AUBF Flashcards
Temperature to dissolve crystals in urine after refrigeration
37 deg c
Standard amount of urine
10 ml to 15 ml
Report the urine if the volume is _____
not adequate
Centrifugation of urine is _____ @ _____
5 minutes @ 400 RCF
Volumes that are frequently used in preparation of sediment
0.5 ml and 1.0 ml
recommended volume in conventional glass slide method
20 ul / 0.02 ml
In examining the urinary sediment, crystals in urine is best seen using _____
HPO
detects casts and ascertain the general composition of sediments
LPO
point of reference in microscopic examination
Epithelial cells
What is the recommended scanner to locate the casts near the edges of the cover slip?
Low power scanner
Reporting of microscopic examination: Average # per LPF
Casts
Reporting of microscopic examination: Average # per 10 HPF
RBCs and WBCs
Most frequently used stain in urinalysis
Steinheimer-Malbin Stain
components of steinheimer-malbin stain
Crystal violet and safranin O
4 stains used to confirm the presence of triglyceride, neutral fats and cholesterol
lipid stains
oil red o
sudan III
Polarizing microscope
identification of bacterial cast, which can be confused from granular casts
Gram stain
V-I-A-S means
crystal violet
iodine
alcohol
safranin
gram (+) bacteria stains
purple/violet
Gram (-) bacteria stains
red/pink
preferred stain for urinary eosinophils
Hansel stain
components of hansel stain
methylene blue and eosin y
prussian blue stains
iron
most frequently used in clinical laboratory; object appears dark against a light background
Bright-field microscopy
Aids in identification of Treponema pallidium
Dark-field microscopy