Application of Reproduction and Genetics

Question 1

pcr

Answer 1

polymerase chain reaction
rapidly amplifies quantity of DNA short tandem repeats
strand separation heat to 95° split into 2
primer binding cool to 55° for primers to bind
strand synthesis heat to 70° for DNA polymerase (Taq) to catalyse complimentary strand synthesis
repeat cycle
gel electrophoresis

Question 2

gel electrophoresis

Answer 2

restriction endonuclease makes fragments
DNA samples loaded into wells, voltage applied
move to positive
smaller fragments move easily