ASEPTIC TECHNIQUE Flashcards

1
Q

Microorganisms need to be in the form of ————————–______________ in order for them to be characterized and identified.

A

pure culture

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2
Q
A
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3
Q

Microorganisms grow in ————— medium.

A

sterile nutrient-containing medium

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4
Q

We must have a method of transferring growing organisms (also called ———–) from a pure culture to a sterile medium without introducing any unwanted outside contaminants.

A

inoculum

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5
Q

Techniques necessary when handling organisms to maintain aseptic conditions

A

ASEPTIC TECHNIQUES

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6
Q

Performing lab work that greatly reduces the risk of contamination

A

ASEPTIC TECHNIQUE

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7
Q

Disinfect your working area with the ———–.

A

10%

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8
Q

Necessary lab materials used in aseptic technique

A

bacterial stock culture
growth media
alcohol lamp
transfer tools

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9
Q

proper flame

A

small blue cone

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10
Q

The inoculated needle and loop needs to be heated until they turn ———–

A

orange

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11
Q

allow you to obtain single isolated bacterial colonies originating from a single bacterium or arrangement of bacteria

A

STREAKING PATTERNS

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12
Q

It represents a single type of microorganism that originated from a single cell

A

Each colony

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13
Q

Pond bacteria contains ——- bacteria/mL

A

10^5 to 10^6

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14
Q

Soil contain —— bacteria/mL

A

more than 10^8

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15
Q

E. coli contain ———–bacteria/mL

A

at least 10^7

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16
Q

method employed to plate a liquid sample for the purpose of isolating or counting the bacteria present in that sample

A

SPREAD PLATING

17
Q

method applied for microbial testing of foods or any other samples or to isolate and identify variety of microbial flora

A

SPREAD PLATING

18
Q

USED FOR COUNTING THE NUMBER OF COLONY-FORMING BACTERIA PRESENT IN LIQUID SPECIMEN

A

POUR PLATING

19
Q

Fixed amount of inoculum (1ml) from a broth/sample is placed in the center of sterile petri dish

A

Pour plating

20
Q

molten cool agar

A

approximate 15mL

21
Q

used to determine the number of microbes/mL

A

pour plating

22
Q

method used to obtain pure cultures

A

STREAK PLATING