Antimicrobial Susceptibility Testing

Question 1

A procedure used to produce antimicrobial susceptibility profile.

Answer 1

Antimicrobial Susceptibility Testing (AST)

Question 2

A procedure that detects resistance to agents that may be used therapeutically.

Answer 2

Antimicrobial Susceptibility Testing (AST)

Question 3

Performed after every culture and identification of the specimen.

Answer 3

Antimicrobial Susceptibility Testing (AST)

Question 4

All are reasons why AST is important, except:
a. Provides data on the susceptibility of bacterial isolates
b. Assists the clinician in selecting a specific antibiotic therapy
c. Does not avoid antibiotic resistance especially if infection is reoccuring.
d. Replaces very expensive, empirically chosen broad spectrum antibiotics.

Answer 4

c. Does not avoid antibiotic resistance especially if infection is reoccuring.

Question 5

All are standards for AST except:
a. Antibiotic Resistance
b. Thickness of media
c. Nutrients of media
d. Antibiotic

Answer 5

a. Antibiotic Resistance

Question 6

Action should only be between the antibiotic disc and the bacteria of interest. No environment consideration is required in growing bacteria.

a. Both statements are correct.
b. Statement 1 is incorrect while Statement 2 is correct.
c. Both statements are incorrect.
d. Statement 1 is correct while Statement 2 is incorrect.

Answer 6

d. Statement 1 is correct while Statement 2 is incorrect.

Question 7

True or False.

To control the impact of environmental facts, you need to ensure that the bacteria will survive in optimum conditions.

Answer 7

True

Question 8

True or False.

You should maintain reproducibility and consistency of results so that the same organism will produce the same resistance profile.

Answer 8

True

Question 8

True or False.

You should maintain reproducibility and consistency of results so that the same organism will produce the same resistance profile.

Answer 8

True

Question 9

All are standardized and controlled components of AST except:
a. Antimicrobial concentration testing
b. Growth Medium
c. Incubation temperature
d. Bacterial specification
e. Incubation atmosphere
f. Incubation duration
g. Bacterial inoculum size

Answer 9

d. Bacterial specification

Question 10

Standard AST inoculum size

Answer 10

1.5 x 10 ^8 CFU/mL

Question 11

Known standard for turbidity comparison

Answer 11

0.5% McFarland Standard

Question 12

Too much organism might yield _____________ result because bacteria is overpowering the antibiotic.

Answer 12

false-resistant

Question 13

Too little organism might yield ____________ result to antibiotic.

Answer 13

false-susceptiblity

Question 14

Most frequently used base medium.

Answer 14

Meuller-Hinto Agar (MHA)

Question 15

True or False.

Too much calcium and magnesium in the plate, it will compete with the reactivity between bacteria and antibiotic.

Answer 15

True

Question 16

It cannot settle with plain MHA base and needs a little supplements like 5% sheep or horse blood.

Answer 16

Fastidious bacteria

Question 17

C. bacter likes what level of incubation temperature?

Answer 17

42 degrees celsius

Question 18

Incubation duration for AST

Answer 18

16-20 hours

Question 19

How many times should you perform antimicrobial disc quality testing?

Answer 19

Every week

Question 20

All are conventional susceptibility testing methods except:
a. disk diffusion
b. d-test
c. broth dilution
d. agar dilution

Answer 20

b. d-test

Question 21

Two important requirements for appropriate inoculum preparation include:

Answer 21

(1) use of a pure culture [identified bacteria]
(2) a standardized inoculum. [0.5% McFarland Standard]

Question 22

Suspend/inoculate in broth or ____________ until suspension is turbid

Answer 22

0.85% sterile saline

Question 23

Turbidity standard inoculum

Answer 23

0.5 MacFarland Standard

Question 24

True or False.

Use prepared suspension within 15 minutes of preparation.

Answer 24

True

Question 25

True or False.

Use prepared suspension within 60 minutes of preparation

Answer 25

True

Question 26

Another term for Disk Diffusion Method

Answer 26

Kirby-Bauer Method

Question 27

Method that test on organism against multiple antimicrobials

Answer 27

Disk Diffusion Method