Anxiolytics Flashcards
Invitro methods for Anxiolytics
1.GABA a receptor binding
2.GABA b receptor binding
3.Benzodiazepine receptor:[³H]-flunitrazepam binding assay
4. Serotonin (5-HT1A) receptor: binding of [3H]-8-hydroxy-2-(di-n-propylamino)-tetralin ([3H]-DPAT)
5. Serotonin (5-HT1B) receptor: binding of [3H] 5 hydroxytryptamine([3H]5-HT)
Invio method of anxiolytics
- Elevated plus maze
- Marble burying test
- Vogle lick test
- Anti-anxiety Dark and light model
- Social interaction in rats
- Staircase test
- Four plate test in mice
- Watermaze test
Parkinsons model invitro
- Dopamine stimulated adenyl cyclase activity
- Radioligand binding studies for D1 and D2 dopamine receptors
- Experiments using rat striatal slices.
- Dopamine release from synaptosomes.
- In vitro neuroprotective efficacy.
Parkinsonism invivo methods
1.Reserpine antagonism.
2.MPTP models in monkeys.
3.Tremorine & oxotremorine antagonism.
4.Circling behaviour in nigrostriatal lesioned rats.
5.Elevated body swing test.
Stepping tests in rats.
Antiarrhytmics invitro method
- Langendorff technique.
- Acetylcholine / Potassium induced arrhythmia.
Anti arrythmics invivo methods
1.Chemically induced arrhythmia
•Aconitine Antagonism in Rats.
•Digoxin induced arrhythmia in Guinea pigs.
•Adrenaline induced arrhythmia.
•Calcium induced arrhythmia.
- Electrically induced arrhythmia
•Ventricular fibrillation electrical threshold.
•Programmed electrical stimulation induced arrhythmia.
Exercise induced ventricular fibrillation.
4.Mechanically induced arrhythmia.
• Reperfusion arrhythmia in rats.
• reperfusion arrhythmia in dogs.
- Genetically induced arrhythmia.
Antihypertensive drugs invivo
- DOCA (Deoxycorticosterone acetate) salt induced hypertension in rats.
- Fructose-induced hypertension in rats.
- Salt sensitive Dahl rats.
Chronic renal hypertension in rats and dogs. - Tail-cuff method.
- Gold blatt hypertension.
- Renin inhibition in monkeys.
- Antihypertensive and vasodilator action in rats.
Antihypertensive invitro
- Monocrotaline induced pulmonary hypertension.
- Beta 2 adreno receptor binding.
ACE inhibition in Guenia pig ileum.
Diabetic invivo models
Chemically induced diabetes
Hormonal induced diabetes
Virus induced diabetes
Surgical methods
Genetic method
Diabetic invitro models
Fructose 2,6 – biphosphate production in rat hepatocytes
Inhibition of alpha amylase
Inhibition of alpha glucosidase
Anti atherosclerotics invivo
1)Triton Wistar rat induced hyperlipidemia
2)Fructose induced hypertriglyceridemia in rats
3)Hypolipidemic activity in Syrian hamsters
4)Cholesterol diet induced atherosclerosis in rabbits
5)Hereditary hypercholesteremia in rats
Hereditary hyperlipidemia in rabbits
Antiatherosclerotic agents invitro methods
- Inhibition of isolated enzyme HMG CoA reductase
- Acetyl CoA cholesterol acyl transferases inhibitory model
Anti cancer invitro
Tetrazolium salt assay
Sulphorhodamine B assay
Dye exclution test
Cell counting assay
3H tymidine uptake aasay
Clonogenic assay
Anticancer invivo
DMBA induced mouse skin papilloma
DMBA induced rat mammary gland carcigenosis
MNV induced tracheal squamous cell carcinoma in hamster
Genetically engineered mouse model
Viral infection model
Transplantation model
Anti asthmatic invivo
Bronchospasmolytic activity in anesthetized guinea pigs
Arachidonic acid or PAF induced respiratory and vascular dysfunction in guinea pigs
Anaphylactic mircoshock is guinea pigs
Seretonin aerosol induced asphyxia in guinea pigs
