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PHA6122 LAB Pharmaceutical Analysis 2 > Assay of Acetaminophen Tablets Using HPLC > Flashcards

Assay of Acetaminophen Tablets Using HPLC Flashcards

1
Q

Separation technique in which the components of a sample are distributed between the two phases

A

chromatography

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2
Q

2 phases of chromatography

A
  1. stationary phase
  2. mobile phase
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3
Q

layer or coating on the supporting medium which INTERACTS WITH THE ANALYTES

A

stationary phase

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4
Q

CARRIES THE SOLUTE across the stationary phase

A

mobile phase

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5
Q

Classification according to nature of mobile and stationary phase

A
  1. Normal
  2. Reverse
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6
Q

Polar compounds are highly adsorbed

A

normal

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7
Q

Non-polar compounds are highly adsorbed

A

reverse

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8
Q

Normal
- Stationary Phase: ______________
- Mobile Phase: __________________

A

SP: polar
MP: non-polar

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9
Q

Reverse
- Stationary Phase: ______________
- Mobile Phase: __________________

A

SP: non-polar
MP: polar

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10
Q

Normal chromatography

___________________ (polar/non-polar) phase would be eluted FIRST

A

non-polar

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11
Q

Normal chromatography

___________________ (polar/non-polar) phase would be eluted LAST

A

polar

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12
Q

Reverse chromatography

___________________ (polar/non-polar) phase would be eluted FIRST

A

polar

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13
Q

Reverse chromatography

___________________ (polar/non-polar) phase would be eluted LAST

A

non-polar

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14
Q

Mechanisms of separation

A
  1. adsorption
  2. partition
  3. ion-exchange
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15
Q

Separation involves COMPETING INTERACTIONS between adsorption at the stationary phase and dissolution in the mobile phase

A

adsorption

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16
Q

Particles are SEPARATED ON THE COMPONENTS of the solvent system

A

partition

17
Q

Separation is based mainly on DIFFERENCES IN THE ION EXCHANGE AFFINITIES of the sample components

A

ion-exchange

18
Q

Types of chromatography useful in qualitative and quantitative analyses in USP procedures

A
  1. paper chrom
  2. TLC
  3. column chrom
  4. gas chrom
  5. HPLC
19
Q

HPLC
- Stationary Phase: ______________
- Mobile Phase: __________________

A

SP: solid
MP: liquid solvent system

20
Q

uses same type of solvent system

A

isocratic

21
Q

technique of continuously changing the solvent composition during the chromatographic run

A

gradient elution

22
Q

[T/F] adsorption, partition, or ion-exchange can all be used as the mechanism of separation for HPLC

A

TRUE

23
Q

organic/aqueous solvents are used depending on the analysis

A

mobile phase

24
Q

used to remove dissolved air from the mobile phase

A

degassing unit

25
Q

used to deliver the mobile phase at constant flow

A

solvent delivery pump

26
Q

used to store std soln or sx soln

A

sample vial

27
Q

introduce std soln or real sx to the HPLC column

A

injector

28
Q

used to keep temperature constant

A

column oven

29
Q

used to separate each compound contained in the sample

A

column

30
Q

used to detect the eluted compounds from the column

A

detector

31
Q

apparatus in which the signal from the detector is processed and the chromatogram is displayed

A

workstation

32
Q

The term column includes _________________, _______________________________, and _________________________, packed with a stationary phase

A

stainless steel, lined stainless steel, and polymeric columns

33
Q

The _______________________________ of the column affects the separation, and therefore typical column dimensions are included in the individual monograph

A

length and inner diameter

34
Q

a GRAPHICAL REPRESENTATION of the detector response, concentration of analyte in the effluent, or other quantity used as a measure of effluent concentration versus effluent volume or time

A

chromatogram

35
Q

time elapsed between the injection of the sample and the appearance of the maximum peak response of the eluted sample

A

retention time (tR)

36
Q

a measure of the concentration of the compound it represents

A

area under a peak

37
Q

C8H9NO2

A

acetaminophen

38
Q

acceptance criteria of acetaminophen tablets

A

90.0% - 110.0% on the dried basis

PHA6122 LAB Pharmaceutical Analysis 2 (1 decks)

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