Animal Models Flashcards
Give the papers and the animals and voltage techniques used in SAN electrical activity
Yanighara & Irisara 1980 - studied the properties of HCN channel - voltage clamp
DiFrancesco 1986 - Rabbit SAN cells - single cell voltage clamp
Bucchi 2007 Rabbit SAN cells - single cell voltage clamp
Milanesi 2006 - HCN4 mutation in FBS (s672A) in HEK273 cells
D’Souza 2014 - trained and sedentary mice
Lakatta 2010
Lakatta & Maltsev 2012
Maltsev 2013 (Gao 2013)
Give the papers and the animals and voltage techniques used in ventricular electrical activity
Brown 1981 - rat myocytes, double suction voltage clamp technique
Carmeliet & Vereecke 1969 - Cow purkinje fibres with TTX treatment
Antzelevitch 2011 - canine left ventricular multicellular wedge
Liu, Gintant & Antzelevitch 1993 - canine left ventricular free wall - whole cell patch clamp
Mitchell 1983 - rat ventricular myocytes - single cell
Mitchell 1984 - rat ventricular myocytes - nifedipine
Janvier & Boyett 1996
LeGuennac & Noble 1994 guinea pig cells, rapid solution exchanger
Sanguinetti & Jurkiewicz 1990 - guinea pig cells - suction voltage clamp technique
Lopartin 1994 - inserted Kir2.3 into HEK cells to show rectification properties
Vandenberg 1987
Give the papers and the animals and voltage techniques used in calcium control and CICR
Ringer 1883
Hirano 1989 - canine atrial cells, rabbit ventricular cells to study Ical and Icat
Reyes-Peres 1989 - transfected cDNA of alpha subunit into mouse L-cells to see current
Sipido 1997 - NCX sufficient for AP - guinea pig cells - disagreed by Levi 1994
Ebashi 1961 - identified relaxing factor - mechanism and due to phosphorylation of Asp371
Clarke 1989 - oligonucleotide mutagenesis of amino acids showed that binding of Ca at M4-M6 and M8 with ATP and change in affinity
Sham 1991 - monoclonal antibody for PLB - in guinea pig cells - whole cells patch clamp - suppresses increase in isopreterenol in calcium uptake like B1 AR
Serysheva 1999 - 3D cryoelectron microscopy showed the structure of the RyR receptor and that it takes an ATP in the presence of Ca for activation
Give the papers and the animals and voltage techniques used in Autoregulation and local control theory
Overend 1998 - assessed affect of tetracaine on muscle contraction - rat perforated Amphotericin A
Wier 1994 - rat ventricle showed that SR Ca release depedent on Ical
Rios-Pizzaro 1989 - suggested it is due to Em in Amphibian myocytes
Cheng 1996 - confocal microscopy of rats - showed Ca sparks capable of CICR
Collier 1999 - confocal microscopy of rats - nifedipine given to reduce Ca transient to visualised individual sparks
Lukyanenko 2001 - saponin permeabilised rat hearts - treated with drugs - Ca viewed by confocal microscopy
Give the papers and the animals and voltage techniques used in CPVT
Yang 2006 - introduced DCP10 into rat myocytes - permeabilised patch clamp
Thomas 2004 - WT and RyR2 mutant into HEK cells - reduced threshold to AP and sensitivity
Watanabe 2009 - Mice model Casq2 WT and KO - flecainide treatment
Hilliard 2010 - permeabilised patch clamp mice model of Casq2, spark mass and frequency, sheep heart bilayers
Sikkel 2013 - rat perforated patch clamp - mechanism due to Na antiarrhythmic effect only
Bannister 2015 - bilayer with WT RYR2 receptor showed no inhibition
Give the papers and the animals and voltage techniques used in stretch change in cardiac output
Alvarez 1999 - rat trabeculae myocytes
McDonald & Moss 1995 - rat ventricular myocytes
Konhilas 2002 - rat trabeculae myocytes
Fukada 2001 - rat trabeculae
Cazorla 1999 - rat ventricular
Farman 2010 - WT and mutant TnC in rat trabeculae
Calaghan & White 2004 - rat ventricular myocytes - HOE and streptomycin given
Caldiz 2007 - cat papillary muscles - ROS
Dyachenko 2009 - murine myocytes - TRPC6
Vila-Petroff 2001 - SR ca release - rat myocytes
Give the papers and the animals and voltage techniques used in stretch change in electrical activity
Franz 2000 - canine myocytes balloon stretch
Cooper 2000 - rabbit SAN
Cooper 2006 - rabbit SAN
Kim 1992 - rat SAN - negative pressure
Hagiwara 1992 - rat SAN - positive pressure
Matsuda 1996 - rabbit SAN - positive pressure
Give the papers and the animals and voltage techniques used in autonomic nervous system
DiFrancesco 1986 - rabbit SAN cells - single cell patch clamp
Lakatta 2008
Yuan & Bers 1995 - Forskolin and H89 treatment on Ical - Serine 1928
Manobe 2014 - BHK cells - Serine 1574
Wegener 1989 - Guinea Pig cells - Serine 16 and Threonine 17
Marks 2000 - Serine 2808 phosphorylated
MacDonnell 2008 - S2808A mouse model - give isopreterenol - inotropic effects
Li 2000 - WT and PLB KO in mice - bradycardic effects - lusitropy
DiFrancesco 1986 - rabbit SAN cells single cell voltage clamp - ACh
Wickman 1998 - mouse K(Ach) KO - GIRK channel
Wang & Lipsius 1995 - Cat atrial cells - whole cell patch clamp - ACh given and then washout for 30s
