Aseptic Techniques

Question 1

a set of routine measures taken to prevent cultures, sterile media
stocks, and other solutions from being contaminated (Bykowski et
al., 2008)

Answer 1

Aseptic Techniques

Question 2

a series of steps to prevent contamination during manipulations of
cultures and sterile media (Madigan et al., 2015)

Answer 2

Aseptic Techniques

Question 3

Aseptic technique is a series of steps to prevent contamination during manipulations of
cultures and sterile media.

Answer 3

Madigan et al., 2015

Question 4

Aseptic technique is a set of routine measures taken to prevent cultures, sterile media
stocks, and other solutions from being contaminated.

Answer 4

Bykowski et
al., 2008

Question 5

What are the Aseptic Techniques?

Answer 5

1. Wear Appropriate Lab PPE
2. Clean and Disinfect Floor
3. Use UV lamp or laminar flow
   hood
4. Disinfect workbenches
5. Appropriate sterilization
   procedure
6. Handwashing and disinfection
7. Handle microbial cultures with
   caution
8. Effective sterilization of
   inoculating loops
9. Proper dispensing of culture
   media or solutions
10. Limit air exposure of cultures
    and media
11. Avoid breathing on cultures or
    sterile instruments
12. Always work near a flame
13. Disinfect glass slides and
    coverslips before washing
14. Observe proper handling and
    disposal of contaminated materials
15. Decontaminate used glassware, culture
    media, and other materials appropriately

Question 6

Appropriate Lab PPE

Answer 6

1. Long hair tied back
2. Protective eyewear
3. Appropriate hand protection
4. Buttoned up, fitted coat
5. Closed-toe shoes
6. Full-length pants

Question 7

BSL-1, BSL-2, BSL-3, BSL-4

Answer 7

Handle microbial cultures with
caution (BIOSAFETY LEVELs - BSL)

Question 8

Lowest safety level. Not known to cause disease in adult human.

Answer 8

BSL-1

Question 9

Moderate danger if inhaled, swallowed, or exposed to skin.

Answer 9

BSL-2

Question 10

Severe or potentially lethal disease.

Answer 10

BSL-3

Question 11

Highest safety level. Life threatening disease.

Answer 11

BSL-4

Question 12

-No Containment
-Defined organisms
-Unlikely to cause disease
Sample organism: E.coli

Answer 12

BSL-1

Question 13

-Containment
-Moderate risk
Disease of varying severity
Sample organisms: Influenza, HIC, Lyme Disease

Answer 13

BSL-2

Question 14

-High containment
-Aerosol transmission
-Serious/Potentially lethal disease
Sample organism: Tuberculosis

Answer 14

BSL-3

Question 15

-Max containment
-“Exotic”, High-risk Agents
-Life-threatening disease
Sample organism: Ebola Virus

Answer 15

BSL-4