Assay of Acetaminophen Tablets Using HPLC Flashcards
Separation technique in which the components of a sample are distributed between the two phases
chromatography
2 phases of chromatography
- stationary phase
- mobile phase
layer or coating on the supporting medium which INTERACTS WITH THE ANALYTES
stationary phase
CARRIES THE SOLUTE across the stationary phase
mobile phase
Classification according to nature of mobile and stationary phase
- Normal
- Reverse
Polar compounds are highly adsorbed
normal
Non-polar compounds are highly adsorbed
reverse
Normal
- Stationary Phase: ______________
- Mobile Phase: __________________
SP: polar
MP: non-polar
Reverse
- Stationary Phase: ______________
- Mobile Phase: __________________
SP: non-polar
MP: polar
Normal chromatography
___________________ (polar/non-polar) phase would be eluted FIRST
non-polar
Normal chromatography
___________________ (polar/non-polar) phase would be eluted LAST
polar
Reverse chromatography
___________________ (polar/non-polar) phase would be eluted FIRST
polar
Reverse chromatography
___________________ (polar/non-polar) phase would be eluted LAST
non-polar
Mechanisms of separation
- adsorption
- partition
- ion-exchange
Separation involves COMPETING INTERACTIONS between adsorption at the stationary phase and dissolution in the mobile phase
adsorption
Particles are SEPARATED ON THE COMPONENTS of the solvent system
partition
Separation is based mainly on DIFFERENCES IN THE ION EXCHANGE AFFINITIES of the sample components
ion-exchange
Types of chromatography useful in qualitative and quantitative analyses in USP procedures
- paper chrom
- TLC
- column chrom
- gas chrom
- HPLC
HPLC
- Stationary Phase: ______________
- Mobile Phase: __________________
SP: solid
MP: liquid solvent system
uses same type of solvent system
isocratic
technique of continuously changing the solvent composition during the chromatographic run
gradient elution
[T/F] adsorption, partition, or ion-exchange can all be used as the mechanism of separation for HPLC
TRUE
organic/aqueous solvents are used depending on the analysis
mobile phase
used to remove dissolved air from the mobile phase
degassing unit
used to deliver the mobile phase at constant flow
solvent delivery pump
used to store std soln or sx soln
sample vial
introduce std soln or real sx to the HPLC column
injector
used to keep temperature constant
column oven
used to separate each compound contained in the sample
column
used to detect the eluted compounds from the column
detector
apparatus in which the signal from the detector is processed and the chromatogram is displayed
workstation
The term column includes _________________, _______________________________, and _________________________, packed with a stationary phase
stainless steel, lined stainless steel, and polymeric columns
The _______________________________ of the column affects the separation, and therefore typical column dimensions are included in the individual monograph
length and inner diameter
a GRAPHICAL REPRESENTATION of the detector response, concentration of analyte in the effluent, or other quantity used as a measure of effluent concentration versus effluent volume or time
chromatogram
time elapsed between the injection of the sample and the appearance of the maximum peak response of the eluted sample
retention time (tR)
a measure of the concentration of the compound it represents
area under a peak
C8H9NO2
acetaminophen
acceptance criteria of acetaminophen tablets
90.0% - 110.0% on the dried basis
