Antibiotics

Question 1

Puromycin

Answer 1

Causes premature termination in both eukaryotes and prokaryotes

Question 2

Cycloheximide

Answer 2

Inhibits peptidyl transferase (60s) in eukaryotes

Question 3

Chloramphenicol

Answer 3

Inhibits peptidyl transferase (50s) in prokaryotes and mitochondria. SEVERE INFECTION ONLY.

Question 4

Streptomycin

Answer 4

Interacts with S-12 (30s); inhibits initiation and causes misreading of mRNA in prokaryotes. In streptomycin resistant strains of bacteria, S12 is altered so streptomycin cant bind the bacterial ribosome.

Question 5

Tetracyclines

Answer 5

Interact with 30S ribosomal subunit and inhibit binding of incoming AA-tRNA to “A” site in prokaryotes

Question 6

Erythromycin

Answer 6

Interacts with 50S ribosomal subunit to block exit tunnel and release of nascent polypeptide in prokaryotes. In resistant strains, 23S rRNA is methylated and erythro no longer binds to 50S.