AS - Unit 1 - Cells Flashcards
What is the maximum magnification of a light microscope?
X1500
What is the definition of magnification?
The degree to which the size of an image is larger than the object itself.
What is the definition of resolution?
The degree to which it is possible to distinguish between two objects that are very close together. The higher the resolution, the greater the detail you can see
What is the highest resolution of the light microscope?
200 nm
What kind of organism can you see under a light microscope?
A living one
What is an artefact?
When a microscope specimen is distorted during the preparation process and therefore isn’t an accurate representation
How do you prepare a specimen for a light microscope?
- Staining - coloured stains are applied to allowed the specimen to be seen.
- Sectioning - specimens are embedded in wax, thin sections are then cut without distorting the structure of the specimen
What is the order of the units of measurement?
Metre (m) Decimetre (dm) Centimetre (cm) Millimetre (nm) Micrometre (um) Nanometre (nm)
What is the highest resolution for the human eye?
100 um
What is the highest resolution for the electron microscope?
0.20nm
How do you calculate magnification and size of a specimen?
Actual size X magnification
What are the two types of electron microscope?
Transmission electron microscope (TEM)
Scanning electron microscope (SEM)
How do TEM’s work??
The electron beam passes through a very thin prepared sample
Electrons pass through the denser parts of the sample less easily giving some contrast in the final image
How does an SEM work??
The electron beam is directed onto a sample
The electrons don’t pass through the specimen they are ‘bounced off’ the sample
Which electron microscope produces a 3D image??
A scanning electron microscope (SEM)
What is the max magnification of a TEM microscope??
X500 000
What is the max magnification of an SEM?
X100 000
Give 3 advantages of the electron microscope?
The resolution is 0.1nm (2000X better than a light microscope)
Can be used to produce detailed images of the organelles inside cells
SEM produces 3D images that can reveal the detail of contours and cellular or tissue arrangements
Give 3 limitations of the electron microscope
Samples have to be dead
Expensive
Preparing samples for and using the electron microscope requires a high degree of skill and training
Give the definition of staining (referring to microscopy)
Refers to any process that helps to reveal or distinguish difference features
What do electron microscope images look like?
Black and white
What is division of labour?
Each organelle has a specific role to play in the function of the cell, all the different organelles work together in a cell, each contributing it’s part to the survival of the cell
What is the cytoskeleton?
A network of fibres made of protein that keep the cell’s shape stable by providing an internal framework
Some fibres are called actin filaments, what role do they play in the cell?
Actin filaments move against each other and move some organelles around inside cells
What are microtubules?
They are cylinders inside the cell that are about 25nm in diameter. They are made of a protein called tubulin. They are used to move a microorganism through a liquid or to waft a liquid past the cell. They move chromosomes during mitosis, vesicles from the er to the Golgi. They use ATP to drive these movements
What is a eukaryote?
A cell with a nuclei
What are flagella or cilia?
Hair-like extensions that stick out from the surface of cells. Each one is made up of a cylinder that contains 9 microtubules arranged in a circle.
What are prokaryotes?
Cells without a nuclei
What are vesicles?
Membrane-bound sacs found in cells. They are used to carry many different substances around the cell
What does the vacuole do in a plant cell??
Maintains cell stability
What is a vacuole filled with and what is its purpose?
Water and solutes, to maintain cell stability and push the cytoplasm against the cell wall making the cell turgid. This helps to support the plant
What are plant cell walls made from??
Cellulose
What is the structure of the nucleus?
Largest organelle
Surrounded by a nuclear envelope
What is the function of the nucleus?
Houses nearly all the cell’s genetic material
What is the structure of endoplasmic reticulum?
Series of flattened, membrane-bound sacs
They are attached to the outer nuclear membrane
Rough ER is studded with ribosomes
Smooth ER does not have ribosomes
What is the function of the endoplasmic reticulum
Rough ER - transports proteins that were made on the attached ribosomes
Smooth ER - involved in making lipids that the cell needs
What is the structure of the Golgi apparatus?
Stack of membrane-bound, flattened sacs
What is the function of the Golgi apparatus?
Receives proteins from the ER and modifies them into vesicles which can then be transported to the surface of the cell so it can be secreted
What is the structure of the mitochondria?
Spherical or sausage-shaped
Two membranes separated by a fluid-filled space
What is the function of the mitochondria?
The site where ATP is produced during respiration
What is the structure of the chloroplasts?
Found only in plant cells and the cells of some protoctists
Two membranes separated by a fluid filled space
What is the function of chloroplasts?
The site of photosynthesis in plant cells
What is the structure of lysosomes?
Spherical sacs surrounded by a single membrane
What is the function of lysosomes?
Break down materials e.g. White blood cell lysosomes help to break down invading microorganisms
What is the structure of the ribosomes?
Two subunits
What is the function of ribosomes?
Site of protein synthesis in the cell, they act as an assembly line where coded information from the nucleus is used to assemble proteins from amino acids
What is the structure of the centrioles??
Small tubes of protein fibres
What is the function of the centrioles?
Take part in cell division, form fibres known as the spindle which move chromosomes during nuclear division
What does the term organelle mean?
Refers to the particular structure of a cell that has a specialised function. Some organelles are membrane-bound, others are not. All perform a particular role in the life processes of the cell
Where are the instructions the make hormones??
In the DNA in the nucleus
Give the 9 steps of protein synthesis
- mRNA copy of the instructions for insulin is made in the nucleus
- mRNA leaves through the nuclear pore
- mRNA attaches to a ribosome on the rough ER, ribosome reads the instructions to assemble the protein
- Insulin molecules are ‘pinched off’ in vesicles and travel towards the Golgi
- Vesicle fuses with Golgi apparatus
- Golgi processes and packages insulin molecules ready for release
- Packaged insulin molecules are ‘pinched off’ in vesicles from Golgi and move towards cell surface membrane
- Vesicle fuses with cell surface membrane
- Cell surface membrane opens to release insulin molecules outside
Give 7 features of prokaryotes
Only have one membrane
Surrounded by a cell wall
Contain ribosomes that are smaller than eukaryotic ribosomes
DNA in the form of a single loop
DNA is not surrounded by a membrane
ATP production takes place in specialised infolded regions of the cell surface membrane called mesosomes
Some prokaryotic cells have flagella
What is the phospholipid bi-layer?
The basic structural component of plasma membranes. It consists of two layers of phospholipid molecules. Proteins are embedded in this layer
Give 5 roles of membranes
Separating cell components from the outside environment
Separating cell components from cytoplasm
Cell recognition and signalling
Holding the components of some metabolic pathways in place
Regulating the transport of materials into or out of cells
Explain the basic structure of a phospholipid
Phosphate head is hydrophilic (water loving)
Two fatty acid tails are hydrophobic (water hating)
How can a bilayer form?
If phospholipid molecules are completely surrounded by water
If phospholipid molecules are mixed with water, how do they arrange themselves?
They form a layer at the water surface, the phosphate heads stick into the water and the fatty acid tails stick up out of the water
How does a bilayer effectively create a membrane?
The hydrophobic layer formed by the phospholipid tails creates a barrier to many molecules and separates the cell contents from the outside world.
Where do most metabolic reactions take place?
In water-based environments
Through which microscopes can you see membranes?
Electron microscopes
How thick are cell membranes?
7-10nm
What is the protein called that allows water molecules through it?
Aquaporin
What is the name for a model of the biological membrane?
The fluid mosaic model
What are the three main features of the fluid mosaic model?
A bilayer of phospholipid molecules
Various protein molecules floating in the bilayer
Some extrinsic proteins partially embedded in the bilayer and some intrinsic proteins spanning the bilayer completely
What is the difference between a glycoprotein and a glycolipid?
Glycolipids are carbohydrates coming straight from the bilayer
Glycoproteins are carbohydrates coming from a protein embedded in the bilayer
What is the role of cholesterol in the fluid mosaic model?
The provide eukaryotic cells mechanical stability. This is by fitting between the fatty acid tails and helps make the barrier more complete, so water and ions cannot pass easily and directly through the membrane
What do channel proteins do in the fluid mosaic model?
They allow the movement of some substances across the membrane. E.g. Glucose
What do carrier proteins do in the fluid mosaic model?
Actively move some substances across the membrane. E.g. Magnesium ions in plant cells
What do receptor sites do in the fluid mosaic model?
Some allow hormones to bind with the cell so that a response can be carried out. They also let drugs bind which affects cell metabolism
What factor causes cell membranes to become ‘leaky’?
Increasing temperature. This gives cells more kinetic energy so they move faster allowing gaps to be made, making it leaky