AUBF Chapter 5 Flashcards
Ma RMT KOOOOO!!
Defined as test using different reagents/methodologies
Confirmatory testing
Using tablets and chemicals
Non-reagent strip testing
Chemical parameters for which they are used
Specific confirmatory test
Slightly acidic
First morning specimen
pH 5.0-6.0
pH of normal random samples
pH 4.5-8.0
Reagents strip must be….
Package in opaque containers w/ dessicant
Clinical significance of pH
Acid base disorders
The precipitation of inorganic chemicals dissolved in the urine forms a
Urinary crystals and renal calculi
Principles of pH
Double-indicator system
Methy red produces a color?
pH?
Red to yellow
pH 4-6
Bromthymol blue produces a color?
pH?
Yellow to Blue
pH 5-9
Reagents of pH
Methy red and bromthymol blue
Clinical significance of protein
Renal disease
Clinical proteinuria is indicated (mg/dL)
30 mg/dL or greater (300 mg/L)
Is often associated w/ early renal disease
Proteinuria
Normal urine contains
Less than 10 mg/dL or 100 mg per 24 hrs
Produce by the renal tubular epithelial cells
Uromodulin
Another name for uromodulin
Tamm-horsfall protein
Caused by conditions affecting the plasma
Prerenal proteinuria
Associated with infection and inflammation
Acute phase reactants
Associated with true renal disease
Renal proteinuria
A persistent benign proteinuria occurs in young adults
Orthostatic proteinuria
Principle of protein
Protein error of indicators
Confirmatory test in protein
SSA (sulfosalicylic acid precipitation test)
Another name for micral test
Roche diagnostics
Indianapolis, IN
A gold labeled antihuman albumin antibody enzyme (strips)
Micral test reagents strips
Another name for immunoDip
Sakisui diagnostics
Framingham, MA
Uses a immunochromographic technique
ImmunoDip reagent strip
Confirmatory test for glucose
Glucose test
Most frequently peformed chemical analysis on urine
Glucose test
Principle of glucose
Double sequential enzyme reaction
Chromogens: multistix; color
Potassium iodide
Green to brown
Chromogens: chemstrip; color
Tetramethybenzidine
Yellow to green
Reagents for glucose (M)
Glucose oxidase
Peroxidase
Potassium iodide
Reagent for glucose (C)
Glucose oxidase
Peroxidase
Tetramethylbenzidine
Confirmatory test in glucose
Clinitest (cupper reduction test)
Clinical significance of glucose
D. Mellitus
Pancreatitis
Gestational diabetes
3 intermediate products of fat metabolism and their percentage
Acetone (2%)
Acetoacetic acid (20%)
p-hydroxybutyrate (78%)