Antibodies

Question 1

sedimentation coefficient

Answer 1

7s to 19s

Question 2

criteria of infections

Answer 2

should interfere with normal functioning of body,

somehow cause damage

Question 3

how many disulphide bonds are seen in antibodies

Answer 3

15-20

Question 4

how do you break the disulphide bonds in an antibody

Answer 4

beta mercaptoethanol,

dithiothretol

reduced glutathione

iodoacetate

Question 5

pallad experiment

Answer 5

reduce the disulphide bond and then SDS page

2 band formed.
heavy chain and the light chain

Question 6

if antibody is treated with following enzymes

1. papien
2. pepsin

Answer 6

1, Fab and Fc part seperate

2. F(ab)2’

Question 7

anti heavy chain can react with

Answer 7

Fab and Fc both

Question 8

anti light chain antibody can react with

Answer 8

Fab only

Question 9

Fab is made up of

Answer 9

light chain and heavy chain both

Question 10

Fc is made up of

Answer 10

heavy chain only

Question 11

Fab speciality

Answer 11

have variable region

Question 12

Fc speciality

Answer 12

1 activator for compliment

2. bind with Fc region of other phagocytotic cells.

Question 13

variable region specialities

Answer 13

single domain of 110 aa

having hyper variable regions (3 in no.) also known as CDRs

Question 14

constant region composition of M,E and D,A,G

Answer 14

ME
CH1 to 4

DAG
CH1, hinge, CH2 CH3

Question 15

Hinge region is rich in and its main function is

Answer 15

rich in cystine and proline

and the function is of Flexibility

Question 16

size of IgM, IgE

size of IgD,A,G

Answer 16

1540 aa

1320 aa

Question 17

what is not present in Fc?

Answer 17

CH1 (it is present in Fab)

Question 18

V region is glycosylated. why?

Answer 18

glycosylation helps in the epitope binding.

Question 19

how many CDRs does a IgG have?

Answer 19

12 ( 1 Variable region has 3 CDRs)

Question 20

which drug inhibits N linked glycosylation

Answer 20

tunicamycin

Question 21

Some the features of IgM

Answer 21

largest antibody (pentamer)

highest antibody valency

1st to develop in foetus and on the b cell

MOST POTENT COMPLIMENT ACTIVATOR

Question 22

IgD are produced by

an write down its features

Answer 22

Nasal epithelia and plasma b cells

half life is 2-8 days
second ab produced on b cells
NOT COMPLIMMENT ACTIVATORS

Question 23

IgG types

arrange them on transplacental transfer and compliment activation properties

Answer 23

1 to 4

transplacental tranfer 1,3,4>2

compliment activator 3>1>2>4

Question 24

IgE characteristics

Answer 24

involved in allergy

hypersenstivity type 1 reaction

Question 25

IgA characteristics

Answer 25

least specificity

most secretions per day
(tears, saliva, mother’s milk)

have two type out which one can support the compliments

Question 26

which antibody is a dimer and under what conditions/

Answer 26

IgA
only when it is about to be secreted.

they are joined by the j chains when they come out they secretory parts

Question 27

SDS PAGE RESULTS OF

1. IgG
2. IgM
3. IgA

Answer 27

1. 2 bands
2. 3 bands
3. 4 bands

Question 28

who discovered the structure of antibodies

Answer 28

Edelman

Question 29

though Fab is made up of both heavy and light chain, which variable chain is mostly involved in the antigen binding

Answer 29

heavy variable chain

Question 30

which compliment protein binds to the IgG

Answer 30

C3a proteins.