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MCB 102 > Assays and Purification > Flashcards

Assays and Purification Flashcards

1
Q

beta galactosidase

A

lactose = galactose + glucose

cuts at beta 1 bond of galactose
have a X-gal bonded to beta 1 of galactose
in presence of beta galactosidase, bond is cleaved
X gal forms dimer give intense blue color

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2
Q

EDTA

A

good chelator of 2+ metal ions

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3
Q

rate zonal sedimentation

A

separate by Svedberg

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4
Q

Isopycnic sedimentation (buoyant density)

A

stop when density is equal

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5
Q

cation exchange

A

CM

carboxymethyl

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6
Q

anion exchange

A

DEAE

diethylaminoethyl

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7
Q

SDS

A

sodium dodecyl sulfate

unfolds and eliminates 3D strucuture
converts polypeptide into stiff rod
coats backbone uniformly
swamps out instrinsic charges of ionizable side chains

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8
Q

detection of released amino acids

A

ninhydrin becaome Ruhemann’s purple

fluorascamine becomes bright blue-green

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9
Q

Fehling’s reaction

A

detects free reducing end

Cu2+ -> Cu+

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MCB 102 (5 decks)

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