Antiglobin Testing Flashcards
1
Q
Antiglobulin Test
A
Method to detect IgG antibodies
2
Q
Primary Sensitization
A
- LISS, PEG, Enz for antibody uptake at 37°C
3
Q
Secondary Sensitization
A
uses anti-IgG antibody as a secondary antibody
4
Q
What occurs during incubation? 37°
A
Primary sensitization
Ab in plasma is binding to Ag present on RBC
5
Q
Wash step
A
Washes away any unbound IgG antibodies (there should be lots present)
6
Q
Indirect Antiglobulin Test (IAT) principle
A
7
Q
Coombs control cells
- when it is used
- what it proves
- expected result
A
8
Q
Antihuman Globulin (AHG)
A
9
Q
Polyspecific vs Monospecific
A
10
Q
False positives involved with Antiglobulin testing
A
11
Q
False negatives involved with Antiglobulin testing
A
