Appilcation of Recombinant techniques

Question 1

Name two feature of PCR that make this technology useful in molecular diagnostics and forensics

Answer 1

Highly sensitive and specificity

Question 2

What are STRs and what makes them useful in forensic science?

Answer 2

The fact that the number of these repeats varies between individuals makes STRs useful in forensic
science when comparing a crime scene DNA sample with that from a suspect.

Question 3

In microarray technology:
a) What must the mRNA be converted to before it may be bound by specific nucleotide probes and how
is this done?

Answer 3

cDNA

Question 4

How would you control the stringency of hybridization?

Answer 4

salt ad temperature concentration

Question 5

Name two limitation of this technology

Answer 5

: This technology is qualitative not quantitative
Very expensive equipment

Question 6

In qPCR, what is the Ct value used to quantify gene expression?

Answer 6

This is the cycle threshold value and is defined as the number of cycles required for the fluorescent signal of a
particular reaction to cross the threshold set by a negative control

Question 7

What is the most common vector used for genetic engineering in plants and where did it originally come from?
What feature makes this plasmid useful in genetic engineering?

Answer 7

The Ti plasmid from Agrobacterium (tumefasciens), a bacterium which causes a disease called Crown Gall in plants. The
plasmid integrates into the plant genome, making it useful in genetic engineering