Antibodies Flashcards
Antibodies roles
- Prevents the pathogen from entering or damaging cell by binding to the.
- Stimulates removal of the pathogens by macrophages or other cells by recognising and coating the pathogen
- Trigger destruction of the pathogen by stimuli ting other immune responses eg complement immune response
Region of the antigen recognised by antibody
Epitope
Devlin of the antibody that ineracts wuth epitope
Paratope
What is contained with paratope
Antigen binding site
CDRS
What are antigen chains usually held together by
Disulphide bonds - inter and intra chain
Draw rough structure and label of antigen
H
In a standard antibody how many cdrs are present
3CDRS in each heavy chain and light chain = 12 in total
What is framework region for
Help maintain folding and structure of cdr
Helps binding of CDR to antigen
Immunoglobulin classes
X
Immunoglobulin sub classes within classes
IgG- 1234
IgA- 1+2
Types of human light chain
Kappa and lambda (2/3 and 1/3) diff subclasses of both can also exists
Diff types of antibody
Polyclonal
Monoclonal (majority)
Recombinant
What is hybridoma technology
Plasma b cell and cancer cells together (malignant plasma cell)- hybrids plasma cell that produces ab forever
What is contained in HAT media
Hypoxanthine
Aminopterin
Thyamide
How is polyclonal antibodies produced
D
