aseptic techniques Flashcards
designed to provide a barrier between the microorganisms in the environment and the sterile cell culture
aseptic techniques
it is used to inoculate a culture into a broth medium or into an agar deep tube
inoculating needle
used for transferring a small volume of bacteria onto the surface of an agar plate and slant
inoculating loop
this technique is the freeing of an environment from all living microorganisms
sterilization techniques
which physical method uses heat to kill microbes by coagulation of protein, denaturation of enzymes and oxidation
Heat
which sterilization method sterilizes through removing of microbes from fluids by exposing to small size filter.
This is used for heat sensitive fluids like serum, antibiotic, sugar, and urea
Filtration
sterilization method that causes denaturation of proteins and enzymes.
Radiation
what are the sterilization by physical methods
Heat, Filtration, Radiation
what are the sterilization by dry heat methods?
Red hot, flaming, hot air oven, incineration
Hot air oven:
___ *C for ____ mins
___ *C for ____ mins
___ *C for ____ mins
Pre-sterilization at ___ *C for ____ mins
150*C for 15 mins
160 *C for 60 mins
180 *C for 30 mins
Pre-sterilization at 150 *C for 15 mins
Method that allows Exposure of wires and forceps to the burner until it becomes red hot
Red Hot method
Slowly passing of an objects to the flame will reduce the number of microorganisms.
Flaming
Electrical devices which use dry heat to sterilize.
hot air oven
Treating objects to heating over 250 until becomes black.
Incination
Sealed device that kills microorganisms using saturated steam under pressure.
autoclave
