with polyangiitis
• UC

Question 12

GPA (wegner’s syndrome)

Answer 12

PR3-ANCA/c-ANCA

Question 13

Primary membranous nephropathy?

Answer 13

Anti phospholipase A2 receptor

Question 14

Bullous pemphigoid?

Answer 14

Anti-hemiddesmosome

Question 15

Anti desmoglein (desmosome)

Answer 15

Pemphigus vulgaris

Question 16

Anto g;utamic acid decarboxylase, islet cell cytoplasmic antibodies?

Answer 16

Type 1 DM

Question 17

Antiparietal cell and anti Intrinsic factor?

Answer 17

Pernicious anemia

Question 18

Anti GMB?

Answer 18

Good pasture syndrome

Question 19

Chromogranin and synaptophysin target and tumors identified?

Answer 19

• Neuroendocrine cells
• Small CC of lung and carcinoid tumor

Question 20

Cytokeratin stain target and tumors identified?

Answer 20

• epitheial cells
• epithelial tumors such as Squamous cell

Question 21

DesMin target and tumors identified?

Answer 21

• Muscle
• Muscle tumors (rhabdomyoscaroma)

Question 22

GFAP target and tumors identified?

Answer 22

• neuroGlia
• astrocytoma and Glioblastoma

Question 23

S-100 target and tumors identified?

Answer 23

• neural crest cells
• Melanoma, schwannoma, langerhands cell histiocytosis

Question 24

Vimentin target and tumors identified?

Answer 24

• Mesenchymal tissue such as fibroblasts, endothelial cells and macrophages
• Mesenchymal tumors (sarcomas), also endometrial and RCC, and mengioma