ATP Synthase Flashcards
What are the “motor components” of the ATP synthase and where are they in the mitochondria?
The “c ring” and Y + E components
The C ring is located in F0, Y and E are located in the cytoplasm of the mito
What is the function of the C ring and how does it work?
Protons funnel through the C ring and cause it to “turn” against the “a” component of beta-unit, which has a “b2” arm connecting them
LTO
Loose-Tight-Open the forms of the beta unit
F0 subunit consists of
all the things in the membrane: C ring, a unit, and some of b2
F1 subunit consists of
b2, delta unit, and alpha/beta units
which subunit is the catalytic unit?
the beta
Alpha_subscipt? and beta__subscript?
alpha-3 and beta-3
C+Y+a/C+Y+beta/C+Y+delta/C+alpha+delta/C+gamma+epsilon/C ring+b2+delta/C ring+epsilon+delta/C+beta+epsilon/C+epsilon+gamma
Which is the correct sequence of subunits making the motor component of the ATP synthase?
C+epsilon+gamma
What part of the ATP synthase is considered the “hexamer”?
the alpha-beta component
how are the F1 and F0 components connected?
two ways
interior column: gamma-epsilon stalk
external column: a-b2-delta
what is the role of the proton gradient?
not to form atp but to release it
what do the LTO formations do?
these are beta conformations
in the L form, the beta loosely binds ADP and P
in the T form the ADP and P are ligated
in the O form the new ATP is released
L –> ____ ?
Tight
T –> _____?
O
O–> _____?
Loose
ADP P can be present in what forms?
L and O, but only in O can they “come into” the beta unit
What direction doe the gamma unit turn?
Y, counterclockwise
