Assays Flashcards
1
Q
Golden gate assay
A
Chip with primers attached to it, each for a different SNP.
Has fluorescent nucleotides in the mix, so that when it binds to a certain primer, the “address” can be used to determine which SNP it is
2
Q
Infinium I vs Infinium II assay
A
Infinium I:
beads have SNP attached to it, and labeled fluorescent nucleotides attach one after the the SNP
Infinium II
beads have primers leading up to the SNP, and each nucleotide is labeled differently. The fluorescent SNP is read
3
Q
Compare DNA with CODIS
A
Use PCR on several specific loci,
variable number of STR (length of PCR fragment) used to determine allele.
Differently labeled primers can be used to do multiple loci at once
4
Q
Uses of CODIS system (STR):
5
A
parentage forensic felon database archeological remains disaster identification
5
Q
Types of samples for use in CODIS (8)
A
blood semen tissue bone teeth hair nails saliva
