Antihumanglobulin Test

Question 1

Reactivity of AHG to cells sensitized with warm antibodies resulted:

Answer 1

Anti-gamma globulin fraction

Question 2

Direct antiglobulin test is also called

Answer 2

One-stage procedure

Question 3

Early AHG reagent were prepared using

Answer 3

Crude globulin fraction

Question 4

Reactivity of AHG to cells sensitized with cold antibodies resulted:

Answer 4

Anti-nongamma globulin fraction

Question 7

The nongamma globulin component was shown to be:

Answer 7

Beta globulin fraction

Question 8

Antiglobulin reagent can either be:

Answer 8

Monospecific or Polyspecific

Question 9

Polyspecific

Answer 9

It contain an antibody to human IgG and to the C3d component of human complement

Question 10

Also called as a two-stage technique

Answer 10

Indirect antiglobulin test

Question 11

Reagent labeled with anti-IgG contain

Answer 11

no anticomplement activity

Question 13

Monospecific reagent contain

Answer 13

only one antibody specificity: either anti-IgG or antibody to specific complement component

Question 14

Anti-IgG contain antibodies specifc to

Answer 14

Fc fragment of the gamma heavy chain

Question 15

If reagent not labeled with “gamma heavy chain-specific” it may contain:

Answer 15

Anti-light chain specificity

Question 16

Anti-light chain specificity react with cells sensitized with

Answer 16

IgM, IgA and IgG

Question 17

The final degradation step has shown to occur:

Answer 17

in-vivo

Question 18

Hemagglutination; indicates..

Answer 18

that the RBC’s had been sensitized by an antibody that had reacted with an antigen present os cell surface

Question 19

What is Block Titrations

Answer 19

performed by reacting dilutions of each antibody to cells sensitized with different amounts of IgG

Question 20

Anti-C3c is considered the most imp anticomplement beacause…

Answer 20

Because of its limited capacity to cause nonspecific reactions

Question 22

ENGELFREIT: Showed that degradtion of C3b to C3d can occur in-vitro if:

Answer 22

incubation is greater than 1 hours

Question 23

Anticomplement use in international reference

Answer 23

Anti-C3d

Question 24

Sensitization can occur:

Answer 24

In-vitro or In-vivo

Question 25

They contain no activity agains human immunoglobulins

Answer 25

Anticomplement

Question 26

Complement activation:

Answer 26

C3 and C4 are divided into two component.. C3b and C4b binds to the RBC membrane. C3a and C4a pass a fluid phase. And further degradation of C3b and C4b occurs by removing C3c and C4c to leave C3d and C4d

Question 27

The cross linking of sensitized RBC’s by AHG produced

Answer 27

Hemagglutination

Question 29

Considered the most important anticomplement component by the ISBT:

Answer 29

anti-C3c

Question 31

Antiglobulin test can be used to detect RBC’s sensitized:

Answer 31

IgG alloantibodies
IgG autoantibodies
Complement component