Aseptic Techniques

Question 1

a set of routine measures taken to prevent cultures, sterile mediastocks, and other solutions from being contaminated (Bykowski et al., 2008)

a series of steps to prevent contamination during manipulations of cultures and sterile media (Madigan et al., 2015)

Answer 1

Aseptic Techniques

Question 2

Biosafety Levels

Answer 2

BSL 1- Not known
BSL 2- Moderate
BSL 3- Severe
BSL 4 - Highest

Question 3

Appropriate Lab PPE

Answer 3

Hair tied
Protective Eyewear
Buttoned Up fitted coat
Full length pants
Appropriate hand protection
Closed-toe shoes

Question 4

Use of UV Lamp in Lab

Answer 4

Ultraviolet (UV) light disinfection involves exposing microorganisms to intense UV light. The UV light deteriorates the DNA of the microorganisms, so that they would be unable to reproduce and cause diseases. UV disinfection is effective for bacteria, viruses, and parasites.

-to kill or inactivate microorganisms by destroying nucleic acids and disrupting their DNA, leaving them unable to perform vital cellular functions.

Question 5

Approptiate Sterilization Procedure

Answer 5

Using autoclave machine, it uses high temperature steam and pressure to kill microorganisms such as bacteria, viruses, fungus and spores on items that are placed inside its pressure vessel. Autoclaves are often used as steam sterilizers in healthcare, laboratory, food and pharmaceutical industries.

Question 6

CULTURE MEDIA PREPARATION
General Steps

Answer 6

• Weighing of dehydrated media or media
  ingredients
• Dissolving of components in the solvent
• Checking/adjustment of pH (optional)
• Dispensing into glassware/containers
• Capping or plugging with cotton
• Sterilization (usually at 15 psi for 15 minutes)