Antimicrobial Susceptibility Testing Flashcards
A procedure used to produce antimicrobial susceptibility profile.
Antimicrobial Susceptibility Testing (AST)
A procedure that detects resistance to agents that may be used therapeutically.
Antimicrobial Susceptibility Testing (AST)
Performed after every culture and identification of the specimen.
Antimicrobial Susceptibility Testing (AST)
All are reasons why AST is important, except:
a. Provides data on the susceptibility of bacterial isolates
b. Assists the clinician in selecting a specific antibiotic therapy
c. Does not avoid antibiotic resistance especially if infection is reoccuring.
d. Replaces very expensive, empirically chosen broad spectrum antibiotics.
c. Does not avoid antibiotic resistance especially if infection is reoccuring.
All are standards for AST except:
a. Antibiotic Resistance
b. Thickness of media
c. Nutrients of media
d. Antibiotic
a. Antibiotic Resistance
Action should only be between the antibiotic disc and the bacteria of interest. No environment consideration is required in growing bacteria.
a. Both statements are correct.
b. Statement 1 is incorrect while Statement 2 is correct.
c. Both statements are incorrect.
d. Statement 1 is correct while Statement 2 is incorrect.
d. Statement 1 is correct while Statement 2 is incorrect.
True or False.
To control the impact of environmental facts, you need to ensure that the bacteria will survive in optimum conditions.
True
True or False.
You should maintain reproducibility and consistency of results so that the same organism will produce the same resistance profile.
True
True or False.
You should maintain reproducibility and consistency of results so that the same organism will produce the same resistance profile.
True
All are standardized and controlled components of AST except:
a. Antimicrobial concentration testing
b. Growth Medium
c. Incubation temperature
d. Bacterial specification
e. Incubation atmosphere
f. Incubation duration
g. Bacterial inoculum size
d. Bacterial specification
Standard AST inoculum size
1.5 x 10 ^8 CFU/mL
Known standard for turbidity comparison
0.5% McFarland Standard
Too much organism might yield _____________ result because bacteria is overpowering the antibiotic.
false-resistant
Too little organism might yield ____________ result to antibiotic.
false-susceptiblity
Most frequently used base medium.
Meuller-Hinto Agar (MHA)
True or False.
Too much calcium and magnesium in the plate, it will compete with the reactivity between bacteria and antibiotic.
True
It cannot settle with plain MHA base and needs a little supplements like 5% sheep or horse blood.
Fastidious bacteria
C. bacter likes what level of incubation temperature?
42 degrees celsius
Incubation duration for AST
16-20 hours
How many times should you perform antimicrobial disc quality testing?
Every week
All are conventional susceptibility testing methods except:
a. disk diffusion
b. d-test
c. broth dilution
d. agar dilution
b. d-test
Two important requirements for appropriate inoculum preparation include:
(1) use of a pure culture [identified bacteria]
(2) a standardized inoculum. [0.5% McFarland Standard]
Suspend/inoculate in broth or ____________ until suspension is turbid
0.85% sterile saline
Turbidity standard inoculum
0.5 MacFarland Standard
True or False.
Use prepared suspension within 15 minutes of preparation.
True
True or False.
Use prepared suspension within 60 minutes of preparation
True
Another term for Disk Diffusion Method
Kirby-Bauer Method
Method that test on organism against multiple antimicrobials
Disk Diffusion Method
