AUBF Flashcards

1
Q

Temperature to dissolve crystals in urine after refrigeration

A

37 deg c

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2
Q

Standard amount of urine

A

10 ml to 15 ml

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3
Q

Report the urine if the volume is _____

A

not adequate

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4
Q

Centrifugation of urine is _____ @ _____

A

5 minutes @ 400 RCF

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5
Q

Volumes that are frequently used in preparation of sediment

A

0.5 ml and 1.0 ml

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6
Q

recommended volume in conventional glass slide method

A

20 ul / 0.02 ml

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7
Q

In examining the urinary sediment, crystals in urine is best seen using _____

A

HPO

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8
Q

detects casts and ascertain the general composition of sediments

A

LPO

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9
Q

point of reference in microscopic examination

A

Epithelial cells

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10
Q

What is the recommended scanner to locate the casts near the edges of the cover slip?

A

Low power scanner

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11
Q

Reporting of microscopic examination: Average # per LPF

A

Casts

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12
Q

Reporting of microscopic examination: Average # per 10 HPF

A

RBCs and WBCs

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13
Q

Most frequently used stain in urinalysis

A

Steinheimer-Malbin Stain

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14
Q

components of steinheimer-malbin stain

A

Crystal violet and safranin O

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15
Q

4 stains used to confirm the presence of triglyceride, neutral fats and cholesterol

A

lipid stains
oil red o
sudan III
Polarizing microscope

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16
Q

identification of bacterial cast, which can be confused from granular casts

A

Gram stain

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17
Q

V-I-A-S means

A

crystal violet
iodine
alcohol
safranin

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18
Q

gram (+) bacteria stains

A

purple/violet

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19
Q

Gram (-) bacteria stains

A

red/pink

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20
Q

preferred stain for urinary eosinophils

A

Hansel stain

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21
Q

components of hansel stain

A

methylene blue and eosin y

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22
Q

prussian blue stains

A

iron

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23
Q

most frequently used in clinical laboratory; object appears dark against a light background

A

Bright-field microscopy

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24
Q

Aids in identification of Treponema pallidium

A

Dark-field microscopy

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25
enhances visualization of elements with low refractive indices such as hyaline casts, mucous threads, mixed cellular cast and trichomonas
phase-contrast microscope
26
aids in identification of cholesterol in oval fat bodies, fatty casts, and crystals
polarizing microscope
27
visualization of naturally fluorescent microorganisms or stained by a fluorescent dye
fluorescent microscopy
28
three-dimensional microscopy image; layer by layer imaging of specimen
interference contrast
29
stain used in cytodiagnostic urine testing
papanicolaou stain
30
used to prepare permanent slides in cytodiagnostic urine testing
cytocentrifugation
31
smooth, non-nucleated biconcave disc
RBC
32
causes glomerular bleeding
dysmorphic rbcs
33
increased in urinary wbc
pyuria
34
exhibit brownian movement
glitter cells
35
largest cell found in urine sediment
squamous epithelial cells
36
largest RTE cell
PCT
37
RTE cells containing non-lipid filled vacuoles
bubble cells
38
lipid containing RTE cells
oval fat bodies
39
RTE cell appear in groups of 3
renal fragment
40
SEC covered with Gardnerella cocobacillus
clue cells
41
spherical-shaped bacteria
cocci
42
rod-shaped bacteria
bacilli
43
gram negative rods frequently associated with UTI
enterobacteriaceae
44
causes DM, vaginal moniliasis, immunocompromised individual in yeast sediments
candida albicans
45
most frequent parasite often associated with vaginal inflammation
Trichomonas vaginalis
46
small refractile oval structures which may or may not contain buds
yeast
47
bladder parasite that can be seem in urine with terminal spine
schistosoma haematobium
48
parasite seen in perianal region; most common fecal contaminant
Enterobius vermicularis
49
thread-like structures in LPO; frequently seen in females
mucus threads/mucus
50
major constituent of mucus
uromodulin
51
only elements found ONLY in urine and is unique to the kidney
Casts
52
Presence of urinary cast is called
cylinduria
53
it has the appearance of true geometrically structure or amorphous material
crystals
54
type of artifacts that is highly refractile sphere with dimpled center
starch
55
artifacts that appear as sphere with a cell wall
pollen grains
56
artifacts that resembles a cast
fiber/hair
57
artifacts that appear as plant and meat fiber or as brown amorphous material
fecal contaminant/feces
58
most frequently seen cast
hyaline cast
59
average number per LPF of hyaline cast (normal)
0-2 / LPF
60
hyaline cast is normally increased in
strenuous exercise heat exposure dehydration emotional stress
61
hyaline cast is pathologically increased in
acute glomerulonephritis pyelonephritis chronic renal disease chronic heart failure
62
greater stasis of urine
rbc casts/blood cast
63
cast that indicates bleeding within the nephron
rbc cast
64
non-bacterial inflammation in WBC cast
acute interstitial nephritis
65
causes bacterial inflammation in wbc cast and bacterial cast
pyelonephritis
66
cast containing RTE cells that indicates advance tubular obstruction
epithelial cell casts
67
contains fatty cats, oval fat bodies, and free fat droplets
lipiduria
68
type of cast frequently associated with nephrotic syndrome, toxic tubular necrosis, DM, and crush injuries
fatty casts
69
cast that result in cellular disintegration
granular cast
70
cast containing multiple cell type
mixed cellular casts